The PRP8 inteins in Cryptococcus are a source of phylogenetic and epidemiological information

Butler, Margaret I.; Poulter, Russell T. M.

doi:10.1016/j.fgb.2005.01.011

Cited by 43 publications

(49 citation statements)

References 52 publications

(96 reference statements)

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“…Inteins, known as parasitic genetic elements, may prove to be a promising resource for differentiating related species (37,38) since they are located in highly conserved genes. Inteins are intervening sequences that are transcribed and translated with flanking host protein sequences and then are self-excised by protein splic-ing; the flanking protein sequences (exteins) are joined by a peptide bond to form the functional protein (39)(40)(41).…”

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Analysis of Inteins in the Candida parapsilosis Complex for Simple and Accurate Species Identification

et al. 2013

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cInteins are coding sequences that are transcribed and translated with flanking sequences and then are excised by an autocatalytic process. There are two types of inteins in fungi, mini-inteins and full-length inteins, both of which present a splicing domain containing well-conserved amino acid sequences. Full-length inteins also present a homing endonuclease domain that makes the intein a mobile genetic element. These parasitic genetic elements are located in highly conserved genes and may allow for the differentiation of closely related species of the Candida parapsilosis (psilosis) complex. The correct identification of the three psilosis complex species C. parapsilosis, Candida metapsilosis, and Candida orthopsilosis is very important in the clinical setting for improving antifungal therapy and patient care. In this work, we analyzed inteins that are present in the vacuolar ATPase gene VMA and in the threonyl-tRNA synthetase gene ThrRS in 85 strains of the Candida psilosis complex (46 C. parapsilosis, 17 C. metapsilosis, and 22 C. orthopsilosis). Here, we describe an accessible and accurate technique based on a single PCR that is able to differentiate the psilosis complex based on the VMA intein. Although the ThrRS intein does not distinguish the three species of the psilosis complex by PCR product size, it can differentiate them by sequencing and phylogenetic analysis. Furthermore, this intein is unusually present as both mini-and full-length forms in C. orthopsilosis. Additional population studies should be performed to address whether this represents a common intraspecific variability or the presence of subspecies within C. orthopsilosis.

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Analysis of Inteins in the Candida parapsilosis Complex for Simple and Accurate Species Identification

et al. 2013

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“…The PRP8 intein was initially described in a large number of C. neoformans (varieties grubii and neoformans) and C. gattii isolates as mini-inteins (without the HEG domain) (Butler et al 2001, Butler & Poulter 2005. Besides these Cryptococcus species, the PRP8 intein can also be found as a full-length intein in Cryptococcus laurentii.…”

Section: Prp8 Inteinmentioning

confidence: 99%

“…Besides these Cryptococcus species, the PRP8 intein can also be found as a full-length intein in Cryptococcus laurentii. Since this species is moderately distant from C. neoformans, Butler & Poulter (2005) suggested that the mini-intein found in C. neoformans and C. gattii was derived from horizontal transfer, with C. laurentii or another species being the donor. Butler et al (2006) searched for PRP8 inteins in a database of genomes and discovered it in many species of Ascomycetes.…”

Section: Prp8 Inteinmentioning

confidence: 99%

“…In this group, however, there is no evidence of horizontal transfer, since the phylogenetic analysis of these inteins is similar to those using other nuclear sequences, for example, ribosomal sequences, which suggests an ancient origin and vertical transmission among Euascomycetes (Butler et al 2006). Since only a few Cryptococcus species (Basidiomycota, order Tremalles) present the PRP8 intein, of which C. laurentii is the only one with a full-length intein (Butler & Poulter 2005) and no other basidiomycetes have been shown to have inteins, it seems reasonable to explain this distribution of the PRP8 intein as the result of horizontal transfer between ascomycetes (donors) and basidiomycetes (receptors). This transfer, according to Poulter et al (2007), could have occurred during the parasitic phase, in co-infection cases, or during the saprobic phase of these pathogens, when different fungal cells are phagocytosed by microorganisms from soil.…”

Section: Prp8 Inteinmentioning

confidence: 99%

“…Using this approach, Butler and Poulter (2005) distinguished between strains of two varieties of C. neoformans (neoformans and grubii) and from strains of C. gattii (recently designated as a new species) by the neighbour joining method. These encapsulated yeasts are capable of causing serious infections in both immunocompromised and immunocompetent persons.…”

Section: Species Concept and Use Of Prp8 Intein For Phylogenetic Studmentioning

confidence: 99%

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Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications

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Current awareness on yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 17th. Aug. 2005)

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The PRP8 inteins in Cryptococcus are a source of phylogenetic and epidemiological information

Cited by 43 publications

References 52 publications

Analysis of Inteins in the Candida parapsilosis Complex for Simple and Accurate Species Identification

Analysis of Inteins in the Candida parapsilosis Complex for Simple and Accurate Species Identification

Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications

Current awareness on yeast

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