Abstract:Quorum sensing (QS) are intercellular communication mechanisms to coordinate bacterial gene expression in response to signaling molecules. In Bacillus thuringiensis the QS system NprR-NprRB (receptor protein-signaling peptide) regulates the expression of genes related to nutrient scavenging during necrotrophism and also modulates sporulation onset. However, the relevance of QS in free-living stages of B. thuringiensis is less known. In this work, we depict the contribution of this QS system to spreading in col… Show more
“…E. coli -GFP carries the pHT315 plasmid, which contains the bla gene for ampicillin resistance 45 and was modified in previous work by inserting a transcriptional fusion consisting of the P spac promoter and the gfp gene, resulting in constitutive GFP fluorescence. 46 The strains Bacillus sp. NME 255 (NME255), Burkholderia sp.…”
A simplified microfluidic high-throughput methodology coupled with Fluorescence Activated Cell Sorting (FACS) is used to screen, sort and recover antibiotic-producing bacterial strains.
“…E. coli -GFP carries the pHT315 plasmid, which contains the bla gene for ampicillin resistance 45 and was modified in previous work by inserting a transcriptional fusion consisting of the P spac promoter and the gfp gene, resulting in constitutive GFP fluorescence. 46 The strains Bacillus sp. NME 255 (NME255), Burkholderia sp.…”
A simplified microfluidic high-throughput methodology coupled with Fluorescence Activated Cell Sorting (FACS) is used to screen, sort and recover antibiotic-producing bacterial strains.
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