The proteomics contribution to the counter-bioterrorism toolbox in the post-COVID-19 era

Armengaud, Jean

doi:10.1080/14789450.2020.1822745

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…N-Protein variants such as those deciphered in the present study might be important for such developments. While the vaccine evading problem may be a Black Swan theoretical idea [22], understanding how SARS-CoV-2 evolves and anticipating the response to these novel variants remain of utmost importance [23]. A very recent study based on whole genome SARS-CoV-2 deep sequencing of numerous clinical samples reported low levels of within-host diversity, but a dynamic landscape during infection [24].…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of SARS-CoV-2 virus produced in cell culture revealed by shotgun proteomics and supported by genome sequencing

et al. 2021

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COVID-19 is the most disturbing pandemic of the past hundred years. Its causative agent, the SARS-CoV-2 virus, has been the subject of an unprecedented investigation to characterize its molecular structure and intimate functioning. While markers for its detection have been proposed and several diagnostic methodologies developed, its propensity to evolve and evade diagnostic tools and the immune response is of great concern. The recent spread of new variants with increased infectivity requires even more attention. Here, we document how shotgun proteomics can be useful for rapidly monitoring the evolution of the SARS-CoV-2 virus. We evaluated the heterogeneity of purified SARS-CoV-2 virus obtained after culturing in the Vero E6 cell line. We found that cell culture induces significant changes that are translated at the protein level, such changes being detectable by tandem mass spectrometry. Production of viral particles requires careful quality control which can be easily performed by shotgun proteomics. Although considered relatively stable so far, the SARS-CoV-2 genome turns out to be prone to frequent variations. Therefore, the sequencing of SARS-CoV-2 variants from patients reporting only the consensus genome after its amplification would deserve more attention and could benefit from more in-depth analysis of low level but crystal-clear signals, as well as complementary and rapid analysis by shotgun proteomics. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Heterogeneity of SARS-CoV-2 virus produced in cell culture revealed by shotgun proteomics and supported by genome sequencing

et al. 2021

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“…Given the mass impact of SARS-CoV-2 on health and everyday life during 2020, the demand for rapid detection, therapeutics, and treatment has been vocalized from all members of society including scientists, politicians, and the general public. As vaccines for COVID-19 are administered across the globe, we must continue to prioritize techniques and methods that rapidly, efficiently, and cost-effectively identify and characterize pathogens so appropriate responses and treatments that minimize disease severity and duration can be developed [2].…”

Section: Virus Outbreaks Are Frequent Eventsmentioning

confidence: 99%

How lipidomics can transform our understanding of virus infections

Kyle

2021

Expert Review of Proteomics

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“…Unprecedented efforts were rapidly implemented by the scientific community to better characterize the infectious mechanisms of SARS-CoV-2, to understand the disease and its consequences, propose new antiviral solutions, and more importantly to develop vaccines. [3] A collective mass spectrometry effort was launched to study COVID-19 and propose alternative solutions for the detection of SARS-CoV-2. In their letter of intent, [4] this coalition of nearly 600 primed scientists from sixty countries anticipated that proteomics could play a key role in understanding COVID-19.…”

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confidence: 99%

Proteomics in the COVID‐19 Battlefield: First Semester Check‐Up

Grenga

Armengaud

2020

Proteomics

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Proteomics offers a wide collection of methodologies to study biological systems at the finest granularity. Faced with COVID‐19, the most worrying pandemic in a century, proteomics researchers have made significant progress in understanding how the causative virus hijacks the host's cellular machinery and multiplies exponentially, how the disease can be diagnosed, and how it develops, as well as its severity predicted. Numerous cellular targets of potential interest for the development of new antiviral drugs have been documented. Here, the most striking results obtained in the proteomics field over this first semester of the pandemic are presented. The molecular machinery of SARS‐CoV‐2 is much more complex than initially believed, as many post‐translational modifications can occur, leading to a myriad of proteoforms and a broad heterogeneity of viral particles. The interplay of protein–protein interactions, protein abundances, and post‐translational modifications has yet to be fully documented to provide a full picture of this intriguing but lethal biological threat. Proteomics has the potential to provide rapid detection of the SARS‐CoV‐2 virus by mass spectrometry proteotyping, and to further increase the knowledge of severe respiratory syndrome COVID‐19 and its long‐term health consequences.

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The proteomics contribution to the counter-bioterrorism toolbox in the post-COVID-19 era

Cited by 5 publications

References 27 publications

Heterogeneity of SARS-CoV-2 virus produced in cell culture revealed by shotgun proteomics and supported by genome sequencing

Heterogeneity of SARS-CoV-2 virus produced in cell culture revealed by shotgun proteomics and supported by genome sequencing

How lipidomics can transform our understanding of virus infections

Proteomics in the COVID‐19 Battlefield: First Semester Check‐Up

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