The rapid and specific real-time detection of Legionella pneumophila in water samples using Optical Waveguide Lightmode Spectroscopy

Cooper, Ian; Meikle, Steve; Standen, Guy; Hanlon, G. W.; Santin, Matteo

doi:10.1016/j.mimet.2009.04.004

Cited by 20 publications

(17 citation statements)

References 16 publications

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“…The isolates that grow on BCYE but fail to grow on BCYE without L-cysteine, can be presumptively identified as Legionella spp. [24,47]. Closer identification can be carried out using latex agglutination, direct immunofluoresence or molecular techniques.…”

Section: Discussionmentioning

confidence: 99%

“…The correct identification of species is also important for epidemiological studies and identification of sources of infection [65]. However, the major disadvantage of PCR it is the inability to evaluate the viability, in other words, by the PCR technique it is not possible to distinguish between viable and nonviable microorganisms, detecting only their presence or absence, while only viable bacteria are able to cause infections in human and represent an interest for public health [20,23,47]. The qPCR is an important complement, but not a substitute, for the standard culture-based methods [66].…”

Section: Discussionmentioning

confidence: 99%

“…A rapid, sensitive, and specific method for the detection of Legionella is clearly important to identify infected individuals and to expedite cleanup of contaminated water systems (reduction in disinfecting treatment cost) in order to prevent additional cases of infection [25,47].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of <i>Legionella</i> spp. in Natural and Man-made Water Systems Using Standard Guidelines

Borges¹,

Simes²,

Martínez‐Murcia³

et al. 2012

MICROBIOLOGY

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Infections caused by Legionella spp. are considered at the present time, an emerging public health problem and are linked to high rates of mortality and morbidity, if not properly treated. In this study were analyzed 54 samples of water from 8 counties at Northern Portugal, with the aim of obtaining a collection of strains of the genus Legionella and to characterize them genetically and phenotypically. Another objective of this study was to evaluate the effectiveness of the technique of cultivation, a standard method according to International Organization for Standardization ISO 11731:1998, for detection and enumeration of species of Legionella. For laboratory processing, after the filtration of samples (1 L), the filtrate was resuspended in sterile distilled water (5 ml). Heat treatment for selective inhibition of non-Legionella bacteria was performed. Subsequently, 100 μl of the suspension was spread in GVPC selective agar medium, and incubated (7 to 10 days) at 37 ℃. Colonies that were morphologically characteristic of the genus were sub-cultured onto BCYE agar and blood agar for verification. According to the procedure recommended by the standard method, only the colonies which grew in BCYE agar and not on blood agar were considered as suspected Legionella strains. The identification of these initially selected colonies was performed by sequencing the 16S rRNA gene, which revealed that none of the isolates were identified as belonging to the genus Legionella. However, through the ISO 11731:1998 they were interpreted as positive, corresponding therefore to "false-positive" results. The methods used in this study allowed the isolation of a number of isolates (40), which form an independent group of all genus of the family Chitinophagaceae outlined so far, and that by their phylogenetic distance might be a genus not yet described and therefore a new species. The results obtained, highlighted the importance of using culture and genetic methods in parallel for the proper identification of microorganisms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Detection of <i>Legionella</i> spp. in Natural and Man-made Water Systems Using Standard Guidelines

Borges¹,

Simes²,

Martínez‐Murcia³

et al. 2012

MICROBIOLOGY

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show abstract

“…The detection of L. pneumophila in water samples using standard microbiological culture techniques is a delayed process because the bacterium is slow-growing and nutritionally fastidious, to the point that other species may compete with Legionella even when antibiotic are supplemented (Cooper et al, 2009). …”

Section: Bruno Et Al Described a Fluorescence Resonance Energy Transmentioning

confidence: 99%

“…The assay is presented as a rapid (25 min.) and sensitive (1.3×10 4 CFU mL −1 ) detection method for L. pneumophila contamination in water samples (Cooper et al, 2009).…”

Section: Bruno Et Al Described a Fluorescence Resonance Energy Transmentioning

confidence: 99%

Emerging (Bio)Sensing Technology for Assessing and Monitoring Freshwater Contamination - Methods and Applications

Queirós

Noronha²,

Sales³

2012

Ecological Water Quality - Water Treatment and Reuse

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Optical Waveguide Biosensors

Duval¹,

Lechuga²

2015

Photonics

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The rapid and specific real-time detection of Legionella pneumophila in water samples using Optical Waveguide Lightmode Spectroscopy

Cited by 20 publications

References 16 publications

Detection of <i>Legionella</i> spp. in Natural and Man-made Water Systems Using Standard Guidelines

Detection of <i>Legionella</i> spp. in Natural and Man-made Water Systems Using Standard Guidelines

Emerging (Bio)Sensing Technology for Assessing and Monitoring Freshwater Contamination - Methods and Applications

Optical Waveguide Biosensors

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