The regeneration and augmentation of bone with injectable osteogenic cell sheet in a rat critical fracture healing model

Shimizu, Takamasa; Akahane, Manabu; Morita, Yusuke; Omokawa, Shohei; Nakano, Kazuhiko; Kira, Tsutomu; Onishi, Tadanobu; Inagaki, Yusuke; Okuda, Akinori; Kawate, Kenji; Tanaka, Yasuhito

doi:10.1016/j.injury.2015.04.031

Cited by 43 publications

(30 citation statements)

References 35 publications

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“…In previous reports, osteogenic cell sheets with pin fixation were found to promote bone healing by large callus formation at the fracture site [15,19]. In the present study, abundant external callus was found in the control group compared to UCS and OCS groups, although the fixation was more rigid with bone plates and screws unlike pins used in previous studies.…”

Section: Discussionsupporting

confidence: 45%

“…Pins have been used as an internal fixation material in studies for evaluation of effect of cell sheets on bone regeneration because more rigid fixation such as plates and screws could not be applied in experimental animals such as mice, rats and rabbits [15,[17][18][19][22][23][24]. The bone healing pathway by unstable fixation like pins is expected to be secondary bone healing due to cartilaginous callus formation however there is no primary bone healing through intramembranous ossification [25].…”

Section: Introductionmentioning

confidence: 99%

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Different Bone Healing Effects of Undifferentiated and Osteogenic Differentiated Mesenchymal Stromal Cell Sheets in Canine Radial Fracture Model

Yoon

Khan

Choi

et al. 2017

Tissue Eng Regen Med

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Cell sheets technology is being available for fracture healing. This study was performed to clarify bone healing mechanism of undifferentiated (UCS) and osteogenic (OCS) differentiated mesenchymal stromal cell (MSC) sheets in the fracture model of dogs. UCS and OCS were harvested at 10 days of culture. Transverse fractures at the radius of six beagle dogs were assigned into three groups (n = 4 in each group) i.e. UCS, OCS and control. The fractures were fixed with a 2.7 mm locking plate and six screws. Cell sheets were wrapped around the fracture site. Bones were harvested 8 weeks after operation, then scanned by micro-computed tomography (micro-CT) and analyzed histopathologically. The micro-CT revealed different aspects of bone regeneration among the groups. The percentages of external callus volume out of total bone volume in control, UCS, and OCS groups were 42.1, 13.0 and 4.9% (p \ 0.05) respectively. However, the percentages of limbs having connectivity of gaps were 25, 12.5 and 75% respectively. In histopathological assessments, OCS group showed well organized and mature woven bone with peripheral cartilage at the fracture site, whereas control group showed cartilage formation without bone maturation or ossification at the fracture site. Meanwhile, fracture site was only filled with fibrous connective tissue without endochondral ossification and bone formation in UCS group. It was suggested that the MSC sheets reduced the quantity of external callus, and OCS induced the primary bone healing.

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Section: Discussionsupporting

confidence: 45%

Section: Introductionmentioning

confidence: 99%

Different Bone Healing Effects of Undifferentiated and Osteogenic Differentiated Mesenchymal Stromal Cell Sheets in Canine Radial Fracture Model

Yoon

Khan

Choi

et al. 2017

Tissue Eng Regen Med

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“…Femoral defect model has been regarded as an appropriate experimental model of bone regeneration. In femoral defect model, surgical procedures can be relatively simple to accomplish, preperation of tissue speciemen is easy, observations can be focused on healing process of bone [14][15][16][17][18] . For enhancing bone regeneration and healing performance, several treatment modalities and more recently ozone were applied.…”

Section: Discussionmentioning

confidence: 99%

Effects of Gaseous Ozone Treatment on Bone Regeneration in Femoral Defect Model in Rats

Duman

Davul

Gökçe

et al. 2017

J. Hard Tissue Biology.

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The purpose of this study was to investigate the effect of ozone therapy in rat femoral fractures.Following the right open femoral fractures and intramedullary Kirschner wire fixation of the fracture in 60 male Wistar albino rats, the animals were divided into two groups of 30 animals each: ozone therapy group (n=30); non-treatment (control) group (n=30). Ozone was administered by rectal insufflation. After the rats were sacrified fracture healing was assessed by biomechanical and histopathologic evaluation. Histological and mechanical findings indicated that the ozone therapy had a significantly positive effect on bone healing.

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“…A promising new technology in which cell sheets are produced has been used for regenerating various organs . Instead of obtaining single or aggregated cells in suspension, cell sheet products are comprised of a layer of monolayer cultured cells .…”

Section: Introductionmentioning

confidence: 99%

Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model

et al. 2019

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In recent years, studies have explored novel approaches for cell transplantation to enable annulus fibrosus (AF) regeneration of the intervertebral disc in particular for lumbar disc herniation. Nevertheless, successful engraftment of cells is structurally challenging, and no definitive method has yet been established. This study investigated the potential of cell sheet technology to facilitate cell engraftment for AF repair. AF injury was induced by a 1 × 1 mm defect in rat tails after which AF cell sheets were transplanted. Its regenerative effects were compared to a nondegenerated and degeneration only conditions. Degenerative changes of the entire intervertebral disc were examined by disc height measurements, histology, and immunohistochemistry for 4‐, 8‐, and 12‐weeks post‐transplantation. Cell engraftment was confirmed by tracing PKH26 fluorescent dyed AF cells. In the transplant group, disc degeneration was significantly suppressed after 4, 8, and 12 weeks when compared with the degenerative group, as indicated by histological scoring and DHI observations. At 2 and 4 weeks after transplant, PKH26 positive cells could be detected in defect region and surrounding AF. The results suggest cell engraftment into AF tissue could be established by the cell sheet technology without additional scaffolding or adhesives. In short, AF cell sheets appear to be an effective and accessible tool for AF repair and to support intervertebral disc regeneration.

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The regeneration and augmentation of bone with injectable osteogenic cell sheet in a rat critical fracture healing model

Cited by 43 publications

References 35 publications

Different Bone Healing Effects of Undifferentiated and Osteogenic Differentiated Mesenchymal Stromal Cell Sheets in Canine Radial Fracture Model

Different Bone Healing Effects of Undifferentiated and Osteogenic Differentiated Mesenchymal Stromal Cell Sheets in Canine Radial Fracture Model

Effects of Gaseous Ozone Treatment on Bone Regeneration in Femoral Defect Model in Rats

Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model

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