The Rapid Carbapenemase Detection Method (rCDM) for Rapid and Accurate Detection of Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa

Jing, Xiaopeng; Min, Xiaochun; Zeng, Xing; Gong, Lin; Wu, Tingting; Sun, Ruiling; Chen, Liujun; Liu, Rong; Zeng, Jing

doi:10.3389/fcimb.2019.00371

Cited by 9 publications

(9 citation statements)

References 10 publications

(13 reference statements)

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“…Testing methods mentioned above are either take a long time (such as modified carbapenem inactivation method, mCIM, which requires 24 h to detection), considered expensive (MALDI-TOF MS), or require substances with limited shelf-life (Carba NP is stored in solution at 4-8°C for up to 3 days) (Jing et al 2019). If detection of carbapenemase strains can help prevent outbreaks of and treat A. baumannii infections, then rapid organism and carbapenemase detection method is recommended and should be further investigated.…”

Section: Roommentioning

confidence: 99%

Carbapenem resistance in Acinetobacter baumannii , and their importance in hospital‐acquired infections: a scientific review

2021

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Carbapenem is an important therapy for serious hospital-acquired infections and for the care of patients affected by multidrug-resistant organisms, specifically Acinetobacter baumannii; however, with the global increase of carbapenem-resistant A. baumannii, this pathogen has significantly threatened public health. Thus, there is a pressing need to better understand this pathogen in order to develop novel treatments and control strategies for dealing with A. baumannii. In this review, we discuss an overview of carbapenem, including its discovery, development, classification and biological characteristics, and its importance in hospital medicine especially in critical care units. We also describe the peculiarity of bacterial pathogen, A. baumannii, including its commonly reported virulence factors, environmental persistence and carbapenem resistance mechanisms. In closing, we discuss various control strategies for overcoming carbapenem resistance in hospitals and for limiting outbreaks. With the appearance of strains that resist carbapenem, the aim of this review is to highlight the importance of understanding this increasingly problematic healthcare-associated pathogen that creates significant concern in the field of nosocomial infections and overall public health. Carbapenem Development and history of carbapenemSafe and effective antibiotics play an important role in medical care and their development remains one of the

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Section: Roommentioning

confidence: 99%

Carbapenem resistance in Acinetobacter baumannii , and their importance in hospital‐acquired infections: a scientific review

2021

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“…Genotypic assays are the gold standard for detecting the carbapenemase genes. However, phenotypic detections are convenient and manageable, including biochemical tests, growth-based assays, immunochromatographic assays and the detection of carbapenem hydrolysis by MALDI-TOF MS ( Giske et al, 2011 ; Papagiannitsis et al, 2015 ; Silva et al, 2017 ; Caméléna et al, 2018 ; Jing et al, 2018 , 2019 ; CLSI, 2020 ; Petit et al, 2020 ). Among these phenotypic assays, only a few tests can determine the class of carbapenemase ( Giske et al, 2011 ; Silva et al, 2017 ; CLSI, 2020 ; Petit et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Detection and Characterization of Carbapenemases in Enterobacterales With a New Rapid and Simplified Carbapenemase Detection Method Called rsCDM

