The PVT1, HULC, and HOTTIP expression changes due to treatment in Diffuse Large B-cell lymphoma

Abstract

Diffuse large B-cell lymphoma is the most common histological subtype of non-Hodgkin’s lymphomas. It is an aggressive malignancy that displays great heterogeneity in morphology, genetics, biological behavior and treatment response owing to chromatin remodeling and epigenetics.Bioinformatic-based approaches were used to understand the possible signaling pathways of the three lncRNAsPVT1,HULC, andHOTTIP. Furthermore, their expression levels were quantitatively evaluated in 100 patients before and after the treatment.The results revealed that gene expression was significantly upregulated inPVT1,HULC, andHOTTIPby 7.39±8.48-, 5.924±7.536-, and 4.137±5.863 fold, respectively, relative to normal cases. Post-treatment measurement of lncRNA expression indicated thatPVT1andHOTTIPwere significantly downregulated.Interestingly, the expression levels ofPVT1,HULC, andHOTTIPwere significantly higher in DLBCL patients aged > 60 years than in those aged < 60 years. In addition, there was a significant positive correlation betweenHULCandHOTTIPexpression.The analysis of overexpressed lncRNA-miRNA interaction indicated different deregulated miRNA targets and the protein targets of upregulated lncRNAs are mainly with histone modification, DNA methylation/demethylation, and protein methyltransferase activity.Summary blurbThe lncRNAsPVT1,HULC, andHOTTIPexpression is significantly upregulated before treatment and reduce to normal level after treatment. It can be used as diagnostic marker or prognostic means especially in Relapsed/refractory DLBCL.