The rate of change in Ca2+ concentration controls sperm chemotaxis

Álvarez, Luis J.; Dai, Luru; Friedrich, Benjamin M.; Kashikar, Nachiket D.; Gregor, Ingo; Pascal, R.; Kaupp, U. Benjamin

doi:10.1083/jcb.201106096

Cited by 93 publications

(84 citation statements)

References 53 publications

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“…Successful treatment of accessory gland infection was observed to result in rise in concentrations of inorganic phosphate, calcium and magnesium [11]. Furthermore, inorganic phosphate was higher in asthenozoospermic but lower in azoospermic than in normospermic individuals.…”

Section: Introductionmentioning

confidence: 82%

“…But recent information on the subject called these findings to question. For example Alvarez et al, [11] demonstrated that the sperm continued on a straight course even when calcium was abundant and responds to a change in calcium concentration in a mathematical time derived terms. It was further shown that the rate of calcium ion increase dictates how sharply the sperm turns whereas the path of the subsequent runs depend on the steepness of the calcium decline.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the fact that inorganic phosphate was observed to be higher in asthenozoosperma but lower in azoospermic patients than in normospermic men,in other studies underscores the clinical importance of estimation of seminal plasma phosphate. It had also been suggested that estimation of Ca 2+ , Mg 2+ and inorganic phosphate may be of value in the assessment of accessory gland function of the male genitalia [11,17]. Phosphate ion is crucial to the activities of prostatic acid phosphatase (PAP) which has been associated with liquefaction process of semen and adenyl cyclase; the primary regulator of sperm motility [18].…”

Section: Discussionmentioning

confidence: 99%

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Seminal Plasma pH, Inorganic Phosphate, Total and Ionized Calcium Concentrations In The Assessment of Human Spermatozoa Function

Banjoko

Adeseolu

2013

JCDR

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Introduction: Fertilization in humans is dependent on viability of the male spermatozoa among other factors and there have been conflicting reports on the role of pH, calcium and phosphate concentrations in sperm function. This study therefore aimed to investigate seminal plasma pH, inorganic phosphate, total and ionized calcium concentrations relative to spermatozoa function. Material and Methods: Seminal plasma concentrations of pH, total calcium, ionized calcium (Ca ++ ); inorganic phosphate, motility and spermatozoa count were determined in 80 males by standard methods.Results: Forty-nine of the subjects had normal spermatozoa motility (> 60%) and 31 had hypomotility (< 60%). The hypomotility group exhibited lower calcium ion (Ca 2 +) concentrations; 0.19+0.01mmol/L compared with normal motility group; 0.24+0.01mmol/L (p<0.001) the latter also had significantly higher inorganic phosphate; 7.83+1.27 while the former had 5.64+1.62mmol/L (p= 0.004). The mean spermatozoa counts for hypomotility and normal motility group were 42.0 ± 13 x 106 , 72.35 + 20 x 106 respectively (p< 0.001). No significant differences were observed in pH, volume of ejaculate and total calcium concentration between the hypomotility and normal motility groups The mean concentrations of pH were 7.51 ± 0.02 and 7.54 ± 0.03 respectively (p= 0.21) and total calcium; 3.10 ± 0.12 and 3.36 ± 0.14mmol/L respectively (p= 0.16 ). There was a significant difference in percentage of abnormal forms in both groups with hypomotile group having 36% compared to mormal motility group with 5% (p< 0.05). Conclusion:Correlations were observed between seminal concentrations of calcium ions, inorganic phosphate, spermatozoa count and motility but not with total calcium concentrations and pH and therefore should be considered in understanding male infertility and preparation of media for sperm preservation for in vitro fertilization.

