The redox‐sensitive module of cyclophilin 20‐3, 2‐cysteine peroxiredoxin and cysteine synthase integrates sulfur metabolism and oxylipin signaling in the high light acclimation response

Abstract

The integration of redox- and reactive oxygen species-dependent signaling and metabolic activities is fundamental to plant acclimation to biotic and abiotic stresses. Previous data suggest the existence of a dynamically interacting module in the chloroplast stroma consisting of cyclophilin 20-3 (Cyp20-3), O-acetylserine(thiol)lyase B (OASTL-B), 2-cysteine peroxiredoxins A/B (2-CysPrx) and serine acetyltransferase 2;1 (SERAT2;1). The functionality of this COPS module is influenced by redox stimuli and oxophytodienoic acid (OPDA), which is the precursor for jasmonic acid. The concept of an integrating function of these proteins in stress signaling was challenged by combining transcriptome and biochemical analyses in Arabidopsis mutants devoid of oastlB, serat2;1, cyp20-3 and 2-cysprxA/B, and wild-type (WT). Leaf transcriptomes were analyzed 6 h after transfer to light intensity 10-fold in excess of growth light or under growth light. The survey of KEGG-based gene ontology groups showed common upregulation of translation- and protein homeostasis-associated transcripts under control conditions in all mutants compared with WT. The results revealed that the interference of the module was accompanied with disturbance of carbohydrate, sulfur and nitrogen metabolism, and also citric acid cycle intermediates. Apart from common regulation, specific responses at the transcriptome and metabolite level linked Cyp20-3 to cell wall-bound carbohydrates and oxylipin signaling, and 2-CysPrx to photosynthesis, sugar and amino acid metabolism. Deletion of either OASTL-B or SERAT2;1 frequently induced antagonistic changes in biochemical or molecular features. Enhanced sensitivity of mutant seedlings to OPDA and leaf discs to NaHS-administration confirmed the presumed functional interference of the COPS module in redox and oxylipin signaling.

Comparative Proteomic Analysis of Nodulated and Non-Nodulated Casuarina glauca Sieb. ex Spreng. Grown under Salinity Conditions Using Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS)

“…Since antioxidant defense involves the accumulation of two important thiol-containing compounds, cysteine and glutathione [46,47], we can expect sulfur metabolism to change under salt stress imposition. In this context, it is interesting to note that recent studies suggested the existence of a dynamically interacting module in the chloroplast stroma consisting of a peptidylprolyl isomerase (cyclophilin), two 2-cysteine peroxiredoxins A/B and a cysteine synthase, that integrate sulfur metabolism and oxylipin signaling during the acclimation to high light stress [48]. In salt-stressed KNO 3…”

The significance of glutathione and ascorbate in modulating the retrograde high light response in Arabidopsis thaliana leaves

“…Recently, overexpression of non‐specific LTPs was shown to increase glutathione contents and resistance to mycotoxins (McLaughlin et al ). LTP3 and LTP4 transcript levels also respond strongly in H‐light exposed A. thaliana mutants devoid of the chloroplast thiol peroxidases 2‐cys peroxiredoxins A/B (Müller et al ). Similar to the first set of experiments, transcripts were very strongly upregulated in pad 2 exposed to H‐light ( HSP17.4 , HSP17.6a , HSP20‐like2 and LTP3 ) and slightly for HSP20‐like1 , while the ascorbate deficiency increased LTP3/4 levels.…”

Plant–Pathogen Interactions: Underestimated Roles of Phyto-oxylipins

“…In pathogen-infected plants, RES have been reported to stimulate the expression of defensive genes and to directly modify proteins [71]. For example, OPDA has been reported to bind cyclophilin 20-3 (CYP20-3), a binding protein that regulates stress-responsive cellular redox homeostasis [72,73]. Regardless of the defence route, it is well known that the excessive production of electrophilic molecules can disrupt natural cellular functions and, eventually, cause cell collapse [74].…”