The Reactions Of Ozone With Organic Compounds

Bailey, Philip S.

doi:10.1021/cr50023a005

Cited by 584 publications

(252 citation statements)

References 307 publications

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“…If the membrane potential were low (A4& = -35 mv) and stable during ozonation, increased efflux is due to a 16-fold increase in Pk, accounting for our measured value. Then subsequently, the rate of the passive influx can be calculated to be 1 and the efflux 15-fold, likewise near our observed changes. This second explanation seems more likely in view of known membrane behavior.…”

Section: Discussionsupporting

confidence: 77%

Ozone Alteration of Membrane Permeability in Chlorella

Heath

Frederick²

1979

Plant Physiol.

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(21,22), and ions (6, 10). Accordingly, the loss of K+ has been shown to be one of the first indications of membrane permeability changes under a variety of stresses (5,17,18,20).The net loss of K+ from ozone-treated Chlorella cells suspended in a Tris buffer has been measured using a cation-specific electrode (6). The electrode method measured only the net loss of K+ at a low external K+ concentration. Thus, the effect of ozone upon the K+ influx and efflux could not be studied separately under physiological conditions. The use of Tris as a buffer induces K+ loss itself, thus making the interpretation of K+ loss difficult (7). For influx measurements, I09 cells were added to a total volume of 5 ml of medium in a stirred and temperature-regulated cuvette (38 C). Label ("6RbCl, New England Nuclear) was added prior to cell addition and subsequent gassing. Either oxygen or ozone in oxygen, produced and measured as previously described (6, 11), was introduced into the agitated solution through a 50-,ul micropipette. At specific times, a 200-jul aliquot of the cell suspension was withdrawn, filtered on a Millipore filter (0.45 ,um), and washed with 5 ml of unlabeled medium. The cell sample on the filter was removed to a drying stand, bleached with I drop of Purex, and placed in a toluene-Triton cocktail for scintillation counting (14).Uptake rates were calculated from the amount of 8'Rb present in the cells at various time intervals, based upon the external specific radioactivity of Rb+ and K+.In experiments during which the initial uptake kinetics were examined, l-ml aliquots from a batch of cells (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)

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Section: Discussionsupporting

confidence: 77%

Ozone Alteration of Membrane Permeability in Chlorella

Heath

Frederick²

1979

Plant Physiol.

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“…The mechanisms of ozone damage to tissues at the molecular level have been studied extensively and are well reviewed (1,11). The oxidation of sulfhydryl moieties to disulfide, sulfenic, or sulfonic groups, the oxidation of unsaturated lipids, and the opening of aromatic ring structures have all been demonstrated in chemical systems.…”

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confidence: 99%

“…The oxidation of several biological molecules by ozone has been demonstrated in chemical systems (1,11) although the relevance of these reactions to in vivo ozone damage is unclear (6). The condensation of amino acid residues by the oxidation of sulfhydryl moieties to disulfide bridges or further oxidation to sulfenic (-SO3H) or sulfonic (-SO4H) groups are feasible, the latter being an irreversible oxidation (11).…”

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confidence: 99%

Inhibition of the K⁺-Stimulated ATPase of the Plasmalemma of Pinto Bean Leaves by Ozone

Dominy¹,

Heath²

1985

Plant Physiol.