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Section: Introductionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Seminal Plasma pH, Inorganic Phosphate, Total and Ionized Calcium Concentrations In The Assessment of Human Spermatozoa Function

Banjoko

Adeseolu

2013

JCDR

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“…For all data, error bars represent mean ± SEM with n = 4-6. Statistical comparisons according to Student's unpaired t test indicated * P < 0.05; ** P < 0.01; *** P < 0.001 versus spermatozoa incubated with 0.1 % DMSO + 10 ng μl −1 of rhZP3 chemotaxis have been reported (Alvarez et al 2012 ;Guerrero et al 2013 ;Ingermann et al 2008 ;Wood et al 2003 ;Wood et al 2007 ).…”

Section: Ca 2+ -Activated CL − Channels (Caccs)mentioning

confidence: 99%

Cl− Channels and Transporters in Sperm Physiology

Treviño

Orta

Figueiras-Fierro

et al. 2014

Sexual Reproduction in Animals and Plants

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Spermatozoa must decode environmental and cellular cues to succeed in fertilization, and this process relies heavily on ion channels. New observations bring to light the relevant participation of Cl − channels and anion transporters in some of the main sperm functions. Here we review the evidence that indicates the participation of Cl − channels in motility, maturation, and the acrosome reaction (AR), and what is known about their molecular identity and regulation. Our better understanding of sperm anion transport will yield tools to handle some infertility problems, improve animal breeding and preserve biodiversity, and develop selective and secure male contraceptives.

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“…SAPs initiate their physiological action on spermatozoa through an increase in cyclic guanosine monophosphate (cGMP) levels (Garbers, 1989;Kaupp et al, 2003), which induces membrane potential (V m ) changes (Galindo et al, 2000;Strünker et al, 2006), leading to increases in intracellular pH (pH i ) and Ca 2+ concentrations ([Ca 2+ ] i ) (Nishigaki et al, 2001;Seifert et al, 2015). Changes in [Ca 2+ ] i in particular play a fundamental role in the regulation of the shape of flagellar beating (Böhmer et al, 2005;Wood et al, 2005), namely, in the process of sperm chemotaxis (Alvarez et al, 2012;Guerrero et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters

Arcos-Hernández

Romero

Sánchez-Guevara

et al. 2016

Journal of Experimental Biology

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Long-range cellular communication between the sperm and egg is critical for external fertilization. Sperm-activating peptides (SAPs) are diffusible components of the outer layer of eggs in echinoderms, and function as chemoattractants for spermatozoa. The decapeptide named speract is the best-characterized sea urchin SAP. Biochemical and physiological actions of speract have been studied with purified or chemically synthesized peptides. In this work, we prepared recombinant speract fused to a fluorescent protein (FP; FP-speract) using three color variants: a cyan (eCFP), a yellow (mVenus) and a large Stokes shift yellow (mAmetrine) FP. Although these fluorescence tags are 20 times larger than speract, competitive binding experiments using mAmetrine-speract revealed that this FPsperact has binding affinity to the receptor that is comparable (7.6-fold less) to that of non-labeled speract. Indeed, 10 nmol l −1 eCFPsperact induces physiological sperm responses such as membrane potential changes and increases in intracellular pH and Ca 2+ concentrations similar to those triggered by 10 nmol l −1 speract. Furthermore, FP-speract maintains its fluorescence upon binding to its receptor. Using this property, we performed fluorescence resonance energy transfer (FRET) measurements with eCFP-speract and mVenus-speract as probes and obtained a positive FRET signal upon binding to the receptor, which suggests that the speract receptor exists as an oligomer, at least as a dimer, or alternatively that a single speract receptor protein possesses multiple binding sites. This property could partially account for the positive and/or negative cooperative binding of speract to the receptor.

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The rate of change in Ca2+ concentration controls sperm chemotaxis

Abstract: Sperm navigate in a chemoattractant gradient by translating changes in intracellular calcium concentration over time into changes in curvature of the swimming path.

Cited by 93 publications

References 53 publications

Seminal Plasma pH, Inorganic Phosphate, Total and Ionized Calcium Concentrations In The Assessment of Human Spermatozoa Function

Seminal Plasma pH, Inorganic Phosphate, Total and Ionized Calcium Concentrations In The Assessment of Human Spermatozoa Function

Cl− Channels and Transporters in Sperm Physiology

FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters

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