The protozoan parasites

Leiby, David A.; Wendel, Silvano

doi:10.1017/cbo9780511545245.012

Cited by 1 publication

(1 citation statement)

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“…Formerly, the authors assumed that positive IgM anti‐ Toxoplasma antibodies by ELISA means acute infection, but it should be considered that IgM start to rise in acute infection and then decreased at convalescent phase by a very variable manner, which might be persisted for months and even more. Thus, it is difficult to diagnose acute infection even IgM positivity was established by a Toxoplasma reference laboratory as three likelihoods might be considered: a recent infection, past infection, and a false positive result . Also, anti‐toxoplasmosis IgG antibodies which are present at convalescent phase and maintained lifetime, does not indicated acute infection or risk of parasitemia.…”

Section: Discussionmentioning

confidence: 99%

Serological and molecular screening of umbilical cord blood for Toxoplasma gondii infection

Botein

Darwish

El-Tantawy

et al. 2019

Transplant Infectious Dis

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Background: Human umbilical cord blood has proven to be a successful alternate source of hematopoietic stem cells for pediatric patients with major hematologic disorders. Toxoplasma gondii is a global opportunistic protozoan which cause fatal complications in immunocompromised individuals. Aim:Our goal is to study the prevalence of toxoplasmosis in umbilical cord blood (UCB) and to assess the sensitivity of ELISA and PCR for Toxoplasma infection screening. Material and Methods:One hundred cord blood samples were collected immediately after delivery. Anti-Toxoplasma IgG and IgM antibodies were determined using ELISA method; Toxoplasma DNA was detected using nested PCR technique. Total nucleated cells (TNC) and HB were also determined. Demographic data and risk factors data related to the transmission of toxoplasmosis, were collected from mothers. Results:Among 100 cord blood samples, 36 (36%) were positive for anti-Toxoplasma IgG antibodies and 6 (6%) were positive for anti-Toxoplasma IgM antibodies. The nested PCR showed 11 (11%) samples containing Toxoplasma DNA from which, 6 (55%) samples were IgM positive. There was no significant association between the risk of Toxoplasma transmission and cord blood positivity for toxoplasmosis. Conclusion:Owing to the prevalence of toxoplasmosis, its rapid progression and its fatal outcome in immunocompromised patients, cord blood screening for toxoplasmosis with nested PCR should be incorporated into cord blood bank screening protocols. K E Y W O R D Sbank, cord blood, diagnosis, screening, Toxoplasma

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Section: Discussionmentioning

confidence: 99%