The ratio of growth differentiation factor 9: Bone morphogenetic protein 15 mRNA expression is tightly co-regulated and differs between species over a wide range of ovulation rates

Crawford, Janet L.; McNatty, Kenneth P.

doi:10.1016/j.mce.2011.09.033

Cited by 95 publications

(134 citation statements)

References 37 publications

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“…Nevertheless, the results are consistent with previous reports based on expression levels in individual oocytecumulus cell complexes and DO , Crawford & McNatty 2012.…”

Section: Discussionsupporting

confidence: 93%

“…the number of follicles ovulated during an oestrous or menstrual cycle) (Dong et al 1996, Galloway et al 2000, Di Pasquale et al 2004, McNatty et al 2005c, Otsuka et al 2011. The evidence in sheep and rodents, as in other species such as pig, deer and cow, is that GDF9 and BMP15 mRNA are expressed in oocytes and not in cumulus cells , Crawford & McNatty 2012. Moreover, GDF9 is expressed in oocytes from the primordial stage in both sheep and rodents whereas BMP15 is expressed only from the primary stage of growth.…”

Section: Introductionmentioning

confidence: 99%

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Effects of species differences on oocyte regulation of granulosa cell function

et al. 2012

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The aims were to investigate whether oocyte-secreted growth factors from a high (i.e. rat) and low (i.e. sheep) ovulation rate species could stimulate 3 H-thymidine incorporation in granulosa cells (GC) from antral follicles from the same or across species. Denuded oocytes (DO) were co-incubated with GC with or without specific antibodies to growth differentiating factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15). Co-incubations of DO-GC from the same or across species significantly increased thymidine incorporation in GC with increasing numbers of DO. GDF9 immuno-neutralisation reduced thymidine incorporation in rat GC co-incubated with either rat or ovine DO and in ovine GC co-incubated with ovine or rat DO. BMP15 immuno-neutralisation only reduced thymidine incorporation when ovine DO were co-incubated with either ovine or rat GC. Western blotting of oocytes co-incubated with GC identified GDF9 and BMP15 proteins for sheep and GDF9 protein for rats in oocyte lysates and incubation media. With respect to rat BMP15, a promature protein was identified in the oocyte lysate but not in media. Expression levels of GDF9 relative to BMP15 mRNA in DO co-incubated with GC were highly correlated (R 2 Z0.99) within both species. However, the expression ratios were markedly different for the rat and sheep (4.3 vs 1.0 respectively). We conclude that during follicular development, rat oocytes secrete little, if any, BMP15 and that GDF9 without BMP15 can stimulate proliferation of rat and ovine GC. In contrast, ovine oocytes secrete both BMP15 and GDF9, and both were found to stimulate proliferation in ovine and rat GC.

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“…Nevertheless, the results are consistent with previous reports based on expression levels in individual oocytecumulus cell complexes and DO , Crawford & McNatty 2012.…”

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Effects of species differences on oocyte regulation of granulosa cell function

et al. 2012

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“…Information on these aspects is reported extensively elsewhere , Gilchrist et al 2008, Otsuka et al 2011, Paulini & Melo 2011. However, it is important to note that mRNA encoding GDF9 and BMP15 are tightly co-regulated in multiple species (Crawford & McNatty 2012) and GDF9 and BMP15 have synergistic affects at the level of the cell (McNatty et al 2005b(McNatty et al , 2005c. Thus, when examining the physiology of these molecules, the cooperative nature of these molecules is a very important consideration.…”

Section: Mechanisms Of Action Of Gdf9 and Bmp15 And The Involvement Omentioning

confidence: 99%

Using sheep lines with mutations in single genes to better understand ovarian function

Juengel¹,

Davis²,

McNatty³

2013

Reproduction

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Livestock populations have been subjected to strong selection pressure to improve reproductive success, and this has led to the identification of lines of animals with increased fecundity. These animals provide a rich biological resource for discovery of genes and regulatory mechanisms that underpin improved reproductive success. To date, three genes, all related to the transforming growth factor b pathway, have been identified as having mutations that lead to alterations in ovulation in sheep. In addition, several other sheep lines have been identified with putative mutations in single genes with major effects on ovulation rate. This review is focused on the identification of the mutations affecting ovulation rate and how these discoveries have provided new insights into control of ovarian function.Reproduction (2013) 146 R111-R123

