The recN locus of Escherichia coli K12: molecular analysis and identification of the gene product

Picksley, Steven M.; Morton, Stuart J.; Lloyd, Robert G.

doi:10.1007/bf00425675

Cited by 28 publications

(14 citation statements)

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“…Two possible explanations for the observed incomplete complementation of recO mutant strains by the recO plasmids are as follows: (i) the presence of the recO gene on a (43) and uvrD (35), and the presence of the recO gene on a multicopy plasmid in recB recC sbcA strains causes a recombination defect; or (ii) recO is not expressed well enough from pRDK86 to complement these defects. To investigate the first possibility, the effect of pRDK86 and pBR322 on the repair of UV damage in E. coli AB1157, the parental strain, was investigated (Fig.…”

Section: Resultsmentioning

confidence: 99%

Molecular analysis of the Escherichia coli recO gene

et al. 1989

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The plasmid pLC7-47, which contains lep, rnc, and era, was found to complement the UV-sensitive and recombination-deficient phenotypes caused by the recO1504::Tn5 mutation. Southern blotting analysis demonstrated that pLC7-47 contained a segment of Escherichia coli DNA that covered the region of the E. coli chromosome containing the recO1504::TnS mutation. A combination of deletion mapping and insertional mutagenesis localized the recO-complementing region to an approximately 1-kilobase region of a 1. Genetic analysis of recombination and repair in Escherichia coli has identified 11 genes, recA, recB, recC, recD, recE, recF, recJ, recN, recO, recQ, and riy, whose gene products are required for recombination in E. coli (2,4,12,16,20,24,26,29,31,40,52). Mutations in genes whose products are involved in other aspects of DNA metabolism are also known to affect recombination (46). Historically, E. coli recombination genes have been classified as acting in the RecBC, RecE, or RecF recombination pathway depending on whether they were required for conjugal recombination in the wild-type, recB recC sbcA, or recB recC sbcB strain, respectively (10). The relationship between recombination genes and recombination pathways has not proven to be this simple. Extensive genetic analysis has demonstrated that the gene products required for a particular recombination event depend on the structure of the recombination substrate studied and the genetic background of the cell (10,18,20,27,30,32,34,46; C. A. Luisi-Deluca, S. T. Lovett, and R. D. Kolodner, Genetics, in press). In some cases, a particular recombination pathway even fails to recognize a particular recombination substrate. To understand this apparent diversity of recombination events, it will be necessary to obtain a greater understanding of the biochemical activities of recombination proteins and the mechanism of individual recombination events.The most recently identified E. (oli recombination gene is the recO gene (26). The recO gene product has a broad involvement in different recombination and repair events in E. coli. In wild-type E. col/i strains, the recO gene product is required for recombination of circular plasmids, the repair of UV damage to DNA, and the formation of a portion of the transcribable recombination intermediates that are formed during conjugal recombination (26,30). In lrecB recC shb B mutant strains, the recO mutation behaves like a typical RecF pathway mutation, decreasing the frequency of both conjugal recombination, circular plasmid recombination, linear dimer plasmid recombination, and the repair of DNA damage caused by UV irradiation or mitomycin C (26; * Corresponding author.Luisi-Deluca et al., in press). In recB recC sbcA mutant strains, the recO mutation, like many other RecF pathway mutations, decreases conjugal recombination and the repair of UV-induced DNA damage (Luisi-Deluca et al., in press). The re(O mutation also decreases the frequency of recombination of circular plasmid substrates in recB recC sbcA strains but has no effect on...

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Section: Resultsmentioning

confidence: 99%

Molecular analysis of the Escherichia coli recO gene

et al. 1989

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“…Proteins involved in the RecF pathway of E. coli include RecF, RecO, RecR, RecQ, and RecJ (31,32,58,59). The RecN protein has been assigned to the RecF pathway of E. coli based on a 20-to 30-fold induction of recN in recBC sbcBC strains (38). However, the RecN protein is also required for RecBCD-dependent repair of double-strand breaks which arise as a result of DNA damage (37,41,43,62).…”

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confidence: 99%

“…In E. coli, both conjugational and transductional recombination are severely reduced by a recN mutation in recBC sbcBC mutant backgrounds (29,42), and recN is induced 20-to 30-fold in this same mutant background (38). RecN plays a role in RecBCD-dependent repair of double-strand breaks caused by DNA damage (35,38,42,63), as well as intramolecular recombination of DNA by the RecF pathway (23).…”

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Roles of therecJandrecNGenes in Homologous Recombination and DNA Repair Pathways ofNeisseria gonorrhoeae

2002

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The paradigm of homologous recombination comes from Escherichia coli, where the genes involved have been segregated into pathways. In the human pathogen Neisseria gonorrhoeae (the gonococcus), the pathways of homologous recombination are being delineated. To investigate the roles of the gonococcal recN and recJ genes in the recombination-based processes of the gonococcus, these genes were inactivated in the N. gonorrhoeae strain FA1090. We report that both recN and recJ loss-of-function mutants show decreased DNA repair ability. In addition, the recJ mutant was decreased in pilus-dependent colony morphology variation frequency but not DNA transformation efficiency, while the recN mutant was decreased in DNA transformation efficiency but not pilus-dependent variation frequency. We were able to complement all of these deficiencies by supplying an ectopic functional copy of either recJ or recN at an irrelevant locus. These results describe the role of recJ and recN in the recombination-dependent processes of the gonococcus and further define the pathways of homologous recombination in this organism.

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“…Earlier studies have implicated recN in the repair of interstrand cross-links affected by mitomycin C (Picksley et al, 1985). Data presented in this communication imply that the recN gene may also have roles (albeit limited) in the repair of PUVA-damaged DNA.…”

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confidence: 60%