The rapid diagnosis and effective inhibition of coronavirus using spike antibody attached gold nanoparticles

Pramanik, Avijit; Gao, Ye; Patibandla, Shamily; Mitra, Dipanwita; McCandless, Martin G.; Fassero, Lauren A; Gates, Kalein; Tandon, Ritesh; Ray, Paresh Chandra

doi:10.1039/d0na01007c

Cited by 97 publications

(151 citation statements)

References 41 publications

(290 reference statements)

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“…After the solution cooled naturally for another 10 min under constant stirring, the resulting colloidal gold solutions were filtered through a 0.45-μm membrane and stored in the dark at 4 °C until use. As the value of the extinction coefficient for the AuNPs with a diameter of 15 nm is 3.6 x 10 8 cm -1 M -1 [ 62 ], the concentration of the AuNPs with the approximate diameter of 15 nm was estimated to be 2.4 nM.…”

Section: Methodsmentioning

confidence: 99%

“…Due to those combined advantages, electrochemical and colorimetric biosensors and/or methods could be developed to determine molecular SARS-CoV-2 antigens, antibodies and/or their fragments. Indeed, in the past few months alone, several nanoscale integrated structures based on optical and electronic systems have been reportedly been able to detect the SARS-CoV-2 spike protein with high sensitivity [ [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] , [66] , [67] , [68] ].…”

Section: Introductionmentioning

confidence: 99%

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Colorimetric and electrochemical detection of SARS-CoV-2 spike antigen with a gold nanoparticle-based biosensor

Karakuş

Erdemir

Demirbilek

et al. 2021

Analytica Chimica Acta

110

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Since emerging in China in December 2019, COVID-19 has spread globally, wreaked havoc for public health and economies worldwide and, given the high infectivity and unexpectedly rapid spread of the virus responsible—that is, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—urged the World Health Organization to declare it a pandemic. In response, reducing the virus’s adverse effects requires developing methods of early diagnosis that are reliable, are inexpensive and offer rapid response. As demonstrated in this article, the colorimetric and electrochemical detection of SARS-CoV-2 spike antigens with gold nanoparticle-based biosensors may be one such method. In the presence of the SARS-CoV-2 antigen, gold nanoparticles aggregated rapidly and irreversibly due to antibody–antigen interaction and consequently changed in colour from red to purple, as easily observable with the naked eye or UV-Vis spectrometry by way of spectral redshifting with a detection limit of 48 ng/mL. Moreover, electrochemical detection was achieved by dropping developed probe solution onto the commercially available and disposable screen-printed gold electrode without requiring any electrode preparation and modification. The method identified 1 pg/mL of the SARS-CoV-2 spike antigen and showed a linear response to the SARS-CoV-2 spike antigen ranging from 1 pg/mL to 10 ng/mL. Both methods were highly specific to detecting the SARS-CoV-2 antigen but not other antigens, including influenza A (i.e. H1N1), MERS-CoV and Streptococcus pneumoniae , even at high concentrations.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Colorimetric and electrochemical detection of SARS-CoV-2 spike antigen with a gold nanoparticle-based biosensor

Karakuş

Erdemir

Demirbilek

et al. 2021

Analytica Chimica Acta

110

View full text Add to dashboard Cite

show abstract

“…Gold nanoparticles have been explored to detect mumps and zika virus (Hsu et al 2020;Long et al 2020). Gold nanozymes have been employed in rapid detection of SARS-CoV-19 (Pramanik et al 2021). Indeed, samples taken in positive patients contain nucleocapsid phosphoprotein oligonucleotides that combine with thiol-functionalized gold nanozymes, which allow detection in 10 min by calorimetry (Kamaraj 2020).…”

