The Rate of Success of the Accelerated Solvent Extraction (Ase) of Fat and Organochlorine Pesticides from Dried Fish Meat Samples

Abstract

The replacement of conventional sample preparation techniques with newer techniques which are automated, faster and more eco-friendly, is nowadays desired in every analytical laboratory. One of the techniques with the attributes mentioned above is the Accelerated Solvent Extraction. In order to evaluate how successful this method is for the extraction of fat and organochlorine pesticides (OCPs) from dried fish meat samples, we have tested two series of diverse fish using Dionex™ 350 ASE provided by Thermo Scientific™ (Germany). For a more interesting approach, we added to our investigation 7 polychlorinated biphenyl (PCBs), 3 thricholorobenzenes, 2 tetrachlorobenzenes, 1 pentachlorobenzenes and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The study focused on comparing the recoveries of these analytes from different fish samples, after replacing the conventional reference method of the laboratory with ASE. The ASE parameters tested were previously used for the extraction of fat and polybrominated diphenyl ethers (PBDE) from fish samples: temperature: 120 ° C; static time: 5 min; number of cycles: 3; flushing volume: 25%; rinse with nitrogen: 90 s; solvent: cyclohexane/ethyl acetate (ratio 1:1).The ASE method provided similar and in some cases better results when compared to the standard reference method, more rapidly, eco-friendly and safer. Any high or low recoveries of the analytes taken into study are attributed to random or systematic errors during the Clean-up step of the extracts and the quantification with Gas Chromatography coupled with Tandem Mass-Spectrometry (GC MS/MS).

Methodologies and Results Concerning the Determination of Ocs And Pcbs From Fish Meat

“…Before extracting, the samples of fish must be treated to remove most or all of the water. There are several ways to achieve this, but one of the most efficient ways is freeze-drying (Botaro et al, 2011;Cioca et al, 2017a, Santhi et al, 2012. Choosing a solvent that matches the chemical profile of the studied analytes and setting convenient parameters such first extractions used in the determination of OC and PCB residues from fish.…”