The proteome of Chlamydomonas reinhardtii during phosphorus depletion and repletion

Plouviez, Maxence; Abyadeh, Morteza; Hasan, Mafruha T.; Mirzaei, Mehdi; Paulo, João A.; Guieysse, Benoît

doi:10.1016/j.algal.2023.103037

Cited by 15 publications

(28 citation statements)

References 62 publications

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“…In Chlamydomonas reinhardtii , the supply of dissolved P in the media has been documented to influence luxury uptake of P, while light supply does not (Plouviez et al., 2023). Our data however suggest an association between luxury P accumulation and growth rate, rather than dissolved P or light supply.…”

Section: Resultsmentioning

confidence: 99%

Elemental and macromolecular plasticity of Chlamydomonas reinhardtii (Chlorophyta) in response to resource limitation and growth rate

Isanta‐Navarro,

Peoples,

Bras

et al. 2024

Journal of Phycology

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With the ongoing differential disruption of the biogeochemical cycles of major elements that are essential for all life (carbon, nitrogen, and phosphorus), organisms are increasingly faced with a heterogenous supply of these elements in nature. Given that photosynthetic primary producers form the base of aquatic food webs, impacts of changed elemental supply on these organisms are particularly important. One way that phytoplankton cope with the differential availability of nutrients is through physiological changes, resulting in plasticity in macromolecular and elemental biomass composition. Here, we assessed how the green alga Chlamydomonas reinhardtii adjusts its macromolecular (e.g., carbohydrates, lipids, and proteins) and elemental (C, N, and P) biomass pools in response to changes in growth rate and the modification of resources (nutrients and light). We observed that Chlamydomonas exhibits considerable plasticity in elemental composition (e.g., molar ratios ranging from 124 to 971 for C:P, 4.5 to 25.9 for C:N, and 15.1 to 61.2 for N:P) under all tested conditions, pointing to the adaptive potential of Chlamydomonas in a changing environment. Exposure to low light modified the elemental and macromolecular composition of cells differently than limitation by nutrients. These observed differences, with potential consequences for higher trophic levels, included smaller cells, shifts in C:N and C:P ratios (due to proportionally greater N and P contents), and differential allocation of C among macromolecular pools (proportionally more lipids than carbohydrates) with different energetic value. However, substantial pools of N and P remained unaccounted for, especially at fast growth, indicating accumulation of N and P in forms we did not measure.

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Section: Resultsmentioning

confidence: 99%

Elemental and macromolecular plasticity of Chlamydomonas reinhardtii (Chlorophyta) in response to resource limitation and growth rate

Isanta‐Navarro,

Peoples,

Bras

et al. 2024

Journal of Phycology

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“…Complete nutrients were required to restore RNA levels and to resume growth. Some studies have reported that a longer Pi deprivation period is required for maximum Pi overplus response (34, 36) but when time is used as the variable it is difficult to compare experiments performed under different experimental conditions. A significant contribution of this study is to demonstrate that lowest in cell polyP corresponds to the point of growth cessation and is optimal time for Pi repletion to maximise the Pi overplus response.…”

Section: Discussionmentioning

confidence: 99%

“…Depriving cells of Pi until lowest polyP / growth cessation followed by resupply of Pi with complete nutrients led to resumed growth and complete removal of media Pi within 12 h ( Figure 6A ) much more rapid than without prior Pi deprivation ( Figure 2C ) or with longer Pi deprivation ( Figure 6B ). While the effect of Pi deprivation on both Pi overplus and Pi uptake in Chlamydomonas has been studied previously and the correlation between increased biomass and Pi uptake noted (34), the role of other nutrients has not been explored despite clear evidence that the responses to Pi, sulphate and nitrogen are interrelated (1, 2, 14, 41, 42). We used PCA to link the correlation of nutrient uptake rates to the initial nutrient concentration of PO 4 -P, NH 4 -N and SO 4 -S according to both Pi deprivation periods and Pi repletion types tested ( Figure 7 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, the performance of microalgae in this regard is unpredictable due to the lack of solid underpinning knowledge regarding the physiological responses of microalgae and the allocation of Pi between different pools under conditions of nutrient deprivation and resupply. Most published studies carry out nutrient deprivation for a specified time, without measuring any specific physiological parameter (7,(31)(32)(33)(34). This makes comparing the results practically impossible.…”

