THE QUANTITATIVE ANALYSIS OF CHLOROPHYLLS a AND b IN PLANT EXTRACTS

Bruuinsma, Johan

doi:10.1111/j.1751-1097.1963.tb08220.x

Cited by 527 publications

(204 citation statements)

References 13 publications

(3 reference statements)

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“…Chlorophyll concentrations from each replicate (3 per treatment) were determined according to Bruinsma (1963). One gram of fresh leaf tissue was submerged into 100 mL of 95% ethanol and kept in dark at room temperature until the tissue was discolored; the readings were taken at 649 nm and 665 nm.…”

Section: Leaf Protein and Chlorophyll Analysesmentioning

confidence: 99%

Effects of AM colonization on “wild tobacco” plants grown in zinc-contaminated soil

Audet

Charest

2006

Mycorrhiza

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This greenhouse study aimed to determine the effect of colonization by the arbuscular mycorrhizal (AM) fungus (Glomus intraradices Schenck & Smith) on the 'wild' tobacco (Nicotiana rustica L. var. Azteca), under soil-zinc (Zn) conditions. Plants of N. rustica were grown in AM or non-AM inoculated substrate and subjected to four soil- [Zn] concentrations (0, 50, 100, and 250mg Zn kg -1 dry soil). The AM root colonization increased markedly from 14% to 81% with the increasing soil-[Zn] and the mycorrhizal structures were significantly more abundant at the highest soil- [Zn], suggesting that Zn may be involved directly or indirectly in AM root colonization. In addition, total Zn content or Zn concentrations in shoots and roots were shown to increase as soil-[Zn] increased in both AM and non-AM plants. As for the growth parameters studied, there were no significant differences between treatments despite the increase in Zn content or concentration. The AM roots subjected to the highest soil-[Zn] had a significant reduction by about 50% of total Zn content and Zn concentration compared to non-AM roots.The percent Zn extracted in shoots and roots decreased as soil- [Zn] increased, while the extracted-Zn % was lower in AM treatments for shoots at 100- [Zn] and roots at 50- [Zn]. SoilpH was significantly lower in non-AM than AM treatments at the highest soil- [Zn]. In summary, AM plants (particularly roots) showed lower Zn content and concentration than non-AM plants.In this regard, the AM fungi have a protective role for the host host plant, thus playing an important role in soil-contaminated immobilization processes; and, therefore, be of value in phytoremediation, especially when heavy metals approach toxic levels in the soil.

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Section: Leaf Protein and Chlorophyll Analysesmentioning

confidence: 99%

Effects of AM colonization on “wild tobacco” plants grown in zinc-contaminated soil

Audet

Charest

2006

Mycorrhiza

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“…SPAD readings reflect the greenness of the leaves and are an indirect measurement of chlorophyll content. As reference, chlorophyll content was determined destructively on 100 leaf sections of 2 cm 2 with acetone according to Bruinsma (1963). The absorbance of extracts was evaluated at 663.6 nm (A663.6) and 646.6 nm (A646.6) with an ultraviolet-visible (UV-vis) spectrophotometer (Perkin-Elmer, Lambda 5, Massachusetts, USA) according to the equations of Porra et al (1989).…”

Section: Trait Evaluationmentioning

confidence: 99%

Relationship between survival and yield related traits in Solanum pimpinellifolium under salt stress

et al. 2012

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A subset of the Solanum pimpinellifolium collection maintained by AVRDC-The World Vegetable Center, Taiwan was evaluated to assess effects of salt stress on physiological traits and yield-related traits with the aim of identifying potential S. pimpi nellifolium accessions useful for salt tolerance breed ing in tomato. We undertook a comparative analysis of yield and plant survival traits under normal and salt stress conditions to obtain a first indication of the crucial traits associated with salt tolerance in S. pimpinellifolium. Although most traits of S. pimpinellifolium accessions showed a similar percent decrease in mean under salt stress compared with the cultivated checks, the former exhibited a wide range for all traits, suggesting great genetic diversity that can be exploited for the identification of salt tolerant genotypes. Genetic variability for yield and survival traits under salt stress was quantitative with low to moderate heritability. Results of correlation and path coefficient analysis revealed no correlation between any of the physiological traits with yield-related traits indicating that the ability to survive and yield under salt stress are two independent sets of traits in S. pimpinellifolium. Results of the path analysis along with heritability and genetic advance showed that shoot dry weight and K/Na ratio are the two most critical component traits for survival, while fruit number is critical for yield per plant. The large S. pimpinellifolium panel evaluated in this study revealed five genotypes possessing better survival traits, seven genotypes with good yield traits, and two genotypes combining both superior survival and yield traits under salt stress.

