The “Race for the Surface” experimentally studied: In vitro assessment of Staphylococcus spp. adhesion and preosteoblastic cells integration to doped Ti-6Al-4V alloys

Martínez-Pérez, Marta; Conde, A.; Arenas, M.A.; Mahíllo‐Fernández, Ignacio; Damborenea, J. de; Pérez‐Tanoira, Ramón; Pérez‐Jorge, Concepción; Esteban, Jaime

doi:10.1016/j.colsurfb.2018.10.076

Cited by 9 publications

(12 citation statements)

References 64 publications

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“…The moment an implant abutment is inserted into the oral cavity bacteria and eukaryotic cells conquer for the settlement on the surface and for the integration of the implant 6 . This so‐called “race for the surface” describes an evolutionary fight for the alloplastic material surface 6,8,9 . If bacteria can successfully adhere to the surface and produce a biofilm of a critical size the successful integration of the surface by eukaryotic cells like gingival fibroblast or endothelia cells into the surrounding tissue cannot be achieved anymore 6,9 .…”

Section: Introductionmentioning

confidence: 99%

“…This so‐called “race for the surface” describes an evolutionary fight for the alloplastic material surface 6,8,9 . If bacteria can successfully adhere to the surface and produce a biofilm of a critical size the successful integration of the surface by eukaryotic cells like gingival fibroblast or endothelia cells into the surrounding tissue cannot be achieved anymore 6,9 . If the surface is covered by a dense layer of eukaryotic cells and successfully integrated into the surrounding tissue instead, bacteria cannot build a biofilm on the surface and cannot get to the underlying bone and cause infections 6,9 .…”

Section: Introductionmentioning

confidence: 99%

“…If bacteria can successfully adhere to the surface and produce a biofilm of a critical size the successful integration of the surface by eukaryotic cells like gingival fibroblast or endothelia cells into the surrounding tissue cannot be achieved anymore 6,9 . If the surface is covered by a dense layer of eukaryotic cells and successfully integrated into the surrounding tissue instead, bacteria cannot build a biofilm on the surface and cannot get to the underlying bone and cause infections 6,9 . This race for the surface takes place for every alloplastic material inserted into the human body, but especially the oral cavity with its enormous amount of microorganisms is a perfect example and measures have to be taken to change the outcome and to reduce possible post‐operative complications 9 …”

Section: Introductionmentioning

confidence: 99%

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Enhancing the soft‐tissue integration of dental implant abutments—in vitro study to reveal an optimized microgroove surface design to maximize spreading and alignment of human gingival fibroblasts

et al. 2021

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Within this work, we demonstrate the influences of different microgrooved surface topographies on the alignment and spreading of human gingival fibroblast (HGF) cells and present the optimal parameters for an improved soft‐tissue integration design for dental implant abutments for the first time. Microgrooves with lateral widths from 2.5 to 75 μm were fabricated by UV‐lithography and wet etching on bulk Ti6Al4V ELI material. The microstructured surfaces were compared to polished and ground surfaces as current state of the art. The resulting microtopographies were analyzed using vertical scanning interferometry and scanning electron microscopy. Samples loaded with HGF cells were incubated for 8 and 72 hr and cell orientation, spreading, resulting area, and relative gene expression were analyzed. The effect of contact guidance occurred on all microstructured surfaces yet there is a clear preferable range for the lateral widths of the microgrooves between approx. 11.5 and 13.9 μm and depths between 1.6 and 2.4 μm for an abutment surface design, where cell orientation and spreading maximizes. For structures larger than 30 μm, cell orientation, spreading and even gene expression of intercellular adhesion molecule‐1 and yes‐associated protein decrease.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhancing the soft‐tissue integration of dental implant abutments—in vitro study to reveal an optimized microgroove surface design to maximize spreading and alignment of human gingival fibroblasts

et al. 2021

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“…One of the most important causes of early and late orthopedic implant failure is inflammation of surrounding bone and soft tissues due to microbial contamination [ [1] , [2] , [3] , [4] ]. Since prevention of bacterial adhesion without the use of medication is the best way to reduce infection [ 5 ], it is desirable to develop novel antibacterial alloys or coatings on orthopedic implants to minimize colonization formation.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo antibacterial performance of Zr & O PIII magnesium alloys with high concentration of oxygen vacancies

