The REFOLD database: a tool for the optimization of protein expression and refolding

Chow, Michelle K M; Amin, Abdullah Al; Fulton, Kate Frances; Fernando, Thushan; Kamau, Lawrence; Batty, C.J.; Louca, Michael; Ho, Storm; Whisstock, James C.; Bottomley, Stephen P.; Buckle, Ashley M.

doi:10.1093/nar/gkj080

Cited by 44 publications

(23 citation statements)

References 15 publications

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“…Notwithstanding, efforts have been made to rationalize the refolding step, the free REFOLD database (http://refold.med.monash.edu.au; Chow et al, 2006a,b) can be a useful tool for the design of procedures for the refolding and purification of recombinant proteins.…”

Section: Methods For the Recovery Of Functional Proteins From Ibsmentioning

confidence: 99%

Inclusion bodies: not that bad…

2014

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The formation of inclusion bodies (IBs) constitute a frequent event during the production of heterologous proteins in bacterial hosts. Although the mechanisms leading to their formation are not completely understood, empirical data have been exploited trying to predict the aggregation propensity of specific proteins while a great number of strategies have been developed to avoid the generation of IBs. However, in many cases, the formation of such aggregates can be considered an advantage for basic research as for protein production. In this review, we focus on this positive side of IBs formation in bacteria. We present a compilation on recent advances on the understanding of IBs formation and their utilization as a model to understand protein aggregation and to explore strategies to control this process. We include recent information about their composition and structure, their use as an attractive approach to produce low cost proteins and other promising applications in Biomedicine.

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Section: Methods For the Recovery Of Functional Proteins From Ibsmentioning

confidence: 99%

Inclusion bodies: not that bad…

2014

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“…The critical problems are misfolding and aggregation, respectively. How to cope with these limitations in detail is out of the scope of this chapter and is discussed in specialized texts (60)(61)(62)(63)(64).…”

Section: The Use Of Microspheres In Protein Refoldingmentioning

confidence: 99%

Microcapsules for Cell Transplantation: Design, Preparation, and Application

Su¹

2013

Microspheres and Microcapsules in Biotechnology

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“…Chow and colleagues have devised a set of rules that can be applied to many proteins, and this is available as a web server [31]. Using a refolding method, we have compared the in vitro enzymatic activity of cathepsin L1g expressed in either yeast of E. coli.…”

Section: Choosing a Systemmentioning

confidence: 99%

So you Need a Protein - A Guide to the Production of Recombinant Proteins

Jayaraj¹,

Smooker

2009

TOVSJ

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Abstract:The field of biotechnology owes a great deal to the ability to produce recombinant proteins, which can be made in far greater abundance than many native proteins, and are more easily quality controlled. There is a great need for individual proteins to be produced for research purposes. This review is aimed at researchers who are not experienced at protein expression, but find that they have a need to produce a recombinant protein. We detail the major expression systems that will be commonly used in the laboratory situation-bacterial, yeast and insect cell culture. The application of each, and the relative advantages/disadvantages are discussed.

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The REFOLD database: a tool for the optimization of protein expression and refolding

Cited by 44 publications

References 15 publications

Inclusion bodies: not that bad…

Inclusion bodies: not that bad…

Microcapsules for Cell Transplantation: Design, Preparation, and Application

So you Need a Protein - A Guide to the Production of Recombinant Proteins

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