The Red Pulp of the Rabbit Spleen Studied under the Scanning Electron Microscope

Miyoshi, Masayuki; Fujita, Tsuneo; Tokunaga, Junichi

doi:10.1679/aohc1950.32.289

Cited by 20 publications

(14 citation statements)

References 20 publications

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“…Recently WEISS (1974) published an SEM study of the rat spleen confirming largely the findings of our study in this animal (MIYOSHI and FUJITA, 1970). As he perfused the organ not through the arteries but through the interstitium, the surface of the cells in his micrographs were not completely free of blood coagulum.…”

supporting

confidence: 84%

“…In the rabbit (MIYOSHI, FUJITA and TOKUNAGA, 1970), dog and rat (MIYOSHI and FUJITA, 1971) the rod cells of the sinus were demonstrated to be connected by their side processes into a lattice-like plate possessing persistent perforations, thus confirming the descriptions by early light microscopists (MOLLIER, 1911;HARTMANN, 1930). Species differences in the pattern of the lattice were shown (MIYOSHI and FUJITA, 1971) and the occurrence of warty and fibrous microprojections of the rod cell cytoplasm was reported (MIYOSHI, FUJITA and TOKUNAGA, 1970. Billroth cords were demonstrated to be formed by the network of reticulum cells whose meshes contained macrophages and other "free round" cells.…”

supporting

confidence: 75%

“…After our previous papers reported the stereo-fine structures of the red pulp of the rabbit (MIYOSHI, FUJITA and TOKUNAGA, 1970), dog and rat (MIYOSHI and FUJITA, 1971), SUZUKI (1972) published a paper on his SEM observations of the human spleen. He reported that the structures of the sinuses and cords were essentially identical with our findings in animals.…”

Section: Discussionmentioning

confidence: 99%

“…As reviewed elsewhere (MIYOSHI, FUJITA and TOKUNAGA, 1970), it was the light microscopic study by MOLLIER (1911) which exactly described and illustrated the grid pattern of the sinus endothelium formed by the longitudinal cytoplasm of rod cells and their transverse bridges. In our previous studies in some animals we could fully confirm the structure proposed by MOLLIER.…”

Section: Splenic Sinusmentioning

confidence: 96%

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A Scanning Electron Microscope Study of the Human Spleen

Fujita

1974

Arch. Histol. Jap., Arch. Histol. Jpn

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supporting

confidence: 84%

supporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Splenic Sinusmentioning

confidence: 96%

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A Scanning Electron Microscope Study of the Human Spleen

Fujita

1974

Arch. Histol. Jap., Arch. Histol. Jpn

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“…The configuration of the erythrocytes hanging over the collagen fibers has been hitherto reported in the filtering portion of such organs as the spleen (MIYOSHI, FUJITA and TOKUNAGA, 1970;MIYOSHI and FUJITA, 1971). BULL and KUHN (1970) also found erythrocytes quite similarly hanging over fibrins in an experimental study on the production of the schistocytes by fibrins.…”

Section: Effect Of Aspirinmentioning

confidence: 72%

A Scanning Electron Microscope Study on Hemostatic Reaction. Early Hemostatic Plug Formation and the Effect of Aspirin

Hattori

Jinbo

Iizumi

et al. 1975

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary. In order to clarify the mechanism of the hemostatic plug formation from the morphologic standpoint, scanning electron microscope observation was made on the inner surface of the rabbit carotid arteries 1, 5, 15, 30 and 45sec after stabbing with a needle. The arteries were first fixed in situ by an infusion of glutaraldehyde and then, after being excised, by immersion in glutaraldehyde and in osmium tetroxide. The critical point-dried and gold-palladium covered specimens were examined in a field emission type scanning electron microscope.Stabbing made a hole, about 0.25mm in diameter, in the arterial wall, and the ruptured endothelial cell shrank and retracted. Thus the subendothelial collagen fibers were exposed and hung on the wall of the holes. Some of the fibers were single and wavy and others were compactly packed in bundles, forming a network.The first step of the hemostatic process was adhesion of discoid platelets which were attached, with their bodies or frequently with their pseudopods, to the collagen fibers especially at the marginal and hanging part.On the adhered platelets other free platelets subsequently came to be attached. A thin layer of the platelet aggregates thus formed grew thicker, though somewhat distorted by the blood stream. At this second stage some erythrocytes were found hanging from the collagen fibers or undergoing collapse, suggesting its contribution to platelet aggregation by releasing ADP through hemolysis. The aggregate layer became so thick that it almost obliterated the hole and rose on the arterial lumen 45 sec after the stabbing.Aspirin which has been shown to affect platelet aggregation in the second phase was demonstrated to lower the rate of aggregate formation to half to one-third.

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Electron microscopy of the red pulp of human spleen

Chen

Weiss

1972

Am. J. Anat.

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Terminating arterial vessels, the structure of sinuses and cords, and the passage of cells through the sinus wall in the red pulp of human spleen were studied. All terminating arterial capillaries observed opened into cords. The distance be tween terminating arterial capillaries and sinuses vaned. Macrophages were commonly present at arterial terminations. Arterial capillary endothelium contained filaments about 80 A in diameter. Blood cells were frequently present in the capillary lumen or in passage through the capillary wall into cords. Endothelial cells of sinuses contained three distinctive structures : loosely organized cytoplasmic filaments, tightly organized finer filaments, and micropinocytotic vesicles. Many micropinocytotic vesicles about 0.1 , u in diameter were present just. beneath the plasma membrane of the lateral and luminal sides of sinus endothelial cells and a few at the basal aspect. Loosely organized filaments about 80 A in diameter ran parallel to the longitudinal axis of the sinus endothelium. The finer filaments about 30-50 A in diameter were tightly organized as filamentous bands and present basally. The filaments of the bands appeared inserted upon the plasma membrane. They were also present in the cordal reticular cells and terminating arterial capillaries. Free cells were frequently present in passage through the slits of the sinus wall. There were no preformed or fixed apertures in the sinus wall. The basement membrane and reticular fibers were completely covered by the endothelial cells and/or cordal reticular cells.It is likely that those slits between endothelial cells in the sinus wall not covered by the basement membrane are potential passageways for cells moving from the cords into the sinus. The larger cytoplasmic filaments are likely contractile. The filamentous bands appear to maintain cell shape, stabilize the wall in relation to the basement membrane, and are probably operative in the control of cellular passage through the slits of sinus wall.

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The Red Pulp of the Rabbit Spleen Studied under the Scanning Electron Microscope

Cited by 20 publications

References 20 publications

A Scanning Electron Microscope Study of the Human Spleen

A Scanning Electron Microscope Study of the Human Spleen

A Scanning Electron Microscope Study on Hemostatic Reaction. Early Hemostatic Plug Formation and the Effect of Aspirin

Electron microscopy of the red pulp of human spleen

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