The RBE of Fast Neutrons for in Vitro Inactivation of Human Tumour Cells Determined by the Ratio of Mean Inactivation Doses

Courdi, A.; Brassart, N.; Hérault, J.; Mari, Danielle; Chauvel, P

doi:10.3109/02841869609098507

Cited by 8 publications

(2 citation statements)

References 31 publications

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“…Besides calculation of SF2 values (the surviving fraction of cells irradiated with a dose of 2 Gy), radiosensitivities were also expressed in terms of the mean inactivation dose (MID). This parameter quantifies radiosensitivity in a one-dimensional parameter with units of dose (Gy) (Courdi et al, 1996). The mean inactivation dose is proportional to the area under the survival curve.…”

Section: Crystal Violet Cell Proliferation Assaymentioning

confidence: 99%

Comparison of the colony formation and crystal violet cell proliferation assays to determine cellular radiosensitivity in a repair-deficient MCF10A cell line

Vandersickel

Slabbert

Thierens

et al. 2011

Radiation Measurements

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Section: Crystal Violet Cell Proliferation Assaymentioning

confidence: 99%

Comparison of the colony formation and crystal violet cell proliferation assays to determine cellular radiosensitivity in a repair-deficient MCF10A cell line

Vandersickel

Slabbert

Thierens

et al. 2011

Radiation Measurements

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“…These include iso-effect doses, 2,4,11,12 initial slopes of survival curves, 11 and mean inactivation doses. 3,6,7,13 The latter approach is often preferred because it is representative of the entire cell population, minimizes interexperimental variations, and distinguishes cell types according to their intrinsic radiosensitivity. 14 RBEs derived from the initial slopes of survival curves (␣-coefficients) are more indicative of the low dose response (up to 2 Gy), which is clinically relevant, especially for dose fractionation.…”

mentioning

confidence: 99%

Micronucleus Response of Human Glioblastoma and Neuroblastoma Cells Toward Low-LET Photon and High-LET p(66)/Be Neutron Irradiation

Akudugu

Slabbert

Roos

et al. 2003

American Journal of Clinical Oncology

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The identification of photon resistant tumors that are sensitive to neutrons is still an unresolved problem, and no radiobiological criteria have been developed that could help the selection of patients for neutron therapy. The micronucleus (MN) assay has been evaluated for this purpose in a panel of human glioblastoma and neuroblastoma cell lines spanning a wide range of photon sensitivities defined by mean inactivation doses ([Latin capital letter D with macron above][gamma]) of 1.25-3.21 Gy. We show that the relative biologic effectiveness (RBE) of the p(66)/Be neutrons is significantly correlated with inherent photon sensitivity (r = 0.89, p < 0.01), indicating that the panel of cell lines used is suitable to study the differential biologic response to neutrons and photons. We find that p(66)/Be neutrons are 1.43 to 5.29 times more effective per unit dose in inducing micronuclei than 60Co [gamma]-rays. Surprisingly, cells that are inherently photon resistant tend to show a higher yield of micronuclei following exposure to either photons or neutrons, but no significant correlation could be demonstrated. However, RBE values based on micronucleus yield were found to strongly correlate with RBE values derived from cell survival data (r = 0.91, p < 0.01). It is concluded that although micronucleus yield does not reflect intrinsic sensitivity to either photons or neutrons, the strong correlation between RBE calculated from micronucleus formation and RBE derived from cell survival demonstrates that the micronucleus endpoint has a potential for detecting photon resistant cells that show increased sensitivity to neutrons.

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Neutron and photon clonogenic survival curves of two chemotherapy resistant human intermediate-grade non-Hodgkin lymphoma cell lines

Aref

Yudelev

Mohammad

et al. 1999

International Journal of Radiation Oncology*Biology*Physics

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The RBE of Fast Neutrons for in Vitro Inactivation of Human Tumour Cells Determined by the Ratio of Mean Inactivation Doses

Cited by 8 publications

References 31 publications

Comparison of the colony formation and crystal violet cell proliferation assays to determine cellular radiosensitivity in a repair-deficient MCF10A cell line

Comparison of the colony formation and crystal violet cell proliferation assays to determine cellular radiosensitivity in a repair-deficient MCF10A cell line

Micronucleus Response of Human Glioblastoma and Neuroblastoma Cells Toward Low-LET Photon and High-LET p(66)/Be Neutron Irradiation

Neutron and photon clonogenic survival curves of two chemotherapy resistant human intermediate-grade non-Hodgkin lymphoma cell lines

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