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MATERIALS AND METHODSThree varieties of Phaseolus vulgaris which differ in their sensitivity to ozone were examined for changes in some physiological and structural plasma membrane characteristics. Plasma membrane vesicles were prepared from control and ozone-treated (0.2 to 0.5 microliters per liter ozone for 5 hours) leaf tissue, and the (K' + Mg2')-ATPase activity determined and compared. No major changes were observed in the resistant varieties. The sensitive variety showed a severe inhibition of ATPase activity which was largely due to a decrease in the K-stimulated component. This inhibition was completely reversed by the addition of sulfhydryl compounds.Ozone-induced plasma membrane permeability changes may be effected by damage to membrane proteins, perhaps by oxidation of amino acid sulfhydryl groups to disulfide and sulfenic moieties. (15). Ozonation was carried out as described previously (13).Preparation of Cell Fractions. Plasma membrane fractions were prepared from leaf tissue according to slightly modified procedures described elsewhere for root tissue (5,10). Briefly, 10 g of leaf tissue was homogenized for 5 s in 150 ml of isolation medium (50 mM Tris-Mes (pH 6.8), 0.3 M sucrose, 3 mM EDTA, 3 mM DTE, and 1% BSA) in a Polytron. The resulting brei was centrifuged at 3,000g (3 min) and then at 13,000g (15 min) and the pellets discarded. The 13,000g supernatant was then centrifuged at 80,000g (45 min) and the resultant pellet resuspended in 1 ml of isolation medium and placed on top of a 20-33-38% discontinuous sucrose gradient and centrifuged at 80,000g for 2 h. The milky band which appeared at the 33% to 38% sucrose interface was removed. Previous studies have shown this fraction to be enriched in plasma membrane vesicles (5). At all stages of the isolation procedure, the cell fractions were kept at 2°C. All fractions were kept in liquid nitrogen until required.To show that we had a good ATPase preparation from the plasmalemma, we examined the effect of pH and inhibitors on the ATPase activity of our fraction (Fig. 1). This fraction showed maximal stimulation at pH 6.0 to 6.5; increasing or decreasing pH produced a severe inhibition of activity. Mitochondrial and acid phosphatase inhibitors, azide and molybdate, respectively, had little or no effect upon the ATPase activity. However, orthovanadate, a plasma membrane ATPase inhibitor (5), completely abolished the stimulation at pH 6.0, producing a relatively flat pH profile with an activity of approximately 25% of the maximum value. These results are similar to those reported elsewhere for corn plasma membranes (5,13

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“…Alkenes are known to be prone to reactions with ozone via cleavage of their double-bonds [11]. It was thus chosen to use 1-hexebe as an alkene of suitable size and volatility as model fuel for this work.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

1-hexene autoignition control by prior reaction with ozone

Schönborn

Hellier

Ladommatos

et al. 2016

Fuel Processing Technology

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The autoignition timing of 1-hexene was controlled in a Homogeneous Charge Compression Ignition (HCCI) engine by reacting the fuel with ozone-containing air prior to its combustion. The experiments were conducted in a single cylinder research engine instrumented with a cylinder pressure sensor. The fuel was chemically characterised using Nuclear Magnetic Resonance (NMR) spectroscopy before and after its reaction with ozone. The NMR analyses showed that this preliminary reaction produced several oxygenated products within the fuel, and was likely to have resulted in the formation of ozonide molecules having a peroxidic structure with several oxygen molecules in series. To understand how this would affect the ignition reactions, the ignition process was modelled numerically using a single-zone chemical kinetic reactor model. The modelling suggested that peroxide molecules decomposed during the compression of the reactants in the engine and advanced ignition timing by promoting early decomposition of the fuel through the formation of radicals.

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The Reactions Of Ozone With Organic Compounds

Cited by 584 publications

References 307 publications

Ozone Alteration of Membrane Permeability in Chlorella

Ozone Alteration of Membrane Permeability in Chlorella

Inhibition of the K⁺-Stimulated ATPase of the Plasmalemma of Pinto Bean Leaves by Ozone

1-hexene autoignition control by prior reaction with ozone

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The Reactions Of Ozone With Organic Compounds

Cited by 584 publications

References 307 publications

Ozone Alteration of Membrane Permeability in Chlorella

Ozone Alteration of Membrane Permeability in Chlorella

Inhibition of the K+-Stimulated ATPase of the Plasmalemma of Pinto Bean Leaves by Ozone

1-hexene autoignition control by prior reaction with ozone

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Inhibition of the K⁺-Stimulated ATPase of the Plasmalemma of Pinto Bean Leaves by Ozone