The Quinovic Acid Glycosides Purified Fraction from Uncaria tomentosa Protects against Hemorrhagic Cystitis Induced by Cyclophosphamide in Mice

Abstract

Uncaria tomentosa is widely used in folk medicine for the treatment of numerous diseases, such as urinary tract disease. Hemorrhagic cystitis (HE) is an inflammatory condition of the bladder associated with the use of anticancer drugs such as cyclophosphamide (CYP). Sodium 2-mercaptoethanesulfonate (Mesna) has been used to prevent the occurrence of HE, although this compound is not effective in established lesions. It has been demonstrated that the purinergic system is involved in several pathophysiological events. Among purinergic receptors, P2X7 deserves attention because it is involved in HE induced by CYP and, therefore, can be considered a therapeutic target. The objective of this study was to investigate the potential therapeutic effect of the quinovic acid glycosides purified fraction (QAPF) from U. tomentosa in the mouse model of CYP-induced HE. Pretreatment with QAPF not only had a protective effect on HE-induced urothelial damage (edema, hemorrhage and bladder wet weight) but was also able to control visceral pain, decrease IL-1β levels and down-regulates P2X7 receptors, most likely by inhibit the neutrophils migration to the bladder. This research clearly demonstrates the promising anti-inflammatory properties of QAPF, supporting its use as complementary therapy. QAPF represents a promising therapeutic option for this pathological condition.

Uroprotective mechanisms of natural products against cyclophosphamide-induced urinary bladder toxicity: A comprehensive review

“…IL-1β and IL-6 are other pro-inflammatory cytokines involved in the process of HC induced by CYP. Elevated bladder levels of IL-1β and IL-6 following CYP were noticed along with increased MPO activity (Arafa, 2009;Dietrich et al, 2015). Acrolein led to induction of the inflammatory cytokines through NF-kB activation, resulting in the production of cytokines like TNF-α and IL-6 as well as ROS (Keles et al, 2014;Wang et al, 2012).…”

“…The quinovic acid glycosides purified fraction (QAPF) derived from Uncaria tomentosa (F: Rubiaceae) bark and roots, which have been utilized by the Asháninkas people for various diseases (Keplinger et al, 1998), was reported to have a uroprotective action against CYP-induced HC (Dietrich et al, 2015). A marked reduction in bladder IL-1 and MPO levels was observed with QAPF treatment (Dietrich et al, 2015). Furthermore, the Caesalpinia pyramidalis Tul.…”

Delayed decompression exacerbates ischemia-reperfusion injury in cervical compressive myelopathy

“…A 0.4 g von Frey hair monofilament applied 10 times was used to measure the frequency of withdrawal to each forepaw and hind limb, where each withdrawal was measured as 10% of response (75).…”

Herbal approach in the treatment of pancytopenia

“…-Garlic [41][42][43][44][45][46] -Astragalus [47][48][49][50][51][52][53] -Uncaria tomentosa [54][55][56][57][58][59][60] Garlic: Allium sativum commonly known as garlic is generally known for its anti-microbial [41] and antihyperlipidaemic [42,43] activity. It is under research for cancer and is meta-analysed for the same [44].…”

“…They are currently researched for anti-inflammatory [58], anti-oxidant [58], denture stomatitis [59], haemorrhagic cystitis [60] and anti-fungal activity [61]. It has also claimed to have a leucocytopenic activity, especially the elevation of monocytes.…”