The quinoprotein dehydrogenases for methanol and glucose

Anthony, Christopher

doi:10.1016/j.abb.2004.03.038

Cited by 164 publications

(135 citation statements)

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“…This MD derived structure is not in accord with the following: (i) the suggestion that Asp-297-CO 2 Ϫ serves as the general-base catalyst (14)(15)(16)(17); and (ii) that the oxidation takes place, not by hydride transfer, but by an addition elimination reaction (18,19) (Scheme 1, in which the general base may be Glu-171-CO 2 Ϫ or Asp-297-CO 2 Ϫ ). The MD studies (6,7,9) showed that Asp-297-CO 2 Ϫ is not in position to act as a generalbase catalyst for the oxidation of MeOH by MDH.…”

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confidence: 87%

Mechanism of methanol oxidation by quinoprotein methanol dehydrogenase

Zhang

Reddy

Bruice

2007

Proc. Natl. Acad. Sci. U.S.A.

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hydride transfer ͉ molecular dynamics ͉ pyrroloquinoline quinone W e have had a long standing interest in the mechanism of o-quinone cofactor and model reactions (for examples, see refs. 1-4). More recently, our attention has been directed to the employment of computational methods, particularly in the mechanism of the oxidation of methanol and glucose by the appropriate quinoprotein enzymes (5-11).The present study concerns bacterial quinoprotein methanol dehydrogenase (MDH) catalysis of the oxidation of CH 3 OH 3 CH 2 O by the pyrroloquinoline quinone (PQQ) cofactor. MDH is a hetrotetramer with two heavy and two light subunits. The catalytic chemistry is located in the heavy subunits. The crystal structure (PDB code 1G72; ref. 10) of the reduced PQQ bound to MDH presents Wat1 hydrogen bonding to the -CO 2

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confidence: 87%

Mechanism of methanol oxidation by quinoprotein methanol dehydrogenase

Zhang

Reddy

Bruice

2007

Proc. Natl. Acad. Sci. U.S.A.

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“…These acids are produced in the periplasm of many Gram-negative bacteria through a direct oxidation pathway of glucose (DOPG, non-phosphorylating oxidation) whose physiological role remains uncertain (Anthony, 2001(Anthony, , 2004 Matsushita et al, 2002). The enzymes of the DOPG, the quinoproteins glucose dehydrogenase (GDH) and gluconate dehydrogenase (GADH), are oriented to the outer face of the cytoplasmic membrane so that they oxidize their substrates in the periplasmic space (Anthony, 2004). Consequently, the organic acids diffuse freely outside the cells and may release high amounts of soluble phosphorus (P) from mineral phosphates, by supplying both protons and metal complexing organic acid anions (Gadd, 1999).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region

Pérez

Sulbarán

Ball

et al. 2007

Soil Biology and Biochemistry

274

113

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With the aim to explore the possible role of mineral phosphate-solubilizing bacteria (PSB) in phosphorus (P) cycling in iron-rich, acidic soils, we conducted a survey of PSB naturally colonizing a limonitic crust in the south-east region of Venezuela (Bolı´var State). A total of 130 heterotrophic bacterial isolates showing different degrees of mineral tri-calcium phosphate (Ca 3 (PO 4 ) 2 )-solubilizing activities were isolated from NBRIP plates. In contrast, no isolates showing iron phosphate (FePO 4 )-or aluminum phosphate (AlPO 4 )-solubilizing activities were detected by this experimental approach. The 10 best Ca 3 (PO 4 ) 2 -solubilizers were selected for further characterization. These isolates were shown to belong to the genera Burkholderia, Serratia, Ralstonia and Pantoea by partial sequencing analysis of their respective 16S rRNA genes. All the PSB isolates were able to mediate almost complete solubilization of Ca 3 (PO 4 ) 2 in liquid cultures; in contrast, the PSB isolates were less effective when solubilizing FePO 4 . Two groups of PSB isolates were clearly differentiated on the basis of their Ca 3 (PO 4 ) 2 solubilization kinetics. Acidification of culture supernatants seemed to be the main mechanism for P solubilization. Indeed, gluconic acid was shown to be present in the supernatant of five isolates. Furthermore, detection of genes involved in the production of this organic acid was possible in three isolates by means of a PCR protocol. r

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“…PQQ biosynthetic pathway PQQ being synthesized from peptide containing tyrosine and glutamic acid; Fe, Zn 2+ , Ca2+ metal ions, Tyr and Glu residues [13,14] 18. Ras signaling pathway Cell protection against NO induced inhibition of cell proliferation, promoting DNA synthesis [12] …”

Section: 5-dkg Pathwaymentioning

confidence: 99%

“…PQQ synthesizing operon containing 6-7 genes i.e. (pqqABCDEF/G) [11,12] and orientation effects [13] and biocatalysis are carried by bioelectric oxidation of glucose into gluconic acid [14]. It acts as potent microbial growth stimulant and diversified role in antibiotics as chief biological control determinant for plant pathogens [15].…”

Section: Introductionmentioning

confidence: 99%

The Life History of Pyrroloquinoline Quinone (PQQ): A Versatile Molecule with Novel Impacts on Living Systems

Naveed¹

2016

IJMBOA

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Pyrroloquinoline quinone (PQQ) acting as redox cofactor of Glucose dehydrogenase is orthocyclic antioxidant acting under multifarious environmental stress and rich conditions in prokaryotes as well as eukaryotes. Microbes are the exclusive source of PQQ biosynthesis and for biocatalysis of glucose into gluconic acid and 2-ketogluconic acid by gram positive and negative bacteria. This study focus to describe PQQ biography highlighted its applications acts as biocatalyst by enhanced production of NADH from pqqC, involved in several important mechanisms like bacterial energy transduction by m-ATPase, production of biocontrol substances, growth stimulating activities and DNA repair. PQQ acting as anti-neurological, anti-degenerative, anti-melanogenic and anti-cancer agent due to its antioxidant nature by scavenging free radicals. PQQ is modulator of immunity by CD4 cells count and IL-2, sleep maintenance by PGC-1 alpha pathway and inflammation due to ROS. The mineral phosphate solubilization results in plant growth promotional, biocontrol, antifungal and ISR activities. PQQ nanoparticles used for production of Biofuels cells and sulfonated polymers. It acts as a modulator of diverse signaling pathways like STAT, MAPK, JAK, JNK, P13K/Akt, mTOR, EGFR and Raps for cell proliferation, differentiation, apoptosis, Box translocation and metabolic pathways by phosphorylation of ADP and suppression of reactive oxygen species. It protects from oxidative stress by acting as Alpha AA modulator of lysine metabolism, TrxR1 in selenium metabolism, low density lipoprotein in lipid metabolism, ATP production in Energy, Glucose and carbohydrate metabolism. It has been structurally characterized with bioinformatics tools and future perspectives being molecular modeling and docking for analytical drug design.

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The quinoprotein dehydrogenases for methanol and glucose

Cited by 164 publications

References 41 publications

Mechanism of methanol oxidation by quinoprotein methanol dehydrogenase

Mechanism of methanol oxidation by quinoprotein methanol dehydrogenase

Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region

The Life History of Pyrroloquinoline Quinone (PQQ): A Versatile Molecule with Novel Impacts on Living Systems

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