The Q-system: A Versatile Repressible Binary Expression System

Fölsz, Orsolya; Lin, Chun-Chieh; Task, Darya; Riabinina, Olena; Potter, C. J.

doi:10.1007/978-1-0716-2541-5_2

Cited by 4 publications

(2 citation statements)

References 108 publications

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“…Some groups have ported the binary GAL4-UAS system to Aedes and Anopheles mosquito strains [54][55][56][57], but the pace of deploying these transgenic systems broadly to the rest of the mosquito research community has not been rapid. Rather, a new binary transgenic platform called the Q-system from the Potter lab was first developed in Drosophila to be orthogonal to the GAL4-UAS system and [58,59] has now been successfully applied to An. gambiae [60] and An.…”

Section: Why Think Of Fruit Flies When Mosquitoes Are Humanity's Bigg...mentioning

confidence: 99%

Transposon and Transgene Tribulations in Mosquitoes: A Perspective of piRNA Proportions

Lau,

Macias

2024

DNA

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Mosquitoes, like Drosophila, are dipterans, the order of “true flies” characterized by a single set of two wings. Drosophila are prime model organisms for biomedical research, while mosquito researchers struggle to establish robust molecular biology in these that are arguably the most dangerous vectors of human pathogens. Both insects utilize the RNA interference (RNAi) pathway to generate small RNAs to silence transposons and viruses, yet details are emerging that several RNAi features are unique to each insect family, such as how culicine mosquitoes have evolved extreme genomic feature differences connected to their unique RNAi features. A major technical difference in the molecular genetic studies of these insects is that generating stable transgenic animals are routine in Drosophila but still variable in stability in mosquitoes, despite genomic DNA-editing advances. By comparing and contrasting the differences in the RNAi pathways of Drosophila and mosquitoes, in this review we propose a hypothesis that transgene DNAs are possibly more intensely targeted by mosquito RNAi pathways and chromatin regulatory pathways than in Drosophila. We review the latest findings on mosquito RNAi pathways, which are still much less well understood than in Drosophila, and we speculate that deeper study into how mosquitoes modulate transposons and viruses with Piwi-interacting RNAs (piRNAs) will yield clues to improving transgene DNA expression stability in transgenic mosquitoes.

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Section: Why Think Of Fruit Flies When Mosquitoes Are Humanity's Bigg...mentioning

confidence: 99%

Transposon and Transgene Tribulations in Mosquitoes: A Perspective of piRNA Proportions

Lau,

Macias

2024

DNA

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“…Given that adipose tissue acts as an essential mediator of energy homeostasis in both health and disease, it is critical to gain a better understanding of the cellular and molecular mechanisms that allow adipocytes to relay physiological information to peripheral organs. The incredible genetic toolkit (Caygill and Brand, 2016; Fölsz et al, 2022; Gratz et al, 2023), presence of organ systems analogous to mammals, and high degree of genetic conservation (Ugur et al, 2016) make Drosophila melanogaster an excellent in vivo model organism to address the role that adipose tissue plays in inter-organ communication.…”

Section: Introductionmentioning

confidence: 99%

Improved whole-mount immunofluorescence protocol for consistent and robust labeling of adultDrosophila melanogasteradipose tissue

Ott,

Armstrong

2024

Preprint

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Energy storage and endocrine functions of the Drosophila fat body make it an excellent model for elucidating mechanisms that underlie physiological and pathophysiological organismal metabolism. Combined with Drosophila's robust genetic and immunofluorescence microscopy toolkits, studies of Drosophila fat body function are ripe for cell biological analysis. Unlike the larval fat body, which is easily removed as a single, cohesive sheet of tissue, isolating intact adult fat body proves to be more challenging, thus hindering consistent immunofluorescence labeling even within a single piece of adipose tissue. Here, we describe an improved approach to handling Drosophila abdomens that ensures full access of the adult fat body to solutions generally used in immunofluorescence labeling protocols. In addition, we assess the quality of fluorescence reporter expression and antibody immunoreactivity in response to variations in fixative tupe, fixation incubation time, and detergent used for cellular permeabilization. Overall, we provide several recommendations for steps in a whole mount staining protocol that results in consistent and robust immunofluorescence labeling of the adult Drosophila fat body.

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Dynamic encoding of temperature in the central circadian circuit coordinates physiological activities

Li,

Yuan

et al. 2024

Nat Commun

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The circadian clock regulates animal physiological activities. How temperature reorganizes circadian-dependent physiological activities remains elusive. Here, using in-vivo two-photon imaging with the temperature control device, we investigated the response of the Drosophila central circadian circuit to temperature variation and identified that DN1as serves as the most sensitive temperature-sensing neurons. The circadian clock gate DN1a’s diurnal temperature response. Trans-synaptic tracing, connectome analysis, and functional imaging data reveal that DN1as bidirectionally targets two circadian neuronal subsets: activity-related E cells and sleep-promoting DN3s. Specifically, behavioral data demonstrate that the DN1a-E cell circuit modulates the evening locomotion peak in response to cold temperature, while the DN1a-DN3 circuit controls the warm temperature-induced nocturnal sleep reduction. Our findings systematically and comprehensively illustrate how the central circadian circuit dynamically integrates temperature and light signals to effectively coordinate wakefulness and sleep at different times of the day, shedding light on the conserved neural mechanisms underlying temperature-regulated circadian physiology in animals.

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The Q-system: A Versatile Repressible Binary Expression System

Cited by 4 publications

References 108 publications

Transposon and Transgene Tribulations in Mosquitoes: A Perspective of piRNA Proportions

Transposon and Transgene Tribulations in Mosquitoes: A Perspective of piRNA Proportions

Improved whole-mount immunofluorescence protocol for consistent and robust labeling of adultDrosophila melanogasteradipose tissue

Dynamic encoding of temperature in the central circadian circuit coordinates physiological activities

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