et al. 2022

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ObjectiveThis study aimed to develop a new rapid and simplified carbapenemase detection method (rsCDM) for detection and characterization of carbapenemase with 3-aminophenylboronic acid (APBA), ethylenediaminetetraacetic acid (EDTA), and cloxacillin (CLO) β-lactamase inhibitors.MethodsA panel of 182 carbapenem-resistant Enterobacterales (CRE) strains with blaKPC (88), blaNDM (60), blaIMP (10), blaVIM (3), blaOXA-181 (5), blaKPC, and blaNDM (7), porin changes in combination with an extended-spectrum β-lactamase (ESBL) (3) or AmpC hyper-production (6) and 43 carbapenem-susceptible Enterobacterales isolates were used to evaluate the performance of rsCDM and EDTA-carbapenem inactivation method (eCIM). Carbapenemase class was determined with specific inhibitors at 4, 6, and 18 h by rsCDM, and the difference between imipenem (IMI) and meropenem (MEM) disks was simultaneously compared.ResultsThe sensitivity of rsCDM using IMI was 97.1% at the three time points, with a specificity of 100%, independent of the culture duration. Similar to IPM, MEM disk also showed high sensitivity (97.1%) and specificity (100%) at 6 h. And the sensitivity of eCIM was 95.4% and the specificity was 100%. Based on a decision algorithm, the characterization number of IMI and MEM in KPC-producing isolates was 88 vs. 87, metallo-β-lactamases (MBLs) was 73 vs. 72, KPC and NDM carbapenemase was 7 vs. 7 at 4 h, respectively. After 6 h, the category number changed insignificantly except for isolates with combined AmpC overproduction and porin changes, showing an increase in IMI (6) and MEM (2), and there was no difference in the results between 6 and 18 h for the two tablets. OXA-181-producing strains can’t be distinguished by rsCDM. For eCIM, the characterization number in KPC-, OXA- 181-, and MBLs-producing strains was 88, 5, and 72, but it failed to detect multi-enzyme-producing isolates (KPC and NDM).ConclusionrsCDM accurately discriminated carbapenemase within 4 h and could differentiate multi-enzyme (KPC and NDM) and AmpC in conjunction with porin changes strains. Hence, rsCDM represents a rapid, simple, easy readout, and accurate tool that can be used without any specialized equipment.

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“…Compared with other carbapenemase phenotype detection methods described these years, [21][22][23][24] the combined mrCIM and reCIM have several distinct advantages. Firstly, the detection time is less than 4 hrs, 1 day shorter than the mCIM and eCIM or other reported methods.…”

Section: Discussionmentioning

confidence: 99%

<p>Evaluation of Modified Rapid Carbapenem Inactivation Method (mrCIM) Combined with Rapid EDTA-Modified Carbapenem Inactivation Method (reCIM) to Detect Carbapenemase and Distinguish Metallo-Carbapenemase in <em>Enterobacteriaceae</em> Within Four Hours</p>

Wei¹,

Jin²,

Wang³

et al. 2020

IDR

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Purpose: To develop a rapid EDTA-modified carbapenem inactivation method (reCIM) combined with modified rapid carbapenem inactivation method (mrCIM) to detect carbapenemase and distinguish metallo-β-lactamases from carbapenemases in Enterobacteriaceae in 4 hrs. Materials and Methods: The sensitivities and specificities of mrCIM and reCIM were retrospectively evaluated in 247 carbapenem-resistant Enterobacteriaceae of which 107 were carbapenemase producers confirmed by PCR and sequencing. In addition, mrCIM and reCIM were prospectively evaluated with 47 carbapenem-resistant enterobacterial isolates. Results: The sensitivity and specificity of mrCIM were 96.3% and 97.1% at 2.5 hrs post incubation, and the specificity increased to 98.6% at 3 hrs. The combined mrCIM and reCIM showed a sensitivity of 95.4% and a specificity of 100% at 2.5 hrs post incubation in identifying metallo-β-lactamases, and the sensitivity increased to 97.0% at 3 hrs. These performance characteristics are comparable to mCIM and eCIM; however, compared with mCIM and reCIM tests which need at least 24 hrs to detect results, the mrCIM and reCIM required less than 4 hrs of total work time. Conclusion:The combined mrCIM and reCIM can be used to accurately and quickly detect carbapenemase and metallo-β-lactamases in Enterobacteriaceae in 4 hrs and are suitable for routine use in most clinical microbiology laboratories.

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The Rapid Carbapenemase Detection Method (rCDM) for Rapid and Accurate Detection of Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa

Cited by 9 publications

References 10 publications

Carbapenem resistance in Acinetobacter baumannii , and their importance in hospital‐acquired infections: a scientific review

Carbapenem resistance in Acinetobacter baumannii , and their importance in hospital‐acquired infections: a scientific review

Detection and Characterization of Carbapenemases in Enterobacterales With a New Rapid and Simplified Carbapenemase Detection Method Called rsCDM

<p>Evaluation of Modified Rapid Carbapenem Inactivation Method (mrCIM) Combined with Rapid EDTA-Modified Carbapenem Inactivation Method (reCIM) to Detect Carbapenemase and Distinguish Metallo-Carbapenemase in <em>Enterobacteriaceae</em> Within Four Hours</p>

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