The Purification and Properties of Platelet Cofactor I (Factor VIII)

Mammen, E.F.

doi:10.1159/000460765

Cited by 1 publication

(1 citation statement)

References 16 publications

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“…I n human plasma the assay gave from 6 to 330 units of platelet cofactor I per milliliter of plasma with an average of 162 units (36). In bovine plasma it was between 50 and 200 units per milliliter plasma, the average was 116 units (23). With modified thromboplastin generation tests values were found, that ranged between 50 and 200% for normal human plasma (6,29,30).…”

Section: Discussionmentioning

confidence: 93%

Concentration of Platelet Cofactor I (Factor VIII) from Bovine Serum*

Mammen¹

1964

Thromb Haemost

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SummaryA method for obtaining concentrates of platelet cofactor I (factor VIII) from ether extracted bovine serum has been developed. Platelet cofactor I is inactivated during the coagulation process by undergoing a complex formation, which is broken by ether extraction.In each of 13 preparations from 1,000 ml of extracted serum an average total activity of 3,055 platelet cofactor I units was isolated. The specific activity was 4,760 units per milligram tyrosine and 138 units per milligram protein (Biuret.)In the paper electrophoresis the fractions were homogeneous. Using immunoelectrophoretic techniques three bands were found against anti-bovine serum from rabbits. The preparations activated purified prothrombin in the presence of platelets, Ac-globulin and calcium ions. They substituted in the thromboplastin generation test for adsorbed plasma and corrected the delayed thromboplastin generation of hemophilia A plasma. They also corrected the prolonged recalcification times of hemophilia A plasma. The preparations lost their activity upon storage at room temperature and in the icebox rapidly. Also freeze drying resulted in a complete loss of activity.

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Section: Discussionmentioning

confidence: 93%

Concentration of Platelet Cofactor I (Factor VIII) from Bovine Serum*

Mammen¹

1964

Thromb Haemost

View full text Add to dashboard Cite

show abstract

The Purification and Properties of Platelet Cofactor I (Factor VIII)

Cited by 1 publication

References 16 publications

Concentration of Platelet Cofactor I (Factor VIII) from Bovine Serum*

Concentration of Platelet Cofactor I (Factor VIII) from Bovine Serum*

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