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“…The participation of BMP-15 and GDF-9 in the pathogenesis of follicular cysts in pigs has been suggested Błaszczyk, 2014, 2016). In addition, these factors, depending on the stage of folliculogenesis, can either strengthen or weaken the influence of gonadotropins on the ovarian follicle (Knight and Glister, 2006;Crawford et al, 2012). Also, the interactive effect of thyroid hormones and gonadotropins on the formation of follicular cysts has been shown in gilts (Fitko et al, 1995(Fitko et al, , 1996.…”

Section: Discussionmentioning

confidence: 99%

“…It is supposed that BMP-15 and GDF-9 are involved in hormonal regulation of the hypothalamic-pituitary-ovary axis (Paulini and Melo, 2011). These factors, depending on the stage of follicular development, may increase or weaken the influence of gonadotropins on the ovarian follicle (Knight and Glister, 2006;Crawford and McNatty, 2012). In addition, they influence the proliferation and differentiation of somatic cells of the follicle, steroidogenesis, deposition of the extracellular matrix, ovulation, and luteinization (Su et al, 2008;Orisaka et al, 2009;Peng et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The relationships between transforming growth factors <i>β</i> and free thyroxine and progesterone in the ovarian cysts, preovulatory follicles, and the serum of sows

Stankiewicz

2017

Arch. Anim. Breed.

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Abstract. The aim of the study was to determine the relationships between bone morphogenetic protein 15 (BMP-15) and growth differentiation factor 9 (GDF-9) concentrations and free thyroxine (FT 4 ) and progesterone (P 4 ) concentrations in follicular cysts, preovulatory follicles, and the serum of sows (cyst-bearing (n = 26) and non-cyst-bearing (n = 26)). FT 4 and P 4 concentrations were higher in the cystic fluid than in the fluid of preovulatory follicles (p < 0.01 and p < 0.05 respectively). BMP-15 and GDF-9 concentrations were higher in the serum of cyst-bearing sows than non-cyst-bearing sows (p < 0.05) and higher in the cystic fluid than in the follicular fluid (p < 0.05). In the cysts and preovulatory follicles, GDF-9 concentration was higher than in serum (p < 0.01). FT 4 concentration in the serum of cystic sows was correlated with BMP-15 (r = 0.50, p < 0.05) and GDF-9 (r = 0.62, p < 0.01) concentrations in serum. In the serum of non-cyst-bearing sows, a positive correlation between P 4 concentration and BMP-15 concentration (r = 0.60, p < 0.01) was detected.These data will help provide insight into the role of BMP-15, GDF-9, FT 4 , and P 4 during cyst formation in sows.

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The ratio of growth differentiation factor 9: Bone morphogenetic protein 15 mRNA expression is tightly co-regulated and differs between species over a wide range of ovulation rates

Cited by 95 publications

References 37 publications

Effects of species differences on oocyte regulation of granulosa cell function

Effects of species differences on oocyte regulation of granulosa cell function

Using sheep lines with mutations in single genes to better understand ovarian function

The relationships between transforming growth factors <i>β</i> and free thyroxine and progesterone in the ovarian cysts, preovulatory follicles, and the serum of sows

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The ratio of growth differentiation factor 9: Bone morphogenetic protein 15 mRNA expression is tightly co-regulated and differs between species over a wide range of ovulation rates

Cited by 95 publications

References 37 publications

Effects of species differences on oocyte regulation of granulosa cell function

Effects of species differences on oocyte regulation of granulosa cell function

Using sheep lines with mutations in single genes to better understand ovarian function

The relationships between transforming growth factors &lt;i&gt;β&lt;/i&gt; and free thyroxine and progesterone in the ovarian cysts, preovulatory follicles, and the serum of sows

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The relationships between transforming growth factors <i>β</i> and free thyroxine and progesterone in the ovarian cysts, preovulatory follicles, and the serum of sows