The pyruvate decarboxylase 1 (PDC1) gene: negative regulator of disease resistance for Fusarium oxysporum and Verticillium dahliae

A novel strategy for improving watermelon resistance to cucumber green mottle mosaic virus by exogenous boron application

“…In our study, the expression of AKR1 and AKR2 was found to be up-regulated by exogenous boron under CGMMV infection, while silencing of AKR1 expression increased CGMMV infection and MDA content (Figure 6a-e,j). Pyruvate is a glycolytic product and is catalysed by PDC to produce acetaldehyde and then ethanol, not LA, in plants, leading to less damage and less acidification in watermelon fruits (Papastolopoulou et al, 2018). We also found that the expression of PDC1 was…”

“…In our study, the expression of AKR1 and AKR2 was found to be up‐regulated by exogenous boron under CGMMV infection, while silencing of AKR1 expression increased CGMMV infection and MDA content (Figure 6a–e,j ). Pyruvate is a glycolytic product and is catalysed by PDC to produce acetaldehyde and then ethanol, not LA, in plants, leading to less damage and less acidification in watermelon fruits (Papastolopoulou et al, 2018 ). We also found that the expression of PDC1 was significantly down‐regulated in the C + H vs. P + H comparison and up‐regulated in the C + B vs. C + H comparison in the late fruit growth stage, and silencing of PDC1 expression through VIGS enhanced CGMMV infection, increased pyruvic acid and LA contents, but reduced ethanol content in watermelon fruits (Figure 6a–e,k ).…”

Adding by subtracting : Impairing tomato susceptibility genes to obtain resistance to Verticillium wilt

“…Nevertheless, the use of impaired S genes represents a major opportunity 42 | Chapter 3 for resistance breeding, especially in cases where qualitative resistances are insufficient or not available. For V. dahliae, only two S genes have been described in literature, namely Walls are thin 1 (WAT1) and Pyruvate decarboxylase 1 (PDC1), but these studies were carried out in Arabidopsis thaliana (Denancé et al, 2013;Papastolopoulou et al, 2018), whereas their role as S gene in crop species such as tomato remains unclear.…”

“…A second interesting candidate from Arabidopsis is pyruvate decarboxylase 1 (PDC1) which is a negative regulator of disease resistance against vascular wilt fungi V. dahliae and Fusarium oxysporum. A lower percentage of diseased leaves and reduced fungal biomass were found for the pdc1 mutant for both fungi (Papastolopoulou et al, 2018). PDC1 is an enzyme that catalyses the decarboxylation of pyruvic acid to acetaldehyde and carbon dioxide (Kursteiner et al, 2003;Mithran et al, 2014), but its role in plant immunity remains to be elucidated.…”