The rat phospholipase Cβ 4 gene is expressed at high abundance in cerebellar Purkinje cells

Genetic analysis of mammalian G-protein signalling

“…Cerebellar LTD is a form of synaptic plasticity believed to be a cellular substrate for some forms of motor learning and to involve the modulatory regulation of excitatory AMPA receptors at the PF-PC synapse (Ito, 1989;Linden, 1994) (Figure 1). Very similar phenotypes have been described for mice lacking the metabotropic glutamate type 1 receptor (mGluR1) (Aiba et al, 1994;Kano et al, 1997) which couples to Ga q/11 as well as for mice de®cient in the b4 isoform of phospholipase C which shows predominant expression in cerebellar Purkinje cells (Roustan et al, 1995) (H Jiang, M Kano, MIS, D Wu; unpublished observation). The mGluR1, Ga q and PLCb4 are colocalized in dendritic spines of PCs, suggesting that a de®ned signalling cascade in the postsynaptic membrane of PF-PC synapses is involved in the postnatal elimination of multiple CF innervation as well as in cerebellar LTD (Figure 1).…”

Altered PLCβ-1 expression in the gerbil hippocampal complex following spontaneous seizure

“…The major finding of the present study is that PLCβ-1 immunoreactivities were abundantly observed in the pyramidal cell layer of CA1-3 and in the dentate hilar neurons, as previously described patterns of expression of PLC β subtypes in the hippocampus (1,5,6,15,16). In addition, Fukaya et al (1) reported that the expression of PLCβ-1 is mainly observed in somato-dendritic components of principal neurons within the excitatory synapses of the hippocampus, which is similar to our results.…”

In vivo functions of heterotrimeric G-proteins: studies in Gα-deficient mice

“…A defect in the modulation of the PF-PC synapse in mice lacking Ga q is also suggested by the fact that long term depression (LTD) of the PF-PC synapse was de®cient in Ga q (7/ 7) mice (M Kano et al, unpublished). Very similar phenotypes have been described in mice lacking the metabotropic glutamate type 1 receptor (GluR1) (Aiba et al, 1994;Kano et al, 1997) which couples to Ga q/11 as well as in mice de®cient in the b4 isoform of phospholipase C which shows predominant expression in cerebellar Purkinje cells (Roustan et al, 1995). The mGluR1, Ga q and PLCb4 are colocalized in dendritic spines of PCs Kano et al, 1998;Tanaka et al, 2000), suggesting that a de®ned signaling cascade in the postsynaptic membrane of PF-PC synapses is involved in the postnatal elimination of multiple CF innervation as well as in cerebellar LTD.…”