“…The data summarized in table 1 show that there was a significant difference in the degree of encystation of the two strains, particularly from inocula above 5,000 amebae (P < .005).3 In an effort to account for the differences that were occurring, some observations made on the pH showed that the initial pH of the medium was 7.2 and that throughout the incubation period the range for both strains was 6.8 to 7.1 An attempt was also made to determine the rate of multiplication, as a means of explaining the results, since Chang (1942) had linked the process of encystation, at least in part, with an accelerated rate of growth. The calculation based on the method of Brackett and Bliznick (1947) could only be considered as an approximate means of comparison, since neither mortality nor rate of encystation could be taken into account. By this method the rate of growth is expressed as the number of binary divisions per day, and the differences between the two strains are shown in table 1.…”