The purification and properties of aldose reductase from rat ovary

Iwata, Nobuhisa; Inazu, Norihisa; Satoh, Takaya

doi:10.1016/0003-9861(90)90088-g

Cited by 28 publications

(8 citation statements)

References 37 publications

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“…AR is expressed in rat granulosa cells and oocytes, and SDH is highly expressed in the oocyte (Iwata et al 1990, Kaneko et al 2003. While cumulus cells have high levels of glycolytic activity to provide the oocyte with metabolites for oxidative phosphorylation (Sutton et al 2003b), localisation of both enzymes suggests that granulosa cells convert glucose to sorbitol, providing the oocyte with alternative substrates for energy production, namely fructose.…”

Section: Polyol Pathwaymentioning

confidence: 99%

The pivotal role of glucose metabolism in determining oocyte developmental competence

Sutton‐McDowall

Gilchrist²,

Thompson³

2010

Reproduction

427

325

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The environment that the cumulus oocyte complex (COC) is exposed to during either in vivo or in vitro maturation (IVM) can have profound effects on the success of fertilisation and subsequent embryo development. Glucose is a pivotal metabolite for the COC and is metabolised by glycolysis, the pentose phosphate pathway (PPP), the hexosamine biosynthesis pathway (HBP) and the polyol pathway. Over the course of oocyte maturation, a large proportion of total glucose is metabolised via the glycolytic pathway to provide substrates such as pyruvate for energy production. Glucose is also the substrate for many cellular functions during oocyte maturation, including regulation of nuclear maturation and redox state via the PPP and for the synthesis of substrates of extracellular matrices (cumulus expansion) and O-linked glycosylation (cell signalling) via the HBP. However, the oocyte is susceptible to glucose concentration-dependent perturbations in nuclear and cytoplasmic maturation, leading to poor embryonic development post-fertilisation. For example, glucose concentrations either too high or too low result in precocious resumption of nuclear maturation. This review will discuss the relevant pathways of glucose metabolism by COCs during in vivo maturation and IVM, including the relative contribution of the somatic and gamete compartments of the COC to glucose metabolism. The consequences of exposing COCs to abnormal glucose concentrations will also be examined, either during IVM or by altered maternal environments, such as during hyperglycaemia induced by diabetes and obesity.Reproduction (2010) 139 685-695

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Section: Polyol Pathwaymentioning

confidence: 99%

The pivotal role of glucose metabolism in determining oocyte developmental competence

Sutton‐McDowall

Gilchrist²,

Thompson³

2010

Reproduction

427

325

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“…AR is a ubiquitous "housekeeping" enzyme probably functional in all cells (9,10). An osmoregulatory role of AR has been suggested by studies in cell lines derived from renal inner medulla showing that an increase in the osmolality of the medium is associated with increases in cellular sorbitol levels, AR activity, and AR gene expression (3,4,11).…”

Section: For Review)mentioning

confidence: 99%

Isolation of the Mouse Aldose Reductase Promoter and Identification of a Tonicity-responsive Element

Daoudal

Tournaire

Halere

et al. 1997

Journal of Biological Chemistry

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Aldose reductase (AR; EC 1.1.1.21) is an oxidoreductase that catalyzes the NADPH-dependent conversion of glucose to sorbitol, the first step of the polyol pathway. AR is of great interest due to its implication in the etiology of diabetic complications. In renal medullary cells, AR also plays an osmoregulatory role by accumulating sorbitol to maintain the intracellular osmotic balance during antidiuresis. We have previously cloned the AR cDNA from mouse kidney, and we report here the isolation of the mouse AR gene promoter. Transient transfection of chloramphenicol acetyltransferase reporter constructs containing various 5-flanking regions of the mouse AR gene in CV1 cells led to the identification of a sequence spanning base pairs ؊1053 to ؊1040, required for an enhancer activity in hypertonic compared with isotonic cell culture conditions. This sequence is similar to the tonicity-responsive element first characterized in the betaine-␥-aminobutyric acid transporter promoter.

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“…In this study, the effect of DS on PNBA reductase activity was different from other reductase activities tested. Recently, we have purified aldose reductase from rat ovary and demonstrated that ovarian aldose reduc tase shows higher affinity for PNBA than ova rian CR (23). Thus, PNBA reductase activity in rat ovary may depend on aldose reductase and aldehyde reductase rather than CR.…”

Section: Discussionmentioning

confidence: 99%

Consecutive Treatment of Disulfiram Inhibits Ovarian Carbonyl Reductase Activity in Rats.

1992

Self Cite

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We investigated the effects of disulfiram (DS) on ovarian carbonyl re ductase activity in rats to determine the influence of DS on female reproductive func tion. Three consecutive treatments with DS significantly inhibited ovarian carbonyl re ductase activity as well as ovulation, dose-dependently. Single treatment with DS had no effect on ovarian carbonyl reductase activity. Our observations indicate that con secutive treatment with DS has an inhibitory action on female reproductive function, although DS is well-known to inhibit aldehyde dehydrogenase and dopamine ,8-hy droxylase.

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The purification and properties of aldose reductase from rat ovary

Cited by 28 publications

References 37 publications

The pivotal role of glucose metabolism in determining oocyte developmental competence

The pivotal role of glucose metabolism in determining oocyte developmental competence

Isolation of the Mouse Aldose Reductase Promoter and Identification of a Tonicity-responsive Element

Consecutive Treatment of Disulfiram Inhibits Ovarian Carbonyl Reductase Activity in Rats.

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