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“…Leaves were rapidly removed from the cuvette after different lengths of time at 2-0 kPa p(O 2 ), frozen in liquid nitrogen and extracted as described by Perchorowicz and Jensen (1983). Chlorophyll was estimated according to Bruinsma (1963). ATP was measured by the luciferase method using the luminometer and assay chemicals from LKB Wallac (Turku, Finland).…”

Section: Determination Of Atp and Rubp Contentmentioning

confidence: 99%

Regeneration of Ribulose 1,5-bisphosphate and Ribulose 1,5-bisphosphate carboxylase/oxygenase Activity Associated with Lack of Oxygen Inhibition of Photosynthesis at Low Temperature

1986

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Schnyder, H., Machler, F. and Nosberger, J. 1986. Regeneration of ribulose 1,5-bisphosphate and ribulose 1,5-bisphosphate carboxylase/oiygenase activity associated with lack of oxygen inhibition of photosynthesis at low temperature.-J. exp. Bot. 37: 1170-1179.The nature of the lack of oxygen inhibition of C 3 -photosynthesis at low temperature was investigated in white clover (Trifolium repens L.). Detached leaves were brought to steady-state photosynthesis in air (34 Pa p(CO 2 ), 21 kPa p(O 2 ), balance N 2 ) at temperatures of 20 °C and 8°C, respectively. Net photosynthesis, ribulose 1,5-bisphosphate (RuBP) and ATP contents, and ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activities were followed before and after changing to 2-0 kPa At 20 °C, lowering /^Oj) increased net photosynthesis by 37%. This increase corresponded closely with the increase expected from the effect on the kinetic properties of RuBPCO. Conversely, at 8 °C net photosynthesis rapidly decreased following a decrease in p(O 2 ) and then increased again reaching a steady-state level which was only 7% higher than at 21 kPa piOJ. The steady-state rates of RuBP and associated ATP consumption were both estimated to have decreased. ATP and RuBP contents decreased by 18% and 33% respectively, immediately after the change in p(O 2 ), suggesting that RuBP regeneration was reduced at low piOJ) due to reduced photophosphorylation. Subsequently, RuBP content increased again. Steady-state RuBP content at 2-0 kPa p(O 2 ) was 24% higher than at 21 kPa p(Oj). RuBPCO activity decreased by 22%, indicating control of steady-state RuBP consumption by RuBPCO activity.It is suggested that lack of oxygen inhibition of photosynthesis at low temperature is due to decreased photophosphorylation at low temperature and low p (O 2 ). This may be due to assimilate accumulation within the chloroplasts. Decreased photophosphorylation seems to decrease RuBP synthesis and RuBPCO activity, possibly due to an acidification of the chloroplast stroma.

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THE QUANTITATIVE ANALYSIS OF CHLOROPHYLLS a AND b IN PLANT EXTRACTS

Cited by 527 publications

References 13 publications

Effects of AM colonization on “wild tobacco” plants grown in zinc-contaminated soil

Effects of AM colonization on “wild tobacco” plants grown in zinc-contaminated soil

Relationship between survival and yield related traits in Solanum pimpinellifolium under salt stress

Regeneration of Ribulose 1,5-bisphosphate and Ribulose 1,5-bisphosphate carboxylase/oxygenase Activity Associated with Lack of Oxygen Inhibition of Photosynthesis at Low Temperature

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