The Radiosensitizing Effect of Nanodiamonds (NDs) on HeLa Cells Under X‐Ray Irradiation

Chen, Xin; Wang, Hong; Wang, Hao; Fu, Yang; Liu, Jie; Liu, Runze

doi:10.1002/pssa.201700715

Cited by 6 publications

(4 citation statements)

References 28 publications

(29 reference statements)

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“…Even though nuclear DNA is considered the main target for ionizing radiation [51,53], our findings show that NDs localized in the cytoplasm and did not penetrate the cell nucleus. Therefore, although the possibility that the interaction between nanoparticles and radiation might produce secondary electrons/radicals occasionally reaching the nucleus and damaging DNA may not be completely ruled out, it is unlikely that this damage can account for the increase in radiation-induced cell death by nanoparticles.…”

Section: Discussionmentioning

confidence: 61%

Nanodiamond Effects on Cancer Cell Radiosensitivity: The Interplay between Their Chemical/Physical Characteristics and the Irradiation Energy

Varzi,

Fratini,

Falconieri

et al. 2023

IJMS

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Nanoparticles are being increasingly studied to enhance radiation effects. Among them, nanodiamonds (NDs) are taken into great consideration due to their low toxicity, inertness, chemical stability, and the possibility of surface functionalization. The objective of this study is to explore the influence of the chemical/physical properties of NDs on cellular radiosensitivity to combined treatments with radiation beams of different energies. DAOY, a human radioresistant medulloblastoma cell line was treated with NDs—differing for surface modifications [hydrogenated (H-NDs) and oxidized (OX-NDs)], size, and concentration—and analysed for (i) ND internalization and intracellular localization, (ii) clonogenic survival after combined treatment with different radiation beam energies and (iii) DNA damage and apoptosis, to explore the nature of ND–radiation biological interactions. Results show that chemical/physical characteristics of NDs are crucial in determining cell toxicity, with hydrogenated NDs (H-NDs) decreasing either cellular viability when administered alone, or cell survival when combined with radiation, depending on ND size and concentration, while OX-NDs do not. Also, irradiation at high energy (γ-rays at 1.25 MeV), in combination with H-NDs, is more efficient in eliciting radiosensitisation when compared to irradiation at lower energy (X-rays at 250 kVp). Finally, the molecular mechanisms of ND radiosensitisation was addressed, demonstrating that cell killing is mediated by the induction of Caspase-3-dependent apoptosis that is independent to DNA damage. Identifying the optimal combination of ND characteristics and radiation energy has the potential to offer a promising therapeutic strategy for tackling radioresistant cancers using H-NDs in conjunction with high-energy radiation.

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Section: Discussionmentioning

confidence: 61%

Nanodiamond Effects on Cancer Cell Radiosensitivity: The Interplay between Their Chemical/Physical Characteristics and the Irradiation Energy

Varzi,

Fratini,

Falconieri

et al. 2023

IJMS

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“…Particularly in biomedicine, OLCs play significant roles in biocatalysis [2] , bioelectronics [3] , and biosensors [4] . In addition, OLCs can be absorbed by cells as a radiation sensitizer, interacting with intracellular components and influencing cell apoptosis [5] . Currently, more research into the biochemical properties of OLCs is necessary, including their cellular uptake.…”

Section: Introductionmentioning

confidence: 99%

Detecting intracellular onion-like carbon nanostructures based on cell biolens’ properties by holographic flow cytometry

Li,

Liu,

Feng

et al. 2024

Sixth Conference on Frontiers in Optical Imaging and Technology: Applications of Imaging Technologies

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In recent years, onion-like carbons (OLCs), as a new type of carbon nanostructure, have emerged as significant in biomedical fields. OLCs are capable of being internalized by cells, interacting with various organelles, and thereby influencing cellular physiological processes. Consequently, there is considerable interest in the rapid, non-invasive detection and statistical analysis of intracellular OLCs. Holographic flow cytometry provides a high-throughput, label-free imaging approach, introducing a new approach for detecting intracellular OLCs. Indeed, cells can act as biological lenses, and the presence of intracellular OLCs alters their refractive index distribution, affecting their optical focusing and lensing features. In this study, we merged the 3D refractive index distribution of cells, obtained through in-flow tomographic experiments, with appropriate numerical simulations. This combination demonstrates that intracellular OLCs can be effectively detected by analyzing 2D quantitative phase maps, without the need for additional manual labeling. The experiments were conducted on colon cancer cells, both with and without intracellular OLCs. The results indicate that the biolens properties of cells can serve as a valuable biomarker for detecting intracellular OLCs. This promotes the research on OLCs-related physiological processes using holographic flow cytometry, enabling high-throughput, non-invasive screening of statistically significant number of cells.

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“…Despite this progress, it is still a great challenge to further enhance the anticorrosion performance of the WEP coatings. Nanodiamonds (NDs) possess excellent mechanical properties, electrical insulation, and physical barrier characteristics, 20,21 which can be potential nanofillers for WEP coatings to improve the anticorrosion performance. However, so far, relatively little attention has been paid to the NDs used as nanofillers for WEP coatings.…”

Section: Introductionmentioning

confidence: 99%

Monodispersed Nanodiamonds for Enhanced Anticorrosion of Waterborne Epoxy Coatings

Pan,

Zhang,

Zhang

et al. 2023

Ind. Eng. Chem. Res.

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Nanodiamonds (NDs) prepared by the explosion method possess very large surface energies, resulting in serious agglomerates, limiting their further application. Herein, we employed the strong shearing action of hydroxyl ion (OH − ) to induce the hydrolysis of esters between the ND primary particles, generating the carboxyl and hydroxyl groups, breaking hard agglomeration of NDs, and successfully constructed electrical double layers on the surface of NDs via deprotonation of surface oxygen-containing groups induced by the strong shearing action of hydroxyl ion (OH − ), leading to a significant increase of zeta potential absolute value (−54.2 mV), achieving the long-term stable (200 days) monodispersion of NDs (m-NDs). The asobtained m-NDs, as efficient nanofillers, were added into the waterborne epoxy (WEP) to produce a composite coating of m-NDs/WEP, displaying a considerable improvement in anticorrosion performance. The results of electrochemical impedance spectroscopy show that the |Z| f = 0.01 Hz of m-NDs/WEP is nearly 3 orders of magnitude higher than that of pure WEP after 168 h of immersion. The excellent anticorrosion performance of m-NDs/WEP can be ascribed to the excellent compatibility, electrical insulation property, and physical barrier behavior of m-NDs, inhibiting the infiltration of corrosive medium. The molecular dynamics simulation reveals that m-NDs have good barrier properties against a corrosive medium.

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The Radiosensitizing Effect of Nanodiamonds (NDs) on HeLa Cells Under X‐Ray Irradiation

Cited by 6 publications

References 28 publications

Nanodiamond Effects on Cancer Cell Radiosensitivity: The Interplay between Their Chemical/Physical Characteristics and the Irradiation Energy

Nanodiamond Effects on Cancer Cell Radiosensitivity: The Interplay between Their Chemical/Physical Characteristics and the Irradiation Energy

Detecting intracellular onion-like carbon nanostructures based on cell biolens’ properties by holographic flow cytometry

Monodispersed Nanodiamonds for Enhanced Anticorrosion of Waterborne Epoxy Coatings

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