The Ratio of Treg/Th17 Cells Correlates with the Disease Activity of Primary Immune Thrombocytopenia

Ji, Lili; Zhan, Yanxia; Hua, Fanli; Li, Feng; Zou, Shanhua; Wang, Weiguang; Song, Dongli; Min, Zhihui; Chen, Hao; Cheng, Yunfeng

doi:10.1371/journal.pone.0050909

Cited by 97 publications

(83 citation statements)

References 30 publications

(36 reference statements)

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“…As seen in Fig. 2, RORct expression was significantly higher in active ITP patients (05.02 ± 1.03) than that in healthy controls (0.99 ± 0.22) (P < 0.01), suggesting the abnormal Th17 cells in ITP, consistent with the previously studies showing the association of Th17 differentiation with the pathogenesis of ITP [9][10][11]. However, its level was significantly lower in patients in remission (1.37 ± 0.40) than that in active patients (P < 0.01) without significant difference to controls (P > 0.05).…”

Section: Expression Of Rorct and Th17 Cells In Itp Patients And Controlssupporting

confidence: 89%

“…T helper cells (Th)1 and Th2 cells, two T cell subsets, are important for autoimmune reactivity and imbalance of Th1/Th2 has been reported to be associated with ITP [5,8]. However, in recent years, apart from Th1/Th2, another novel subset of Th cells, IL-17 secreting Th-cells (Th17) and associated cytokines (IL-17) are also identified to be implicated in the pathogenesis of ITP [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Increased RUNX1 expression in patients with immune thrombocytopenia

Liu

Zhu

et al. 2016

Human Immunology

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Immune thrombocytopenia (ITP) is a heterogeneous autoimmune disease, characterized by dysregulation of cellular immunity. Th17 and associated IL-17 were involved in the pathogenesis of ITP. Runt-related transcription factor 1 (RUNX1), a member of the runt domain-containing family of transcription factors, is required for Th17 differentiation. Whether RUNX1 was involved in the pathogenesis of ITP remains poorly understood. In this study, 30 active ITP patients, 20 ITP in remission and 20 age and gender matched healthy controls were included. Peripheral blood mononuclear cells (PBMCs) were isolated to measure mRNA level of RUNX1 and retinoic acid receptor-related orphan receptor-γt (RORγt) by quantitative real-time PCR and Th17 cells by flow cytometry. Meanwhile, plasma was extracted for measurement of IL-17 level by ELISA. Our results showed a significantly higher expression of RUNX1, RORγt, Th17 cells and plasma level of IL-17 in active ITP patients than that in healthy controls. No differences of expression of RUNX1, RORγt and Th17 cells were observed between remission patients and controls. Furthermore, a significantly positive correlation of RUNX1 with RORγt was found in active ITP patients. In conclusion, RUNX1 was associated with the pathogenesis of ITP possibly through regulation of Th17 cell differentiation and therapeutically targeting it might be a novel approach in ITP treatment.

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Section: Expression Of Rorct and Th17 Cells In Itp Patients And Controlssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Increased RUNX1 expression in patients with immune thrombocytopenia

Liu

Zhu

et al. 2016

Human Immunology

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“…Third, elevated Th2 cytokines (IL-4, IL-10) have been observed in patients with ITP (24). Fourth, decreased regulatory T (Treg) cells exhibiting impaired function have been observed in ITP patients (25)(26)(27)(28)(29)(30)(31). The dysfunction of Treg cells plays a role in both autoimmune disease and allergic diseases (32)(33)(34)(35).…”

Section: Discussionmentioning

confidence: 99%

Association of primary immune thrombocytopenia and common allergic diseases among children

et al. 2015

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Background:Growing evidence has revealed a link between autoimmune and allergic diseases. However, few studies have assessed the relationship between allergic diseases and primary immune thrombocytopenia (ITP), an autoimmune disease frequently occurring in children. This population-based case-control study investigated the association between common allergic diseases and the subsequent risk of developing ITP during childhood. Methods: This study investigated 1,203 children younger than 18 y of age who were diagnosed with ITP between 1998 and 2008, as well as 4,812 frequency-matched controls. The odds ratios of the association between ITP and preexisting allergic diseases were calculated. results: Children with every type of allergic disease examined in this study (except asthma) exhibited an increased risk of developing ITP; the lowest adjusted odds ratio (aOR) was 1.39 for allergic conjunctivitis (95% confidence interval (CI) = 1.09-1.79), whereas the greatest aOR was 1.84 for allergic rhinitis (95% CI = 1.49-2.27). The aORs increased with the number of concurrent allergic diseases to 2.89 (95% CI = 1.98-4.22) for children with at least three allergic diseases. conclusion: Children with atopic diathesis have a greater risk of subsequently developing ITP. The fundamental determinants of this relationship warrant further study.

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“…Antiplatelet antibodies mediate rapid clearance from the circulation in large part via the reticuloendothelial (monocytic phagocytic) system [11]. In addition, cellular immunity is perturbed and T cell and cytokine profiles are significantly shifted towards a type 1 and Th17 proinflammatory immune response [12]. The precise mechanism of the immune dysfunction, however, is generally not known.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Status and Oxidative Stress in Patients with Chronic ITP

et al. 2013

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The aim of this study was to establish the antioxidant status and oxidative stress in adult patients with chronic idiopathic thrombocytopenic purpura (ITP). Eighty-four patients diagnosed with chronic ITP were studied. Fiftyeight age-matched healthy subjects were selected as controls. Serum nitrogen monoxide ( NO), oxidized glutathione (GSSG), malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), superoxide dismutase (SOD), hydrogen peroxide enzyme (CAT), glutathione peroxidase (GSH-Px), glutathione (GSH) were evaluated by enzyme-linked immunosorbent assay (ELISA). It was found that serum SOD, CAT, GSH-Px, GSH, TAS levels were significantly lower in patients with chronic ITP than controls (all P < 0.05), while serum NO, GSSG, MDA, TOS values were significantly higher (P < 0.05). The number of platelet showed a negative correlation with NO, GSSG, MDA, TOS, respectively,while platelet number showed a positive correlation with SOD, CAT, GSH-Px, GSH, TAS. These findings suggested that oxidants were increased and antioxidants were decreased in patients with chronic ITP, these may be prominent factors in destructing the platelet membrane. The scavenging of oxygen radical provides a theoretical basis for the treatment of ITP patients.

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The Ratio of Treg/Th17 Cells Correlates with the Disease Activity of Primary Immune Thrombocytopenia

Cited by 97 publications

References 30 publications

Increased RUNX1 expression in patients with immune thrombocytopenia

Increased RUNX1 expression in patients with immune thrombocytopenia

Association of primary immune thrombocytopenia and common allergic diseases among children

Antioxidant Status and Oxidative Stress in Patients with Chronic ITP

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