“…A poor medium (PM1) was prepared with the same phosphate buffer and contained (in g/L) (NH 4 ) 2 SO 4 (1.5), sodium citrate (5), MgSO 4 ·7H 2 O (0.25), and 50 ml of trace element solution. The trace element solution contained (in mg/L) EDTA (500), MnCl 2 ·4H 2 O (10), CoCl 2 ·6H 2 O (4), Na 2 MoO 4 ·2H 2 O (3), CuSO 4 (1), ZnCl 2 (2), LiCl (0.5), SnCl 2 ·2H 2 O (0.5), H 3 BO 3 (1), KBr (2), KI (2), BaCl 2 (0.5), FeSO 4 (40), and CaCl 2 (5). Both PM1 and RM were supplemented with either 5 g/L of glycerol and designated PM1GLY and RMGLY, or with 5 g/L of separately heat-sterilized glucose and designated PM1GLU and RMGLU, respectively.…”