Section: Introductionmentioning

confidence: 99%

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Phosphate overplus response inChlamydomonas reinhardtii: polyphosphate dynamics to monitor phosphate uptake and turnover

Zúñiga-Burgos,

Saiardi,

Camargo-Valero

et al. 2024

Preprint

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Many micro-organisms store inorganic phosphate (Pi) in the form of polyphosphate (polyP) and exhibit in-cell polyP accumulation, a phenomenon known as ‘phosphate overplus response’, when resupplied with Pi after a period of deprivation. Quantitative and qualitative methods were used to follow the dynamics of polyP synthesis and turnover in four strains of Chlamydomonas reinhardtii during Pi deprivation followed by nutrient resupply. The lowest level of in-cell polyP during Pi deprivation, which also correlates with the cessation of growth, is the key parameter for the timing of Pi resupply to maximise the Pi overplus response Additional nutrients do not affect the size of the overplus response, but they are important for continued growth and maximal Pi removal from the media. Tracking polyP allows the correct time for nutrient resupply to be determined and therefore a reproducible Pi overplus response to be achieved. Depending on whether maximum cellular phosphorus (P) content or maximum Pi removal is desired different strategies may be required – e,g., Pi deprivation until growth cessation then resupplying complete nutrients gives the best trade-off between high in-cell P accumulation, high Pi uptake and algal biomass growth. Although polyP levels are maintained after Pi resupply, the polymer is dynamically remodelled. IP6increases during this time. This increase does not precede polyP synthesis as predicted by a model where inositol phosphates switch on polyP synthesis. One strain tested, CC-5325, shows enhanced Pi uptake and levels of polyP and total in-cell P, suggesting that strain selection is also important.

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“…Indeed, moderate correlations of ~0.4 have been widely reported between the abundance of proteins and their corresponding mRNAs in both prokaryotes and eukaryotes [ 34 , 39 ]. Conversely, Plouviez et al [ 40 ] recently showed, by temporal analysis of both datasets, that the transcriptome and the proteome related to phosphorus metabolism are synchronized in Chlamydomonas . This provided insight into the turnover and regulation of Vacuolar Transport Chaperone proteins, key proteins involved in polyphosphate synthesis in microalgae [ 40 , 41 , 42 ].…”

Section: Proteomicsmentioning

confidence: 99%

Key Proteomics Tools for Fundamental and Applied Microalgal Research

Plouviez,

Dubreucq

2024

Proteomes

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Microscopic, photosynthetic prokaryotes and eukaryotes, collectively referred to as microalgae, are widely studied to improve our understanding of key metabolic pathways (e.g., photosynthesis) and for the development of biotechnological applications. Omics technologies, which are now common tools in biological research, have been shown to be critical in microalgal research. In the past decade, significant technological advancements have allowed omics technologies to become more affordable and efficient, with huge datasets being generated. In particular, where studies focused on a single or few proteins decades ago, it is now possible to study the whole proteome of a microalgae. The development of mass spectrometry-based methods has provided this leap forward with the high-throughput identification and quantification of proteins. This review specifically provides an overview of the use of proteomics in fundamental (e.g., photosynthesis) and applied (e.g., lipid production for biofuel) microalgal research, and presents future research directions in this field.

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The proteome of Chlamydomonas reinhardtii during phosphorus depletion and repletion

Cited by 15 publications

References 62 publications

Elemental and macromolecular plasticity of Chlamydomonas reinhardtii (Chlorophyta) in response to resource limitation and growth rate

Elemental and macromolecular plasticity of Chlamydomonas reinhardtii (Chlorophyta) in response to resource limitation and growth rate

Phosphate overplus response inChlamydomonas reinhardtii: polyphosphate dynamics to monitor phosphate uptake and turnover

Key Proteomics Tools for Fundamental and Applied Microalgal Research

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