The prothymosin α gene is specifically expressed in ectodermal and mesodermal regions during early postimplantation mouse embryogenesis

Amo, Francisco Franco del; Freire, Manuel

doi:10.1016/0014-5793(94)01427-3

Cited by 6 publications

References 32 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Differential expression of duplicated genes for prothymosin alpha during zebrafish development

Donizetti

Liccardo

Esposito

et al. 2008

Developmental Dynamics

View full text Add to dashboard Cite

We show that ptma, a single copy gene found in all organisms investigated so far, is duplicated in zebrafish. The two genes, ptmaa and ptmab, are individually controlled as indicated by their different expression patterns during embryonic development. Only the ptmab transcript is observed at 4 and 8 hpf of development in all embryonic cells, whereas both genes are expressed at later stages as revealed by in situ hybridization studies. In most cases, the two genes are expressed in the same territories, but only the ptmaa transcript was found in the trigeminal ganglion and in endodermal pouches. In the eye, at 72 hpf, the ptmaa and ptmab transcripts were found in amacrine cells, whereas only the ptmab transcript appeared in horizontal cells. The existence of two prothymosin genes indicates that their function in cell proliferation and differentiation is more complex in fishes than in mammals.

show abstract

Differential expression of duplicated genes for prothymosin alpha during zebrafish development

Donizetti

Liccardo

Esposito

et al. 2008

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

Transcriptional profiling using a novel cDNA array identifies differential gene expression during porcine embryo elongation

et al. 2005

View full text Add to dashboard Cite

A novel porcine cDNA array, containing 1,015 PCR products selected for embryonic expression, was used for transcriptional profiling of conceptuses at four stages of peri-implantation development. Total conceptus RNA from small spherical, large spherical, tubular, and filamentous stages was amplified, converted to cDNA, and hybridized to membranes. Initially, normalized signal intensities obtained using cDNA from total RNA or from amplified RNA were compared. Uniform distribution of P-values associated with t-tests conducted for each gene indicated no evidence that amplification introduced bias. Analysis of data obtained by using amplified targets and the novel array identified genes differentially expressed across stages. Such genes were identified by testing for significant stage effects in gene-specific mixed models. A total of nine genes were declared differentially expressed. Six of the nine genes had P-values less than 0.001, and a false discovery rate of approximately 17% was associated with this significance threshold. Two out of six genes were significant when using the Bonferroni method to control the probability of one or more false positives. The other three genes had P-values between 0.001 and 0.01 and exhibited differences greater than twofold between stages. All four genes selected for confirmation (steroidogenic acute regulatory protein, interleukin 1 beta, transforming growth factor beta 3, and thymosin beta 10) were shown to be differentially expressed by using quantitative real time RT-PCR. Our study shows that RNA amplification is useful for transcriptional profiling with limiting porcine embryonic RNA, and that this novel targeted array can detect differential gene expression during trophoblastic elongation. Finally, our results contribute to an increased understanding of the temporal patterns of expression of known genes controlling conceptus development, as well as identify novel genes also differentially regulated during implantation.

show abstract

Nuclear Distribution of Prothymosin α and Parathymosin: Evidence That Prothymosin α Is Associated with RNA Synthesis Processing and Parathymosin with Early DNA Replication

Vareli

Frangou-Lazaridis

Kraan

et al. 2000

Experimental Cell Research

View full text Add to dashboard Cite

The prothymosin α gene is specifically expressed in ectodermal and mesodermal regions during early postimplantation mouse embryogenesis

Cited by 6 publications

References 32 publications

Differential expression of duplicated genes for prothymosin alpha during zebrafish development

Differential expression of duplicated genes for prothymosin alpha during zebrafish development

Transcriptional profiling using a novel cDNA array identifies differential gene expression during porcine embryo elongation

Nuclear Distribution of Prothymosin α and Parathymosin: Evidence That Prothymosin α Is Associated with RNA Synthesis Processing and Parathymosin with Early DNA Replication

Contact Info

Product

Resources

About