The Recognition of the Nonclassical Major Histocompatibility Complex (MHC) Class I Molecule, T10, by the γδ T Cell, G8

Crowley, Michael P.; Reich, Ziv; Mavaddat, Nasim; Altman, John D.; Chien, Yueh‐hsiu

doi:10.1084/jem.185.7.1223

Cited by 90 publications

(83 citation statements)

References 38 publications

(60 reference statements)

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“…The T m is identical for T18 d folded in the presence or absence of the nonameric peptide library. A second transition with T m Ͼ60°C characterized by a sign reversal of the CD signal probably represents unfolding of free ␤ 2 m (35,40). The 54°C T m of empty T18 d is similar to that reported for H-2K d peptide complexes (54 -57°C) (35,38) and greater than that reported for the class Ib protein T10 (49°C) (40), which does not bind peptides.…”

Section: Thermal Stability Of T18 Dsupporting

confidence: 53%

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Peptide-Independent Folding and CD8αα Binding by the Nonclassical Class I Molecule, Thymic Leukemia Antigen

Weber¹,

Attinger

Kemball³

et al. 2002

The Journal of Immunology

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The nonclassical class I molecule, thymic leukemia (TL), has been shown to be expressed on intestinal epithelial cells and to interact with CD8+ intraepithelial T lymphocytes. We generated recombinant soluble TL (T18d) H chains in bacteria as inclusion bodies and refolded them with β2-microglobulin in the presence or absence of a random peptide library. Using a mAb, HD168, that recognizes a conformational epitope on native TL molecules, we observed that protein folds efficiently in the absence of peptide. Circular dichroism analysis demonstrated that TL molecules have structural features similar to classical class I molecules. Moreover, thermal denaturation experiments indicated that the melting temperature for peptide-free TL is similar to values reported previously for conventional class I-peptide complexes. Our results also show that CD8αα binding is not dependent on either TL-associated peptide or TL glycosylation.

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Section: Thermal Stability Of T18 Dsupporting

confidence: 53%

“…Far-UV CD spectroscopy has been used to analyze the structure and thermal stability of a variety of classical and nonclassical MHC molecules (17,(35)(36)(37)(38)(39)(40). The far-UV spectra of T18 d are shown in Fig.…”

Section: Thermal Stability Of T18 Dmentioning

confidence: 99%

Peptide-Independent Folding and CD8αα Binding by the Nonclassical Class I Molecule, Thymic Leukemia Antigen

Weber¹,

Attinger

Kemball³

et al. 2002

The Journal of Immunology

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“…This is significant because refolding of recombinant classical class I molecules from purified E. coli inclusion bodies is dependent upon the presence of a peptide ligand. In contrast, nonligand-dependent, nonclassical class I molecules readily refold from inclusion bodies via oxidative refolding conditions (43)(44)(45)(46)(47).…”

Section: Production Of Soluble Hmr1 (Ectodomain) and Its Initial Biocmentioning

confidence: 99%

Biochemical Features of the MHC-Related Protein 1 Consistent with an Immunological Function

Miley

Truscott

et al. 2003

The Journal of Immunology

107

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MHC-related protein (MR)1 is an MHC class I-related molecule encoded on chromosome 1 that is highly conserved among mammals and is more closely related to classical class I molecules than are other nonclassical class I family members. In this report, we show for the first time that both mouse and human MR1 molecules can associate with the peptide-loading complex and can be detected at low levels at the surface of transfected cells. We also report the production of recombinant human MR1 molecules in insect cells using highly supplemented media and provide evidence that the MR1 H chain can assume a folded conformation and is stoichiometrically associated with β2-microglobulin, similar to class I molecules. Cumulatively, these findings demonstrate that surface expression of MR1 is possible but may be limited by a specific ligand or associated molecule.

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“…While phosphorylated small molecules that are intermediates of the microbial mevalonate metabolic pathway ('phosphoantigens') are prominent ligands for the human Vc9Vd2 T cells, such ligands are not recognized by murine cd T cells. Not taking into account such species differences, cd TCRs recognize T10/T22, [30][31][32][33] CD1c, 34,35 major histocompatibility complex class I-related chains A and B (MICA/B), 36 phosphoantigens 37 and ATP synthase-1/apolipoprotein A-1 complex. 38 Striegl and colleagues reported that CD1d is able to bind and present tetraacylated phospholipid cardiolipin to a subset of CL-responsive cd T cells that exist in the spleen and liver of healthy mice.…”

Section: Antigens and Costimulatory Moleculesmentioning

confidence: 99%

Current progress in γδ T-cell biology

Hao

Xia

et al. 2010

Cell Mol Immunol

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T lymphocytes bearing c-and d-chain T-cell receptor heterodimers are named cd T cells. Interestingly, cd and ab T cells share the same progenitors, and they undergo a fate decision in the thymus. Functional differentiation of cd T cells occurs both inside and outside the thymus. Antigen recognition of cd T-cell receptors is very unique, and the responses frequently exhibit innate characteristics. Nevertheless, peripheral cd T cells exert a number of effector and regulatory functions. cd T cells rapidly produce cytokines like interferon (IFN)-c and IL-17 and promote inflammation, partly due to the inherent epigenetic and transcriptional programs, which facilitates a quick and extensive response. Moreover, cd T cells lyse target cells directly, and this is necessary for pathogen or tumor clearance. cd T cells can even serve as regulatory cells, and may contribute to immune suppression. Orchestration of cd T-cell and other immune cell interactions may be critical for host defense and immune regulation. Recently, cd T cells have been used for immunotherapy for infectious diseases and malignancy. In this review, we summarize the abstracts presented at the recent cd T cell Conference held from 19 to 21 May 2010, in Kiel, Germany (please see the website for details: http://www.gammadeltaconference.uni-kiel.de/index.html). Peptide-major histocompatibility complex-specific T-cell responses are known to be achieved by ab T cells, while cd T-cell biology is much less studied. Mice without cd T cells are highly susceptible to diseases and tumor, suggesting an important role of cd T cells in defense. By current technology in cellular and molecular immunology, researchers revealed that cd T cells produce a series of cytokines in pathology, and the effector cd T cells play an indispensable role in pathogen elimination, immune regulation and autoimmunity. As a group of innate immune cells, cd T cells respond rapidly and expand efficiently when stimulated by antigens or cytokines. cd T cells may be a good target for modulation of immune responses in human diseases. In recent years, a lot of researchers focused on the cd T-cell development, function and therapeutic use.

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The Recognition of the Nonclassical Major Histocompatibility Complex (MHC) Class I Molecule, T10, by the γδ T Cell, G8

Cited by 90 publications

References 38 publications

Peptide-Independent Folding and CD8αα Binding by the Nonclassical Class I Molecule, Thymic Leukemia Antigen

Peptide-Independent Folding and CD8αα Binding by the Nonclassical Class I Molecule, Thymic Leukemia Antigen

Biochemical Features of the MHC-Related Protein 1 Consistent with an Immunological Function

Current progress in γδ T-cell biology

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