The Rearing of Microplitis manilae (Hymenoptera: Braconidae) on Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Feed Different Diets

Abstract

A good rearing method is one of the keys in successful biological control practices. This also applied on biological control of Spodoptera litura Fabricius (Lepidoptera: Noctuidae). The objective of this study is to observe the development of Microplitis (Snellanius) manilae (Hymenoptera: Braconidae) on S. litura which cultured in the natural diet (host plants) and the artificial diet. This study was conducted with two treatments which were larvae of S. litura fed with natural diet and artificial diet. Each treatment has 10 individuals as replicates. Each larva was placed in a different petri dish along with the adult of M. manilae. The larvae were exposed to adult parasitoids of M. manilae for 24 hr. Then, the larvae were reared until the parasitoid emerged. The results showed that the highest number of parasitization was found in the hosts which were fed on an artificial diet. Whereas, the highest number of parasitoids emerged were found from the hosts which provided with a natural diet. On both diets, more female parasitoids emerged than males. The difference in nutrient content given to host larvae plays a major role in affecting the number of hosts parasitization, the number of emerged parasitoids and the sex ratio of parasitoids.

The capacity of Trichogramma chilotraeae (Hymenoptera: Trichogrammatidae) to parasitize Spodoptera frugiperda reared with natural diet vs artificial diet vs alternate host

“…An artificial diet was made, according to Sari et al [14]. 1000 mL of distilled water was used to soak 125 grams of red beans for 24 hours.…”

Chromosome-Scale Genome Assembly of the Solitary Parasitoid Wasp Microplitis manilae Ashmead, 1904 (Braconidae: Microgastrinae)

“…Recent studies on M . manilae mainly focused on ecology, such as the effect of different external factors (temperature, host plant, and chilling treatment) on the development, fecundity, parasitism rate, emergence rate, sex ratio, and life span of these organisms ( Qiu et al 2012 , 2013 ; Fang et al 2014 ; Wu et al 2016 ; Sari 2019 ; Xing et al 2022 ). The growth and development of M .…”