The proteomic profile of whole and glandular saliva in healthy pain-free subjects

Jasim, Hajer; Olausson, Patrik; Hedenberg-Magnusson, Britt; Ernberg, Malin; Ghafouri, Bijar

doi:10.1038/srep39073

Cited by 70 publications

(70 citation statements)

References 43 publications

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“…Finally, as part of the sample collection protocol of this survey study, stimulated saliva samples were collected. The protein concentration was higher in the unstimulated saliva samples than the stimulated saliva samples, however, the unstimulated saliva samples possessed greater inter-and intraindividual variation than the stimulated saliva samples [23].…”

Section: Discussionmentioning

confidence: 71%

Salivary Cytokine Biomarker Concentrations in Relation to Obesity and Periodontitis

Syrjalainen

Gürsoy

et al. 2019

JCM

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Systemic low-grade inflammation is associated with obesity. Our aim was to examine the association between obesity and salivary biomarkers of periodontitis. Salivary interleukin (IL)-1-receptor antagonist (IL-1Ra), IL-6, IL-8, IL-10, and tumor necrosis factor (TNF)-α concentrations were measured from 287 non-diabetic obese (body mass index (BMI) of >35 kg/m2) individuals and 293 normal-weight (BMI of 18.5–25 kg/m2) controls. Periodontal status was defined according to a diagnostic cumulative risk score (CRS) to calculate the risk of having periodontitis (CRS I, low risk; CRS II, medium risk; CRS III, high risk). In the whole population, and especially in smokers, higher IL-8 and lower IL-10 concentrations were detected in the obese group compared to the control group, while in non-smoking participants, the obese and control groups did not differ. IL-1Ra and IL-8 concentrations were higher in those with medium or high risk (CRS II and CRS III, p < 0.001) of periodontitis, whereas IL-10 and TNF-α concentrations were lower when compared to those with low risk (CRS I). In multivariate models adjusted for periodontal status, obesity did not associate with any salivary cytokine concentration. In conclusion, salivary cytokine biomarkers are not independently associated with obesity and concentrations are dependent on periodontal status.

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Section: Discussionmentioning

confidence: 71%

Salivary Cytokine Biomarker Concentrations in Relation to Obesity and Periodontitis

Syrjalainen

Gürsoy

et al. 2019

JCM

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“…Stimulated whole saliva was collected in the morning using a standardized protocol, as described previously [22,23]. In order to prevent any contamination from other sources, the participants were asked to rinse their mouth with water before saliva collection.…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

“…Saliva is an outstanding body fluid containing a complex mixture of proteins, peptides, and other substances that may yield information about the pathophysiology of TMD myalgia and can be used to identify new candidate proteins of the disorder. Proteomic analysis of saliva from patients with TMD myalgia represents a new potential field of research, as the proteomic techniques are constantly improving [22][23][24]. Two studies, so far, have investigated the salivary proteome of patients with widespread myalgia.…”

Section: Introductionmentioning

confidence: 99%

Protein Signature in Saliva of Temporomandibular Disorders Myalgia

Jasim

Ernberg

Carlsson

et al. 2020

IJMS

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In the last years, several attempts have been made to study specific biological markers of temporomandibular disorders (TMD). So far, no laboratory tests have been appropriately validated for the diagnosis and prognosis of these disorders. This study aimed to investigate the proteomic profile of the whole stimulated saliva of TMD myalgia patients in order to evaluate potential diagnostic and/or prognostic salivary candidate proteins which could be useful for the management of TMD. Twenty patients diagnosed with TMD myalgia according to the validated Diagnostic Criteria for TMD (DC/TMD) and 20 matched healthy pain-free controls were enrolled. Saliva samples were collected in the morning. Comparative proteomic analysis was performed with two-dimensional gel electrophoresis followed by identification with liquid chromatography-tandem mass spectrometry. Statistical analysis of the quantitative proteomics data revealed that 20 proteins were significantly altered in patients compared to controls. Among these proteins, 12 showed significantly increased levels, and 8 showed significantly decreased levels in patients with TMD myalgia compared to controls. The identified proteins are involved in metabolic processes, immune response, and stress response. This proteomic study shows that the salivary protein profile can discriminate patients with TMD myalgia from healthy subjects, but the protein signature has no correlation with the clinical features of TMD myalgia. Additional studies are needed to validate our observations in additional sample sets and to continue assessing the utility of saliva as a suitable sample for studying processes related to TMD myalgia.

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“…Whole saliva encompasses an amalgamation of fluids from multiple secretory glands within the oral cavity . Although it contains many peptides found in blood, saliva has a much smaller protein dynamic range than serum or plasma, which makes it advantageous for interrogating low‐abundant proteins . Additionally, it can be collected in a simple and pain‐free manner, which is ideal for serial disease monitoring.…”

Section: Salivamentioning

confidence: 99%

Tumor‐Derived Extracellular Vesicles as a Novel Source of Protein Biomarkers for Cancer Diagnosis and Monitoring

Ruhen

Meehan

2019

Proteomics

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“Liquid biopsies” have received attention as a complementary tool for traditional tissue biopsies that may enhance the spectrum of analysis for tumor‐derived factors. One such factor gaining prominence in the liquid biopsy field is extracellular vesicles (EVs), membrane‐bound nanovesicles which are secreted by cells into biofluids such as blood, urine, and saliva. EVs are released in both physiological and pathological conditions and can transport a variety of molecules, including proteins, metabolites, RNA, microRNAs, and DNA, to distant sites throughout the body. As such, they are emerging as a promising source of tumor biomarkers for the noninvasive diagnosis, prognosis, and monitoring of cancer patients. In particular, the wealth of tumor‐related information that can be gleaned from the EV proteomic cargo has become apparent through mass spectrometric analysis, which has provided new benchmarks for clinically focused biomarker research. In this review, the current achievements in the use of MS for identifying potential EV‐derived protein biomarkers of cancer are explored, and the techniques and challenges involved in this pursuit are summarized.

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The proteomic profile of whole and glandular saliva in healthy pain-free subjects

Cited by 70 publications

References 43 publications

Salivary Cytokine Biomarker Concentrations in Relation to Obesity and Periodontitis

Salivary Cytokine Biomarker Concentrations in Relation to Obesity and Periodontitis

Protein Signature in Saliva of Temporomandibular Disorders Myalgia

Tumor‐Derived Extracellular Vesicles as a Novel Source of Protein Biomarkers for Cancer Diagnosis and Monitoring

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