The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels

Kim, Suhee; Kim, Donghak; Cha, Misun; Kim, Soo Hyun; Jung, Youngmee

doi:10.3390/ijms222212560

Cited by 13 publications

(12 citation statements)

References 39 publications

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“…Multiple polymers are available for the generation of hydrogels to simulate a cell microenvironment in 3D in vitro adipose tissue, e.g., poly-lactide-caprolactone, polysaccharides, cellulose-based materials, collagens, or hyaluronic acid [ 17 , 18 , 19 , 20 ]. One frequently used material is gelatin methacryloyl (GelMA).…”

Section: Introductionmentioning

confidence: 99%

Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells

Albrecht

Schmidt

Volz

et al. 2022

Gels

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Adipose tissue is related to the development and manifestation of multiple diseases, demonstrating the importance of suitable in vitro models for research purposes. In this study, adipose tissue lobuli were explanted, cultured, and used as an adipose tissue control to evaluate in vitro generated adipose tissue models. During culture, lobule exhibited a stable weight, lactate dehydrogenase, and glycerol release over 15 days. For building up in vitro adipose tissue models, we adapted the biomaterial gelatin methacryloyl (GelMA) composition and handling to homogeneously mix and bioprint human primary mature adipocytes (MA) and adipose-derived stem cells (ASCs), respectively. Accelerated cooling of the bioink turned out to be essential for the homogeneous distribution of lipid-filled MAs in the hydrogel. Last, we compared manual and bioprinted GelMA hydrogels with MA or ASCs and the explanted lobules to evaluate the impact of the printing process and rate the models concerning the physiological reference. The viability analyses demonstrated no significant difference between the groups due to additive manufacturing. The staining of intracellular lipids and perilipin A suggest that GelMA is well suited for ASCs and MA. Therefore, we successfully constructed physiological in vitro models by bioprinting MA-containing GelMA bioinks.

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Section: Introductionmentioning

confidence: 99%

Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells

Albrecht

Schmidt

Volz

et al. 2022

Gels

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show abstract

“…ADSCs served as usual tools for vascularization in tissue engineering because of their convenient management, ability to self-renew and differentiate into various cell types, and secretion of angiogenic factors [ 31 , 32 ]. ADSCs-seeded silk fibroin scaffolds or hydrogels that accelerated angiogenesis were reported [ 33 , 34 ]. Our previous study also reported that ADSCs-seeded-PADM promoted pro-vascular gene expression and increased vascular infiltration [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

Frozen bean curd-inspired xenogeneic acellular dermal matrix with triple pretreatment approach of freeze–thaw, laser drilling and ADSCs pre-culture for promoting early vascularization and integration

Huang

Zhu

Lin

et al. 2022

Regenerative Biomaterials

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Xenogeneic acellular dermal matrix (ADM) is widely used in clinical practice given its good biocompatibility and biomechanical properties. Yet, its dense structure remains a hindrance. Incorporation of laser drilling and pre-culture with Adipose-derived stem cells (ADSCs) have been attempted to promote early vascularization and integration, but the results were not ideal. Inspired by the manufacturing procedure of frozen bean curd, we proposed a freeze-thaw treatment to enhance the porosity of ADM. We found that the ADM treated with -80°C3R+-30°C3R had the largest disorder of stratified plane arrangement (deviation angle 28.6%) and the largest porosity (96%), making it an optimal approach. Human umbilical vein endothelial cells on freeze-thaw treated ADM demonstrated increased expression in Tie-2 and CD105 genes, proliferation, and tube formation in vitro compared with those on ADM. Combining freeze-thaw with laser drilling and pre-culture with ADSCs, such tri-treatment improved the gene expression of pro-angiogenic factors including IGF-1, EGF, and VEGF, promoted tube formation, increased cell infiltration, and accelerated vascularization soon after implantation. Overall, freeze-thaw is an effective method for optimizing the internal structure of ADM, and tri-treatments may yield clinical significance by promoting early cell infiltration, vascularization, and integration with surrounding tissues.

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“…Kim et al . used poly (l-lactide-co-caprolactone) scaffolds combined with DAT and ASCs to successfully prepare scaffolds that can promote adipogenesis and angiogenesis [ 29 ]. By combining bioresorbable polycaprolactone and DAT, Zhang et al .…”

Section: Discussionmentioning

confidence: 99%

Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration

et al. 2023

Burns &Amp; Trauma

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Background Large-area soft tissue defects are challenging to reconstruct. Clinical treatment methods are hampered by problems associated with injury to the donor site and the requirement for multiple surgical procedures. Although the advent of decellularized adipose tissue (DAT) offers a new solution to these problems, optimal tissue regeneration efficiency cannot be achieved because the stiffness of DAT cannot be altered in vivo by adjusting its concentration. This study aimed to improve the efficiency of adipose regeneration by physically altering the stiffness of DAT to better repair large-volume soft tissue defects. Methods In this study, we formed three different cell-free hydrogel systems by physically cross-linking DAT with different concentrations of methyl cellulose (MC; 0.05, 0.075 and 0.10 g/ml). The stiffness of the cell-free hydrogel system could be regulated by altering the concentration of MC, and all three cell-free hydrogel systems were injectable and moldable. Subsequently, the cell-free hydrogel systems were grafted on the backs of nude mice. Histological, immunofluorescence and gene expression analyses of adipogenesis of the grafts were performed on days 3, 7, 10, 14, 21 and 30. Results The migration of adipose-derived stem cells (ASCs) and vascularization were higher in the 0.10 g/ml group than in the 0.05 and 0.075 g/ml groups on days 7, 14 and 30. Notably, on days 7, 14 and 30, the adipogenesis of ASCs and adipose regeneration were significantly higher in the 0.075 g/ml group than in the 0.05 g/ml group (p < 0.01 or p < 0.001) and 0.10 g/ml group (p < 0.05 or p < 0.001). Conclusion Adjusting the stiffness of DAT via physical cross-linking with MC can effectively promote adipose regeneration, which is of great significance to the development of methods for the effective repair and reconstruction of large-volume soft tissue defects.

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The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels

Cited by 13 publications

References 39 publications

Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells

Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells

Frozen bean curd-inspired xenogeneic acellular dermal matrix with triple pretreatment approach of freeze–thaw, laser drilling and ADSCs pre-culture for promoting early vascularization and integration

Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration

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