Section: Nanozymes For Detection Of Chemicals Viruses and Cellsmentioning

confidence: 99%

Nanozymes to fight the COVID-19 and future pandemics

et al. 2021

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In 2019, the pandemic induced by the rapid spread of the severe acute respiratory syndrome coronavirus-2 'SARS-CoV-2', also named coronavirus disease 'COVID-19', has rapidly caused serious health, economic, social, and political issues worldwide. This has been accentuated by asymptomatic carriers, fast viral mutations, incoherent lockdown policies, and limited methods of detection and treatment. Nucleic acid tests for COVID-19 are lengthy and complicated, and the rapid antibody tests produce false-negative diagnoses, particularly in the early stage of infection. Moreover, there are no specific and effective medicines available for treatment. Therefore, rapid diagnostics and therapeutics are urgently required. Here, we argue that nanozymesenzyme-mimicking nanoparticles-could be used for faster, more sensitive, and accurate detection and treatment of the COVID-19. Indeed, nanozymes have high surface areas allowing easier bioconjugation for detection, and nanozymes possess integrated drug-functionalization for treatment. Moreover, the size, composition, and surface chemistry of nanozymes can be rationally designed for improved theranostic applications. COVID-19 historyThe COVID-19 appeared in Wuhan, China, due to emergence of a novel strain of coronavirus (Sallard et al. 2021), which was provisionally named '2019-nCoV' by the World Health Organization (WHO). Later, the virus was renamed 'SARS-CoV-2' by the International Committee

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“…Pramanik et al [ 68 ] have employed antibody attached AuNPs for the naked eye detection of COVID-19 viral antigen or of pseudo SARS-CoV-2 using a very fast and simple colorimetric change. Based on 4-aminothiophenol and anti-spike antibody attached gold nanoparticle SERS, the antigen of COVID-19 or virus can be detected at very low concentrations within 5 min.…”

Section: Coronavirusesmentioning

confidence: 99%

Role of plasmonics in detection of deadliest viruses: a review

et al. 2021

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Viruses have threatened animal and human lives since a long time ago all over the world. Some of these tiny particles have caused disastrous pandemics that killed a large number of people with subsequent economic downturns. In addition, the quarantine situation itself encounters the challenges like the deficiency in the online educational system, psychiatric problems and poor international relations. Although viruses have a rather simple protein structure, they have structural heterogeneity with a high tendency to mutation that impedes their study. On top of the breadth of such worldwide worrying issues, there are profound scientific gaps, and several unanswered questions, like lack of vaccines or antivirals to combat these pathogens. Various detection techniques like the nucleic acid test, immunoassay, and microscopy have been developed; however, there is a tradeoff between their advantages and disadvantages like safety in sample collecting, invasiveness, sensitivity, response time, etc. One of the highly resolved techniques that can provide early-stage detection with fast experiment duration is plasmonics. This optical technique has the capability to detect viral proteins and genomes at the early stage via highly sensitive interaction between the biological target and the plasmonic chip. The efficiency of this technique could be proved using commercialized techniques like reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) techniques. In this study, we aim to review the role of plasmonic technique in the detection of 11 deadliest viruses besides 2 common genital viruses for the human being. This is a rapidly moving topic of research, and a review article that encompasses the current findings may be useful for guiding strategies to deal with the pandemics . By investigating the potential aspects of this technique, we hope that this study could open new avenues toward the application of point-of-care techniques for virus detection at early stage that may inhibit the progressively hygienic threats.

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The rapid diagnosis and effective inhibition of coronavirus using spike antibody attached gold nanoparticles

Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the coronavirus disease that began in 2019 (COVID-19), has been responsible for 1.4 million deaths worldwide as of 13 November 2020.

Cited by 97 publications

References 41 publications

Colorimetric and electrochemical detection of SARS-CoV-2 spike antigen with a gold nanoparticle-based biosensor

Colorimetric and electrochemical detection of SARS-CoV-2 spike antigen with a gold nanoparticle-based biosensor

Nanozymes to fight the COVID-19 and future pandemics

Role of plasmonics in detection of deadliest viruses: a review

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