The rapid, quantitative determination of neutral sugars (as aldononitrile acetates) and amino sugars (as O-methyloxime acetates) in glycoproteins by gas-liquid chromatography

Mawhinney, Thomas P.; Feather, Milton S.; Barbero, Giulio J.; Martinez, J. Ricardo

doi:10.1016/0003-2697(80)90048-2

Cited by 104 publications

(28 citation statements)

References 18 publications

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“…Samples are usually hydrolyzed with 6 M HCl although more details about hydrolysis procedures will be shown in Sections 9 and 10. Classical procedures of derivatization for carbohydrate analysis, such as alditol acetates, TMS ethers and aldononitrile acetates have been assayed [35,36].…”

Section: Aminosugarsmentioning

confidence: 99%

Derivatization of carbohydrates for GC and GC–MS analyses

Ruiz‐Matute

Hernández‐Hernández

Rodríguez-Sánchez

et al. 2011

Journal of Chromatography B

364

195

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Section: Aminosugarsmentioning

confidence: 99%

Derivatization of carbohydrates for GC and GC–MS analyses

Ruiz‐Matute

Hernández‐Hernández

Rodríguez-Sánchez

et al. 2011

Journal of Chromatography B

364

195

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“…The hydrolysate was reduced with NaBH 4 for 3 h at r.t. The excess NaBH 4 was decomposed with AcOH and removed by repeated evaporation to dryness with the addition of 10% (v/v) AcOH in MeOH [33]. The alditol acetates of the reduced sugars and authentic standards (rhamnose, arabinose, mannose, glucose, and galactose with inositol as the internal standard) were prepared with AC 2 O at 1008 for 1 h and subjected to GC analysis.…”

Section: Homentioning

confidence: 99%

Chemical Composition and Antioxidant Activities of Different Polysaccharides from the Roots of Angelica dahurica

et al. 2011

Chemistry & Biodiversity

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The total crude polysaccharides (CADPs), isolated from the roots of Angelica dahurica by H(2) O extraction, EtOH precipitation, and dialysis, and the four fractions ADP1, ADP2, ADP3, and ADP4, obtained by gel filtration of the CADPs, were analyzed to characterize their composition and evaluated for their antioxidant activity using different in vitro tests such as the malondialdehyde (MDA)-production, the ferrous ion (Fe(2+) )-chelating, and the HO(.) radical-scavenging assays. The predominant neutral monosaccharides in the four fractions were identified as arabinose, galactose, and glucose, while the composition and ratio of the monosaccharides were different between the fractions. The CADPs and its fractions were found to significantly inhibit lipid peroxidation, chelate Fe(2+) , and scavenge HO(.) radicals, indicating that these polysaccharides possessed antioxidant activity. Among the four fractions, ADP4 exhibited the strongest antioxidant activity, which was stronger than that of the control antioxidant vitamin E (Vit E). Taken together, the chemical composition of these polysaccharides might affect their antioxidant activity, and ADP4 could be explored as a source of potential novel natural antioxidants for food and pharmaceutical purposes.

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“…Calcium (method 984.01) and phosphorus (method 965.17) contents were determined according to the AOAC (1995). Lactose was analyzed by gas-liquid chromatography via trimethylsilyl-Omethyl-oxime derivatives (Mawhinney et al, 1980). The individual amino acid composition was measured using an amino acid analyzer (Beckman 6300, Beckman Coulter, Inc., Fullerton, California, US) after 24-h of 6 N-HCl hydrolysis at 110°C (AOAC, 2000).…”

Section: Chemical Analysismentioning

confidence: 99%

Effects of Chelated Copper and Zinc Supplementation on Growth Performance, Nutrient Digestibility, Blood Profiles, and Fecal Noxious Gas Emission in Weanling Pigs

Zhang¹,

Cho²,

Kim³

2013

Journal of Animal Science and Technology

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This study was conducted to evaluate the effects of chelated Cu and Zn on growth performance, nutrient digestibility, blood profiles, and fecal noxious gas emission in weanling pigs. A total of 90 weanling pigs with an initial body weight (BW) of 5.27 ± 0.04 kg were randomly allotted to two dietary treatments for 42 d. Pigs were then fed a control diet (CON) or a Zinpro diet (CON + 0.1% chelate copper and zinc). There were nine replicate-pens with five pigs in each pen. During d 0 to 14 and d 14 to 28, the ADFI decreased (p<0.05) and the G/F increased (p<0.05) in pigs fed the Zinpro diet compared with those that received the CON diet. During d 28 to 42, the ADFI increased (p<0.05) in pigs fed the Zinpro diet relative to those fed the CON diet. Additionally, the apparent total tract digestibility of DM, N, and energy increased (p<0.05) in the Zinpro group when compared to the CON group on d 14 and 28. The lymphocyte percentage was also greater (p<0.05) in the Zinpro group than in the CON group. Overall, dietary supplementation with 0.1% chelate copper and zinc improved the growth performance and nutrient digestibility in weanling pigs.

show abstract

The rapid, quantitative determination of neutral sugars (as aldononitrile acetates) and amino sugars (as O-methyloxime acetates) in glycoproteins by gas-liquid chromatography

Cited by 104 publications

References 18 publications

Derivatization of carbohydrates for GC and GC–MS analyses

Derivatization of carbohydrates for GC and GC–MS analyses

Chemical Composition and Antioxidant Activities of Different Polysaccharides from the Roots of Angelica dahurica

Effects of Chelated Copper and Zinc Supplementation on Growth Performance, Nutrient Digestibility, Blood Profiles, and Fecal Noxious Gas Emission in Weanling Pigs

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