The Proto-oncogene Transcription Factor Ets1 Regulates Neural Crest Development through Histone Deacetylase 1 to Mediate Output of Bone Morphogenetic Protein Signaling

Wang, Cheng Dong; Kam, Richard Kin Ting; Shi, Weili; Xia, Yin; Chen, Xiongfong; Cao, Ying; Sun, Jianmin; Du, Yanzhi; Lü, Gang; Chen, Zi‐Jiang; Chan, Wood Yee; Chan, Sun On; Deng, Yi; Zhao, Hui

doi:10.1074/jbc.m115.644864

Cited by 35 publications

(43 citation statements)

References 59 publications

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“…HDACs have also been linked to the regulation of neural crest transcription, migration and differentiation. For example, Hdac1 has been shown to physically interact with Ets1 to control the expression of id3, a BMP target gene in Xenopus laevis (Wang et al, 2015). Similarly, Snail2 has been shown to recruit the HDAC-Sin3A complex to repress Cad6B expression, suggesting that HDAC activity is important for epithelial-mesenchymal transition (EMT) and migration (Strobl-Mazzulla and Bronner, 2012).…”

Section: Discussionmentioning

confidence: 99%

Histone deacetylase activity has an essential role in establishing and maintaining the vertebrate neural crest

Rao

LaBonne

2018

Development

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The neural crest, a progenitor population that drove vertebrate evolution, retains the broad developmental potential of the blastula cells it is derived from, even as neighboring cells undergo lineage restriction. The mechanisms that enable these cells to preserve their developmental potential remain poorly understood. Here, we explore the role of histone deacetylase (HDAC) activity in this process in We show that HDAC activity is essential for the formation of neural crest, as well as for proper patterning of the early ectoderm. The requirement for HDAC activity initiates in naïve blastula cells; HDAC inhibition causes loss of pluripotency gene expression and blocks the ability of blastula stem cells to contribute to lineages of the three embryonic germ layers. We find that pluripotent naïve blastula cells and neural crest cells are both characterized by low levels of histone acetylation, and show that increasing HDAC1 levels enhance the ability of blastula cells to be reprogrammed to a neural crest state. Together, these findings elucidate a previously uncharacterized role for HDAC activity in establishing the neural crest stem cell state.

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Section: Discussionmentioning

confidence: 99%

Histone deacetylase activity has an essential role in establishing and maintaining the vertebrate neural crest

Rao

LaBonne

2018

Development

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“…The preparation of digoxigenin-labeled RNA probe and whole mount in situ hybridization were performed as described previously (Shi et al, 2015; Wang et al, 2015). Embryos probed with indicated antisense RNAs were sectioned by a vibratome (Zeiss) to a thickness of 50 μm (Shi et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest

Wong¹,

Rebbert²,

Wang³

et al. 2016

Int. J. Dev. Biol.

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Neural crest (NC) development is controlled precisely by a regulatory network with multiple signaling pathways and the involvement of many genes. The integration and coordination of these factors are still incompletely understood. Overexpression of Wnt3a and the BMP antagonist Chordin in animal cap cells from Xenopus blastulae induces a large number of NC specific genes. We previously suggested that Potassium Channel Tetramerization Domain containing 15 (Kctd15) regulates NC formation by affecting Wnt signaling and the activity of transcription factor AP-2. In order to advance understanding of the function of Kctd15 during NC development, we performed DNA microarray assays in explants injected with Wnt3a and Chordin, and identify genes that are affected by overexpression of Kctd15. Among many genes identified we chose Duf domain containing protein 1(ddcp1), Platelet-Derived Growth Factor Receptor a (pdgfra), Complement factor properdin (cfp), Zinc Finger SWIM-Type Containing 5 (zswim5), and complement component 3 (C3) to examine their expression by whole mount in situ hybridization. Our work points to a possible role for Kctd15 in the regulation of NC formation and other steps in embryonic development.

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“…The ETS family of TFs are known for their role in regulating a spectrum of developmental processes. For example, Etv1 is involved in differentiation of dopaminergic neurons (Flames and Hobert, 2009) and cerebellum granule cells (Abe et al, 2011), while Ets1 plays a role in neural crest differentiation (Wang et al, 2015). …”

Section: Resultsmentioning

confidence: 99%

Epigenomic Landscapes of hESC-Derived Neural Rosettes: Modeling Neural Tube Formation and Diseases

et al. 2017

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SUMMARY We currently lack a comprehensive understanding of the mechanisms underlying neural tube formation and their contributions to neural tube defects (NTDs). Developing a model to study such a complex morphogenetic process is critical, especially one that models human-specific aspects. Three-dimensional, hESCs-derived neural rosettes (NRs) provide a powerful resource for in vitro modeling of human neural tube formation. Epigenomic maps reveal enhancer elements unique to NRs relative to 2D systems. A master regulatory network illustrates that key NR properties are related to their epigenomic landscapes. We found that folate-associated DNA methylation changes were enriched within NR regulatory elements near genes involved in neural tube formation and metabolism. Our comprehensive regulatory maps offer insights into the mechanisms by which folate may prevent NTDs. Lastly, our distal regulatory maps provide a better understanding of the potential role of neurological disorder-associated SNPs.

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The Proto-oncogene Transcription Factor Ets1 Regulates Neural Crest Development through Histone Deacetylase 1 to Mediate Output of Bone Morphogenetic Protein Signaling

Cited by 35 publications

References 59 publications

Histone deacetylase activity has an essential role in establishing and maintaining the vertebrate neural crest

Histone deacetylase activity has an essential role in establishing and maintaining the vertebrate neural crest

Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest

Epigenomic Landscapes of hESC-Derived Neural Rosettes: Modeling Neural Tube Formation and Diseases

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