The rate of mitotic division in regenerating epithelium in the dog

Winstanley, E. W.

doi:10.1016/s0034-5288(18)33601-4

Cited by 2 publications

(2 citation statements)

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“…These phenomena result in a thickened and hyperplastic neoepithelium as previously reported in animal models Krawczyk, 1971;Winstanley, 1975a, b) and in human volunteers . Furthermore, our results support previous studies demonstrating a delayed and transient increase in proliferative activity at the wound margin beginning after migration has initiated (Winstanley, 1975b;Garlick and Taichman, 1994;Laplante et al, 2001).…”

Section: Discussionsupporting

confidence: 92%

“…As previously described in other in vitro and in vivo wound-healing models (Matoltsy and Viziam, 1970;Winstanley, 1975b;Garlick and Taichman, 1994;Laplante et al, 2001;Falanga et al, 2002), a burst of proliferative activity during re-epithelialization occurred in the skin-humanized mice. The burst occurred 1-3 d after wounding, during the process of covering of the wound floor.…”

Section: Discussionsupporting

confidence: 68%

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An In Vivo Model of Wound Healing in Genetically Modified Skin-Humanized Mice

Escámez

García

Larcher

et al. 2004

Journal of Investigative Dermatology

109

100

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Cutaneous wound-healing disorders are a major health problem that requires the development of innovative treatments. Whithin this context, the search for reliable human wound-healing models that allow us to address both mechanistic and therapeutic matters is warranted. In this study, we have developed a novel invivo wound-healing model in a genetically modified human context. Our model is based on the regeneration of human skin on the back of nude mice by transplantation of a cultured bioengineered skin equivalent previously designed in our laboratory. In this setting, human keratinocytes in the epidermal compartment were genetically modified with a retroviral vector encoding the enhanced green fluorescent protein (EGFP). After stable engraftment of the EGFP skin was achieved (9-12 wk after grafting), a small circular full thickness wound was performed on this mature human skin. A wide variety of parameters involved in wound healing were monitored, including tissue architecture, cell proliferation, epidermal differentiation, dermal remodelling, and basement membrane regeneration. Wounded gene-targeted skin-humanized mice re-capitulated native skin wound-healing features. In addition, when keratinocyte growth factor (KGF), a growth factor that has been shown to improve wound healing, was added to wounds during 3 d, the re-epithelialization was significantly accelerated. The present wound-healing model system provides a suitable in vivo tool to test gene transfer strategies for human skin repair. It also serves as a complementary platform for studies in genetically modified mice and as a model to evaluate pharmaceutical therapeutic approaches for impaired wound healing.

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Section: Discussionsupporting

confidence: 92%