The rate of gas exchange between blood cells and serum

Dirken, M. N. J.; Mook, H. W.

doi:10.1113/jphysiol.1931.sp002816

Cited by 71 publications

(12 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The alternative is that the Cl--HCO exchange changes the membrane permeability to 02. Unfortunately, this begs the question because the data in this paper require that the Ch--HCO exchange process itself, which investigators have shown to be slow (Dirken & Mook, 1931;Roughton & Rupp, 1958) (Perutz, 1963) or the molecular re-arrangement of haemoglobin due to the Bohr shift (Benesch, Ranney, Benesch & Smith, 1961) causes enough stirring within the cell to speed up the C1--HCO exchange which seems to be the rate-limiting factor in the Bohr shift. The importance of this event, even if it could be proved to exist, depends on the relative resistance of the membrane and the cell interior to diffusion.…”

Section: Discussionmentioning

confidence: 99%

“…The results, however, are only of qualitative value since the experiment consisted of bubbling nitrogen through a large flask of blood and following the deoxygenation rate over a period of many minutes, which is hundreds of times longer than the time available for exchange in the capillaries. Dirken & Mook (1931) studied the rate of C02 entry into erythrocytes by examining ultrafiltrates of the extraceliular fluid in their rapid reaction apparatus at various times after mixing. They followed the chloride shift and found a half time (tro) of 039 sec at 150 C. More recently Roughton & Rupp (1958) re-studied the chloride shift and found t50 = 0O80 sec normally.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Synergism in the kinetic reactions of O₂ and CO₂ with human red blood cells*

Nakamura¹,

Staub²

1964

The Journal of Physiology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Synergism in the kinetic reactions of O₂ and CO₂ with human red blood cells*

Nakamura¹,

Staub²

1964

The Journal of Physiology

View full text Add to dashboard Cite

“…Chloride and bicarbonate involved in the 'chloride shift' in the red cell can cross the membrane in approximately 1 sec (Dirken & Mook, 1931). But muscle and nerve were long thought to be impermeable by anions (Fenn, 1936;Davson & Danielli, 1952).…”

Section: Discussionmentioning

confidence: 99%

The individual effects of CO₂, bicarbonate and pH on the electrical and mechanical activity of isolated rabbit auricles

Williams¹

1955

The Journal of Physiology

View full text Add to dashboard Cite

The experiments to be described represent an attempt to discover how far hydrogen ions, bicarbonate ions and undissociated carbonic acid molecules individually affect the electrical and mechanical activity of the isolated rabbit auricle, and influence the function of its pace-maker. The frequency and force of auricular contractions can be measured simply enough, but properties of the membrane relevant to its electrical activity are open to investigation in a variety of ways. A direct approach is to measure membrane potentials with capillary KC1

show abstract

“…Experiments with C13s indicated that isotopic equilibrium between both siclded and unsickled S-S ceils and medium was achieved in less than 1 to 2 minutes after adding the isotope to the cell suspension. It has long been known (18) and recently confirmed with CI 8° (19), that chloride penetrates the normal red cell with comparable extreme rapidity. Despite considerable variation, the data (Fig.…”

Section: C) Chloride Distribution Ratio and Approximate Exchange Ratementioning

confidence: 99%

The Effects of Sickling on Ion Transport

Tosteson

Carlsen

Dunham

1955

The Journal of General Physiology

137

View full text Add to dashboard Cite

The conversion of red cells of patients with sickle cell anemia (S-S) from biconcave disk to sickle shape by removal of oxygen was found to increase the fraction of medium trapped in cells packed by centrifugation from 0.036 (S.E. 0.003) to 0.106 (S.E. 0.004). The fraction of water in the cells (corrected for trapped medium) was not affected by this shape transformation. Cation transport, however, was changed profoundly. S-S cells incubated in N2 rather than O2 showed net K loss with acceleration of both influx and outflux. That this change in K transport was due to the process of sickling was indicated by (1) the persistence of the effect in the absence of plasma, (2) the absence of the effect in hypoxic S-S cells in which sickling was inhibited by alkali or carbon monoxide, (3) the reversal of the effect when sickling was reversed by exposure to O2, and (4) the independence of the effect from such potentially important factors as age of the cell population. The acceleration of K transport by sickling is probably mediated by modification of the cell surface rather than the cell interior since concentrated sickle hemoglobin solutions in O2 or N2 did not show selective affinity for K. In molecular terms, the effect of sickling on K transport can be explained by presuming that the shape change (1) opens pathways for the free diffusion of K, and (2) accelerates K transport by a non-diffusion carrier process. The evidence for the former mechanism included (a) dependence of K influx into sickled cells on the concentration of K in the medium, and (b) increase in the total cation content of sickled cells with increasing pH. Observations suggestive of a carrier process included (a) the failure of sickled cell K concentration to become equal to external K concentration even after 48 hours, (b) the deviation of the flux ratio from that characteristic of diffusion, and (c) the dependence of K influx on glycolysis.

show abstract

The rate of gas exchange between blood cells and serum

Cited by 71 publications

References 4 publications

Synergism in the kinetic reactions of O₂ and CO₂ with human red blood cells*

Synergism in the kinetic reactions of O₂ and CO₂ with human red blood cells*

The individual effects of CO₂, bicarbonate and pH on the electrical and mechanical activity of isolated rabbit auricles

The Effects of Sickling on Ion Transport

Contact Info

Product

Resources

About

The rate of gas exchange between blood cells and serum

Cited by 71 publications

References 4 publications

Synergism in the kinetic reactions of O2 and CO2 with human red blood cells*

Synergism in the kinetic reactions of O2 and CO2 with human red blood cells*

The individual effects of CO2, bicarbonate and pH on the electrical and mechanical activity of isolated rabbit auricles

The Effects of Sickling on Ion Transport

Contact Info

Product

Resources

About

Synergism in the kinetic reactions of O₂ and CO₂ with human red blood cells*

Synergism in the kinetic reactions of O₂ and CO₂ with human red blood cells*

The individual effects of CO₂, bicarbonate and pH on the electrical and mechanical activity of isolated rabbit auricles