The recovery of the testis over 8 weeks after short-term dosing with ethylene glycol monomethyl ether: Histology, cell-specific enzymes, and rete testis fluid protein

Chapin, Robert E.; Dutton, Sandra L.; Ross, Monica D.; Swaisgood, Ronald R.; Lamb, James C.

doi:10.1016/0272-0590(85)90099-5

Cited by 26 publications

(3 citation statements)

References 16 publications

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“…The dose of 2,5-HD was set based on the finding in male rats that administration of more than 700 mg/kg of 2,5-HD as a single dose suppressed sperm motion (Isobe et al, 1998). In the case of EGME, the dose was set based on the absence of histopathological findings in the testes after a single dose of 50 mg/kg and the degeneration of spermatocytes observed after a single dose of 500 mg/ kg (Chapin et al, 1985). In the case of CP, the dose was set based on the absence of morphological lesions for 2-weeks after treatment with 5 mg/kg (Watanabe et al, 2000) and a decrease in spermatogenical cells observed after a single dose of 100 mg/kg (Matsui et al, 1995).…”

Section: Animals and Treatmentmentioning

confidence: 99%

“…EGME is an organic solvent used in paints and printing inks. From the findings of animal studies, it is known that exposure to this compound leads to testicular atrophy and impaired fertility (Chapin et al, 1984(Chapin et al, , 1985, and the target cells seem to be primary spermatocytes in the dividing stage and early and late pachytene stages (Chapin et al, 1984). CP is an anti-cancer drug and treatment with this drug leads to a decrease of spermatogonia in rats (Matsui et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Male Reproductive Toxicants on Gene Expression in Rat Testes

et al. 2005

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Predictive biomarkers of testicular toxicity are needed for an efficient development of drugs. The purpose of the present study was to obtain further insight into the toxicity mechanisms of various male reproductive toxicants and to detect genomic biomarkers for rapid screening of testicular toxicity. Four reproductive toxicants, 2,5-hexanedione (Sertoli cells toxicant), ethylene glycol monomethyl ether (EGME; spermatocytes toxicant), cyclophosphamide (spermatogonia toxicant) and sulfasalazine, were orally administered to male rats once. Six hours after the single dosing, gene expression in the testes was monitored by cDNA microarray and real-time RT-PCR and the testes were histopathologically examined. No histopathological abnormality was detected except for slight degeneration of spermatocytes in the EGME-treated testes. cDNA microarray analysis revealed differential gene expression profiles, and it was possible based on the profiles to characterize the action of the compounds in the testes. Interestingly, 3 spermatogenesis-related genes -- heat shock protein 70-2, insulin growth factor binding protein 3 and glutathione S transferase pi -- were affected by all the compounds. The above changes of gene expression were detectable within a short period after the dosing prior to the appearance of obvious pathological changes. These data suggest that cDNA microarray is a useful technique for evaluation of primary testicular toxicity. Furthermore, we propose the above 3 spermatogenesis-related genes as potential biomarkers of testicular toxicity.

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Section: Animals and Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Male Reproductive Toxicants on Gene Expression in Rat Testes

et al. 2005

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“…LDH‐X is the specific isozyme of LDH in the testes and it is often used as a marker of germ cell maturation (Meistrich, ). Changes in testicular levels of LDH‐X activity have been previously associated with the presence of cytotoxicity induced by many toxicants (Chapin et al, ). The decreased testicular LDH‐X activity, in this study, could be one of the contributory factors leading to reduced sperm concentration and motility.…”

Section: Discussionmentioning

confidence: 99%

Lipoic acid and Calligonum comosumon attenuate aroclor 1260‐induced testicular toxicity in adult rats

Aly

Alahdal

Nagy

et al. 2016

Environmental Toxicology

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Aroclor 1260 is one of the more representative polychlorinated biphenyls found in biota. This study was designed to delineate the testicular toxicity of Aroclor 1260 and to elucidate the potential protective role of Calligonum comosum (C. comosum) and lipoic acid in adult rats. Aroclor 1260 was dissolved in corn oil and given to rats by gavage at doses 0, 20, 40, or 60 mg/kg/day for 15 consecutive days (Groups I, II, III, and IV, respectively). Groups V and VI were pretreated with C. comosum (200 mg/kg/day) and lipoic acid (35 mg/kg/day) respectively 24 h before Aroclor 1260 (40 mg/kg/day) treatment for 15 consecutive days. Aroclor 1260 (20, 40 or 60 mg/kg/day) treatment significantly decreased testes weight, sperm count and motility and daily sperm production. Serum testosterone was significantly decreased in response to treatment with 40 and 60 mg/kg/day of Aroclor 1260. LDH-X activity was significantly decreased at the three dose levels. Hydrogen peroxide (H O ) production (in a dose-related manner) and lipid peroxidation were significantly increased in response to Aroclor 1260 (20, 40, or 60 mg/kg/day) treatment. Aroclor 1260 at the three dose levels decreased the activities of the antioxidant enzymes SOD, CAT, GPx, and GR and the non-enzymatic antioxidant GSH level. CAT, GPx and GSH showed a dose-response effect. These abnormalities were effectively attenuated by pretreatment with C. comosum (200 mg/kg/day) or lipoic acid (35 mg/kg/day). Histopathological examination showed a dose-related increase in morphological abnormalities of the testis in response to Aroclor 1260 treatment. In conclusion, Aroclor 1260 induced testicular toxicity at least, in part, by induction of oxidative stress. By reversal of biochemical and morphological changes towards normalcy, the cytoprotective role of C. comosum and lipoic acid is illuminated. In comparison, lipoic acid was more protective than C. comosum extract against testicular toxicity induced by Aroclor 1260. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1147-1157, 2017.

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Intratesticular localization of the organic solute carrier protein, OSCP1, in spermatogenic cells in mice

Hiratsuka

Yin

Ohtomo

et al. 2008

Molecular Reproduction Devel

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Organic solute carrier protein 1 (OSCP1) is a recently described human gene that facilitates the transport of various organic solutes into the cell, when expressed in frog eggs. In this study, we cloned a mouse ortholog of OSCP1 encoding 379 amino acid protein, with 94% homology to the human counterpart. The mouse OSCP1 mRNA was predominantly expressed in the testis, in which it was attributed to the spermatogenic cells, except the spermatogonia. Immunohistochemistry confirmed that OSCP1 protein is continuously expressed during spermatogenesis in a stage- and cell type-specific manner, in the leptotene spermatocytes at stage IX through step 15 spermatids. Subcellular fractionation of mouse testis homogenates indicated that OSCP1 is a 45-kDa cytosolic protein. Moreover, when green fluorescent protein-OSCP1 fusion constructs were transfected into cultured cells, the fluorescence localized evenly in the cytoplasm. These results suggest that mouse testis OSCP1 may indirectly mediate substrate uptake into meiotic and spermiogenic germ cells, within the cytosol.

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The recovery of the testis over 8 weeks after short-term dosing with ethylene glycol monomethyl ether: Histology, cell-specific enzymes, and rete testis fluid protein

Cited by 26 publications

References 16 publications

Effects of Male Reproductive Toxicants on Gene Expression in Rat Testes

Effects of Male Reproductive Toxicants on Gene Expression in Rat Testes

Lipoic acid and Calligonum comosumon attenuate aroclor 1260‐induced testicular toxicity in adult rats

Intratesticular localization of the organic solute carrier protein, OSCP1, in spermatogenic cells in mice

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