The Quantitative Analysis of Glucosinolates in Cruciferous Vegetables, Oilseeds and Forage Crops Using High Performance Liquid Chromatography

Spinks, E. Ann; Sones, Karen; Fenwick, G. R.

doi:10.1002/lipi.19840860603

Cited by 133 publications

(45 citation statements)

References 18 publications

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“…The extracts were combined to give a total volume of 10 ml and an aliquot (3 ml) was evaporated to dryness and taken up in water (3 ml) of which 2 ml was applied to small columns of DEAE Sephadex A25 and the absorbed glucosinolates desulphated as described by Heaney and Fenwick (1980). Desulphoglucosinolates were eluted with water and analysed using the high-performance liquid chromatography (HPLC) procedure described by Spinks et al (1984). The e †ect of temperature on glucosinolate concentration in both the leaves and the roots was assessed using an analysis of variance and a correlation analysis was performed between both parts of the plant.…”

Section: Methodsmentioning

confidence: 99%

Daily Variation in Glucosinolate Concentrations in the Leaves and Roots of Cabbage Seedlings in Two Constant Temperature Regimes

Rosa

1997

J. Sci. Food Agric.

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Limited information is available on the glucosinolate variation within the Brassica plant and the relationship between the pattern and concentration of glucosinolates in the aerial parts and the roots has received little attention. Early studies carried out under Ðeld conditions have shown that glucosinolate levels may vary considerably throughout a 24 h period. The purpose of the present study was to show whether, under controlled conditions, temperature was a factor in glucosinolate variation and to determine whether such variation might be due to translocation of glucosinolates between the aerial parts of the plant and its roots. Cabbage seedlings were maintained at 20 and 30¡C over 2 days and leaves and roots sampled at 02 : 00 h, 06 : 00 h, 10 : 00 h, 14 : 00 h, 18 : 00 h and 22 : 00 h. The glucosinolates 2-propenyl-and 3-methylsulphinylpropyl-with an average of 261 and 167 kmol 100 g~1 DW, respectively, were the two main glucosinolates in the aerial part of the plant whilst in the roots 1-methoxyindol-3-ylmethyl-, 2-phenylethyl-and 3-methylsulphinylpropyl, with 495, 495 and 385 kmol 100 g~1 DW respectively, showed the highest average concentrations. Total and individual glucosinolates in the roots and in the aerial part of the plant showed the highest concentrations in the dark cycle, at 02 : 00 h and 22 : 00 h, respectively, whilst the lowest levels were during the light cycle, mainly at 18 : 00 h. The results suggest that temperature was not a major factor in the short-term variation in glucosinolate levels. Although there was a very high sig-niÐcant di †erence between the total glucosinolate levels in the aerial part of the plant (581 kmol 100 g~1 DW) and roots (2124 kmol 100 g~1 DW), the results of the present study do not support the concept of translocation between aerial part and roots, suggesting that other factors may be involved.

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Section: Methodsmentioning

confidence: 99%

Daily Variation in Glucosinolate Concentrations in the Leaves and Roots of Cabbage Seedlings in Two Constant Temperature Regimes

Rosa

1997

J. Sci. Food Agric.

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“…Minchinton et al (25), Spinks et al (26), and Sang Estimates of average per person daily intake of glu-and Truscott (27), but were still subject to difficulties cosinolates range from about 16 mg in Canada (11) in interpretation because of concerns over the effects to 30 mg in the United Kingdom (12) and 112 mg of pH, time, and enzyme concentration on desulfation in Japan (13). Knowledge of the chemical types and products.…”

Section: R©cmentioning

confidence: 97%

Comprehensive Chromatographic and Spectroscopic Methods for the Separation and Identification of Intact Glucosinolates

Prestera

Fahey

Holtzclaw

et al. 1996

Analytical Biochemistry

125

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“…Hemicelluloses were calculated as NDF-ADF difference and cellulose as ADF-ADL one (Rinne et al, 1997). The GSL in the RSM calculated with the HPLC method (ISO 9167-1) by the use of Hewlett Packard chromatograph-Type 1050 apparatus (Spinks et al, 1984). Samples of rapeseed meal, diets, faeces and urine were analysed for gross energy (GE) via an adiabatic bomb calorimeter (Parr Instruments, Moline, IL, UAS).…”

Section: Measurements and Analytical Methodsmentioning

confidence: 99%

Digestibility and nitrogen balance of pig diets containing gradually increasing levels of rapeseed meal

Kargopoulos

Dotas²,

Nikolakakis³

et al. 2018

J Hellenic Vet Med Soc

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Rapeseed meal (RSM), a by-product of extracted rapeseed oil production, is a potential protein source for use in pig diets. To determine the best levels of RSM inclusion in diets of fattening pigs as well as the digestibility and energy value of diets containing gradually increasing levels of RSM, a digestibility trial was conducted. The trial was performed according to a 4x4 Latin square experimental design, with 4 Large White x Landrace castrated male pigs weighing on average 47.3kg. The animals were housed in metabolism cages during the whole period the duration of which was a total of 66 days. The experiment was divided into 5 periods, an adjustment period and 4 experimental periods, during which pigs consumed 4 experimental diets. The diet C (control) was a basal diet based on corn and soybean meal, while the other experimental diets included RSM at levels 90 (low- L), 180 (medium- M) and 270 (high- H) g.kg-1 of feed, respectively. Each experimental period consisted of 10 preliminary days during which the animals were adapted to the diets, followed by a 4 days period for collection of faeces, urine and feed refusal. The gradual increase in the level of RSM in the experimental diets resulted in a significant (P<0.05) reduction in the apparent digestibility of CP, but only for the diet with the highest level of RSM, compared to the control diet and this one with the lowest level of RSM . Any increase in the level of RSM in the experimental diets resulted in significant (P<0.05) increase on the apparent digestibility coefficient (ADC) of CF and very significant (P<0.01) increase in diets M and H, compared to the control and diet L. Similar were the effects of RSM levels in the diets on the apparent digestibility coefficient of NDF, ADF and cellulose. As conclusion, the gradual increase in the level of RSM in the fattening pigs’ diets did not significantly affect ADC of DM and gross energy, caused a significant reduction in the ADC of CP, and a significant improvement in the ADC of CF as well.

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The Quantitative Analysis of Glucosinolates in Cruciferous Vegetables, Oilseeds and Forage Crops Using High Performance Liquid Chromatography

Cited by 133 publications

References 18 publications

Daily Variation in Glucosinolate Concentrations in the Leaves and Roots of Cabbage Seedlings in Two Constant Temperature Regimes

Daily Variation in Glucosinolate Concentrations in the Leaves and Roots of Cabbage Seedlings in Two Constant Temperature Regimes

Comprehensive Chromatographic and Spectroscopic Methods for the Separation and Identification of Intact Glucosinolates

Digestibility and nitrogen balance of pig diets containing gradually increasing levels of rapeseed meal

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