The real factor for polypeptide elongation in Dictyostelium cells is EF-2B, not EF-2A

Yoshino, Tomoko; Maeda, Yasuo; Amagai, Aiko

doi:10.1016/j.bbrc.2007.05.155

Cited by 2 publications

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“…2003). This suggested the presence of another molecule capable of compensating for EF‐2A function, and recently the real factor for polypeptide elongation in Dictyostelium cells was found to be EF‐2B, using ef‐2b knockdown cells prepared by the RNAi method (Yoshino et al. 2007).…”

Section: Discussionmentioning

confidence: 99%

Novel functions of ribosomal protein S6 in growth and differentiation of Dictyostelium cells

et al. 2009

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We have previously shown that in Dictyostelium cells a 32 kDa protein is rapidly and completely dephosphorylated in response to starvation that is essential for the initiation of differentiation (Akiyama & Maeda 1992). In the present work, this phosphoprotein was identified as a homologue (Dd-RPS6) of ribosomal protein S6 (RPS6) that is an essential member for protein synthesis. As expected, Dd-RPS6 seems to be absolutely required for cell survival, because we failed to obtain antisense-RNA mediated cells as well as Dd-rps6-null cells by homologous recombination in spite of many trials. In many kinds of cell lines, RPS6 is known to be located in the nucleus and cytosol, but Dd-RPS6 is predominantly located in the cell cortex with cytoskeletons, and in the contractile ring of just-dividing cells. In this connection, the overexpression of Dd-RPS6 greatly impairs cytokinesis during axenic shake-cultures in growth medium, resulting in the formation of multinucleate cells. Much severe impairment of cytokinesis was observed when Dd-RPS6-overexpressing cells (Dd-RPS6 OE cells) were incubated on a living Escherichia coli lawn. The initiation of differentiation triggered by starvation was also delayed in Dd-RPS6 OE cells. In addition, Dd-RPS6 OE cells exhibit defective differentiation into prespore cells and spores during late development. Thus, it is likely that the proper expression of Dd-RPS6 may be of importance for the normal progression of late differentiation as well as for the initiation of differentiation.

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Section: Discussionmentioning

confidence: 99%

Novel functions of ribosomal protein S6 in growth and differentiation of Dictyostelium cells

et al. 2009

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Cell‐cycle checkpoint for transition from cell division to differentiation

Maeda¹

2011

Dev Growth Differ

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In general, growth and differentiation are mutually exclusive, but they are cooperatively regulated during the course of development. Thus, the process of a cell's transition from growth to differentiation is of general importance for the development of organisms, and terminally differentiated cells such as nerve cells never divide. Meanwhile, the growth rate speeds up when cells turn malignant. The cellular slime mold Dictyostelium discoideum grows and multiplies as long as nutrients are supplied, and its differentiation is triggered by starvation. A critical checkpoint (growth ⁄ differentiation transition or GDT point), from which cells start differentiating in response to starvation, has been precisely specified in the cell cycle of D. discoideum Ax-2 cells. Accordingly, integration of GDT point-specific events with starvation-induced events is needed to understand the mechanism regulating GDTs. A variety of intercellular and intracellular signals are involved positively or negatively in the initiation of differentiation, making a series of cross-talks. As was expected from the presence of the GDT point, the cell's positioning in cell masses and subsequent cell-type choices occur depending on the cell's phase in the cell cycle at the onset of starvation. Since novel and multiple functions of mitochondria in various respects of development including the initiation of differentiation have been directly realized in Dictyostelium cells, they are also reviewed in this article.

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The real factor for polypeptide elongation in Dictyostelium cells is EF-2B, not EF-2A

Cited by 2 publications

References 22 publications

Novel functions of ribosomal protein S6 in growth and differentiation of Dictyostelium cells

Novel functions of ribosomal protein S6 in growth and differentiation of Dictyostelium cells

Cell‐cycle checkpoint for transition from cell division to differentiation

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