Liang

Zeng

Shi

et al. 2021

Bioactive Materials

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The effects of dual Zr and O plasma immersion ion implantation (Zr & O PIII) on antibacterial properties of ZK60 Mg alloys are systematically investigated. The results show that a hydrophobic, smooth, and ZrO 2 -containing graded film is formed. Electrochemical assessment shows that the corrosion rate of the plasma-treated Mg alloy decreases and the decreased degradation rate is attributed to the protection rendered by the surface oxide. In vitro and in vivo antibacterial tests reveal Zr & O PIII ZK60 presents higher antibacterial rate compared to Zr PIII ZK60 and untreated control. The hydrophobic and smooth surface suppresses bacterial adhesion. High concentration of oxygen vacancies in the surface films are determined by X-ray photoelectron spectroscopy (XPS), UV–vis diffuse reflectance spectra (UV–vis DRS) and electron paramagnetic resonance (EPR) and involved in the production of reactive oxygen species (ROS). The higher level of ROS expression inhibits biofilm formation by down-regulating the expression of icaADBC genes but up-regulating the expression of icaR gene. In addition, Zr & O PIII improves cell viability and initial cell adhesion confirming good cytocompatibility. Dual Zr & O PIII is a simple and practical means to expedite clinical acceptance of biodegradable magnesium alloys.

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“…Chitosan/heparin PEMs were adsorbed onto nanotubes because heparin and chitosan have antimicrobial properties and heparin has been used to bind gentamicin to surfaces. ,− ,,,, Chitosan/heparin PEMs can present bone morphogenetic protein 2 (BMP-2) on nanotubes and increase the osteogenic responses of bone marrow cells for up to 4 weeks in vitro . While previous studies have tested the antimicrobial properties of surfaces and cellular attachment, very few have cocultured mammalian cells with bacteria to determine whether surfaces can simultaneously inhibit bacteria and promote mammalian cell attachment. ,− ,,,,− Infection of a prosthetic implant is described as a “race to the surface,” in which the chance of infection is determined by which colonizes the surfaces first, bacteria or osteogenic cells. If osteogenic cells colonize the surface first, then no infection happens because the bacteria die off; however, if bacteria colonize the surface first, then infection ensues .…”

Section: Introductionmentioning

confidence: 99%

Gentamicin-Releasing Titania Nanotube Surfaces Inhibit Bacteria and Support Adipose-Derived Stem Cell Growth in Cocultures

Wigmosta

Popat

Kipper

2021

ACS Appl. Bio Mater.

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Infection is the second leading cause of failure of orthopedic implants following incomplete osseointegration. Materials that increase the antimicrobial properties of surfaces while maintaining the ability for bone cells to attach and proliferate could reduce the failure rates of orthopedic implants. In this study, titania nanotubes (Nts) were modified with chitosan/heparin polyelectrolyte multilayers (PEMs) for gentamicin delivery. The antimicrobial activity of the surfaces was tested by coculturing bacteria with mammalian cells. Over 60% of gentamicin remained on the surface after an initial burst release on the first day. Antimicrobial activity of these surfaces was determined by exposure to Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) for up to 24 h. Gentamicin surfaces had less live E. coli and S. aureus by 6 h and less E. coli by 24 h compared to Nt surfaces. S. aureus and human adiposederived stem cells (hADSCs) were cocultured on surfaces for up to 7 days to characterize the so-called "race to the surface" between bacteria and mammalian cells, which is hypothesized to ultimately determine the outcome of orthopedic implants. By day 7, there was no significant difference in bacteria between surfaces with gentamicin adsorbed on the surface and surfaces with gentamicin in solution. However, gentamicin delivered in solution is toxic to hADSCs. Alternatively, gentamicin presented from PEMs enhances the antimicrobial properties of the surfaces without inhibiting hADSC attachment and cell growth. Delivering gentamicin from the surfaces is therefore superior to delivering gentamicin in solution and represents a strategy that could improve the antimicrobial activity of orthopedic implants and reduce risk of failure due to infection, without reducing mammalian cell attachment.

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The “Race for the Surface” experimentally studied: In vitro assessment of Staphylococcus spp. adhesion and preosteoblastic cells integration to doped Ti-6Al-4V alloys

Cited by 9 publications

References 64 publications

Enhancing the soft‐tissue integration of dental implant abutments—in vitro study to reveal an optimized microgroove surface design to maximize spreading and alignment of human gingival fibroblasts

Enhancing the soft‐tissue integration of dental implant abutments—in vitro study to reveal an optimized microgroove surface design to maximize spreading and alignment of human gingival fibroblasts

In vitro and in vivo antibacterial performance of Zr & O PIII magnesium alloys with high concentration of oxygen vacancies

Gentamicin-Releasing Titania Nanotube Surfaces Inhibit Bacteria and Support Adipose-Derived Stem Cell Growth in Cocultures

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