The RanBP2/RanGAP1*SUMO1/Ubc9 complex

Flotho, Annette; Werner, Andreas

doi:10.4161/nucl.21980

Cited by 15 publications

(5 citation statements)

References 31 publications

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“…52,53 One of the best characterized SUMO pathways is the SUMO E3 ligase RanBP2 (Nup358) involving the Ran GTPase (E1) activating protein (RanGAP1). 54,55 The SUMO E3 ligase RanBP2(Nup358) complex impacts upon nucleoplasmic transport, microtubule binding and organization, as well as spindle assembly and disassembly 56,57 functions, similar to those proposed for the ALMS1 gene.…”

Section: Introductionmentioning

confidence: 79%

Coding and noncoding expression patterns associated with rare obesity-related disorders: Prader–Willi and Alström syndromes

Butler¹,

Wang²,

Marshall³

et al. 2015

AGG

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Obesity is accompanied by hyperphagia in several classical genetic obesity-related syndromes that are rare, including Prader–Willi syndrome (PWS) and Alström syndrome (ALMS). We compared coding and noncoding gene expression in adult males with PWS, ALMS, and nonsyndromic obesity relative to nonobese males using readily available lymphoblastoid cells to identify disease-specific molecular patterns and disturbed mechanisms in obesity. We found 231 genes upregulated in ALMS compared with nonobese males, but no genes were found to be upregulated in obese or PWS males and 124 genes were downregulated in ALMS. The metallothionein gene (MT1X) was significantly downregulated in ALMS, in common with obese males. Only the complex SNRPN locus was disturbed (downregulated) in PWS along with several downregulated small nucleolar RNAs (snoRNAs) in the 15q11-q13 region (SNORD116, SNORD109B, SNORD109A, SNORD107). Eleven upregulated and ten downregulated snoRNAs targeting multiple genes impacting rRNA processing, developmental pathways, and associated diseases were found in ALMS. Fifty-two miRNAs associated with multiple, overlapping gene expression disturbances were upregulated in ALMS, and four were shared with obese males but not PWS males. For example, seven passenger strand microRNAs (miRNAs) (miR-93*, miR-373*, miR-29b-2*, miR-30c-1*, miR27a*, miR27b*, and miR-149*) were disturbed in association with six separate downregulated target genes (CD68, FAM102A, MXI1, MYO1D, TP53INP1, and ZRANB1). Cell cycle (eg, PPP3CA), transcription (eg, POLE2), and development may be impacted by upregulated genes in ALMS, while downregulated genes were found to be involved with metabolic processes (eg, FABP3), immune responses (eg, IL32), and cell signaling (eg, IL1B). The high number of gene and noncoding RNA disturbances in ALMS contrast with observations in PWS and males with nonsyndromic obesity and may reflect the progressing multiorgan pathology of the ALMS disease process.

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Section: Introductionmentioning

confidence: 79%

Coding and noncoding expression patterns associated with rare obesity-related disorders: Prader–Willi and Alström syndromes

Butler¹,

Wang²,

Marshall³

et al. 2015

AGG

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“…RanGAP1 and RanBP2 contribute to the nuclear import of proteins [ 23 , 24 ]. Recently, the RanBP2/RanGAP1 * SUMO1/UBC9 complex was reported to be a multisubunit SUMO E3 ligase in the NPC that regulates the nuclear import of SUMOylated target proteins [ 25 , 26 ]. Accordingly, SUMO1 promotes the nuclear translocation of target proteins, such as SAE2, ZIC3, and ErbB4 [ 27 – 29 ].…”

Section: Discussionmentioning

confidence: 99%

Small ubiquitin-related modifier 1 is involved in hepatocellular carcinoma progression via mediating p65 nuclear translocation

Tao

Zhang

et al. 2016

Oncotarget

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Small ubiquitin-related modifier (SUMO) proteins participate in a post-translational modification called SUMOylation and regulate a variety of intracellular processes, such as targeting proteins for nuclear import. The nuclear transport of p65 results in the activation of NF-κB, and p65 contains several SUMO interacting motifs (SIMs). However, the relationship between p65 and SUMO1 in hepatocellular carcinoma (HCC) remains unclear. In this study, we demonstrated the potential roles of SUMO1 in HCC via the regulation of p65 subcellular localization. We found that either SUMO1- or p65-positive immunoreactivity was remarkably increased in the nuclei of tumor tissues in HCC patients compared with non-tumor tissues, and further analysis suggested a correlation between SUMO1- and nuclear p65-positive immunoreactivities (R = 0.851, P = 0.002). We also verified the interaction between p65 and SUMO1 in HCC by co-immunoprecipitation. TNF-α and hypoxia increased SUMO1 protein levels and enhanced SUMO1-modified p65 SUMOylation. Moreover, the knockdown of SUMO1 decreased p65 nuclear translocation and inhibited NF-κB transcriptional activity. Further the results of this study revealed that the knockdown of SUMO1 suppressed the proliferation and migration of hepatoma cells. These results suggest that SUMO1 contributes to HCC progression by promoting p65 nuclear translocation and regulating NF-κB activity.

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“… The main interface of IBS. ( A ) The standalone software showing the domain organization of E3 SUMO-protein ligase RanBP2 ( Flotho and Werner, 2012 ). ( B ) The online service presenting the organization of bromodomain proteins and translocations in cancer ( Muller et al.…”

Section: The Protein Modementioning

confidence: 99%

IBS: an illustrator for the presentation and visualization of biological sequences

et al. 2015

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Summary: Biological sequence diagrams are fundamental for visualizing various functional elements in protein or nucleotide sequences that enable a summarization and presentation of existing information as well as means of intuitive new discoveries. Here, we present a software package called illustrator of biological sequences (IBS) that can be used for representing the organization of either protein or nucleotide sequences in a convenient, efficient and precise manner. Multiple options are provided in IBS, and biological sequences can be manipulated, recolored or rescaled in a user-defined mode. Also, the final representational artwork can be directly exported into a publication-quality figure.Availability and implementation: The standalone package of IBS was implemented in JAVA, while the online service was implemented in HTML5 and JavaScript. Both the standalone package and online service are freely available at http://ibs.biocuckoo.org.Contact: renjian.sysu@gmail.com or xueyu@hust.edu.cnSupplementary information: Supplementary data are available at Bioinformatics online.

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The RanBP2/RanGAP1*SUMO1/Ubc9 complex

Cited by 15 publications

References 31 publications

Coding and noncoding expression patterns associated with rare obesity-related disorders: Prader–Willi and Alström syndromes

Coding and noncoding expression patterns associated with rare obesity-related disorders: Prader–Willi and Alström syndromes

Small ubiquitin-related modifier 1 is involved in hepatocellular carcinoma progression via mediating p65 nuclear translocation

IBS: an illustrator for the presentation and visualization of biological sequences

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The RanBP2/RanGAP1*SUMO1/Ubc9 complex

Cited by 15 publications

References 31 publications

Coding and noncoding expression patterns associated with rare obesity-related disorders: Prader&ndash;Willi and Alstr&ouml;m syndromes

Coding and noncoding expression patterns associated with rare obesity-related disorders: Prader&ndash;Willi and Alstr&ouml;m syndromes

Small ubiquitin-related modifier 1 is involved in hepatocellular carcinoma progression via mediating p65 nuclear translocation

IBS: an illustrator for the presentation and visualization of biological sequences

Contact Info

Product

Resources

About

Coding and noncoding expression patterns associated with rare obesity-related disorders: Prader–Willi and Alström syndromes

Coding and noncoding expression patterns associated with rare obesity-related disorders: Prader–Willi and Alström syndromes