2009
DOI: 10.1002/pmic.200700476
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The proteome of Salmonella Typhimurium grown under in vivo‐mimicking conditions

Abstract: To successfully infect a host, it is a prerequisite for enteric pathogens such as Salmonella enterica serovar Typhimurium to adapt to their environment, in casu the gastrointestinal tract. The adoption of an appropriate lifestyle is triggered by environmental signals such as the low oxygen availability and high osmolarity prevalent in the gut. In order to gain more insight in the changes that are induced when S. Typhimurium is adapting to these particular conditions, we used 2-D DIGE technology to investigate … Show more

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Cited by 44 publications
(31 citation statements)
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“…Recent results from Craig and coworkers (25) highlight the importance of aerobic respiration for host colonization; this would explain why a strictly anaerobic process such as the FHL/H 2 evolution is not important in vivo. FHL and associated H 2 production could be useful in other environments or within other hosts, as the metabolic flexibility of Salmonella is becoming more appreciated (37)(38)(39)(40). Our results suggest that evolution of H 2 by the activity of Hyc is not a requirement for either the virulence or the gut survival or fecal transmission of S. Typhimurium.…”
Section: Discussionmentioning
confidence: 57%
“…Recent results from Craig and coworkers (25) highlight the importance of aerobic respiration for host colonization; this would explain why a strictly anaerobic process such as the FHL/H 2 evolution is not important in vivo. FHL and associated H 2 production could be useful in other environments or within other hosts, as the metabolic flexibility of Salmonella is becoming more appreciated (37)(38)(39)(40). Our results suggest that evolution of H 2 by the activity of Hyc is not a requirement for either the virulence or the gut survival or fecal transmission of S. Typhimurium.…”
Section: Discussionmentioning
confidence: 57%
“…2D-PAGE was first introduced in 1975(O'Farrell, 1975, and typically allows the detection of only a few hundred proteins after their separation, thereby covering only 'low-complexity' proteomes. With recent application of various fluorescent dyes in this approach, such as 2D fluorescence difference gel electrophoresis (2D DIGE), the coverage and sensitivity of gel-based protein analysis have been significantly improved (Sonck et al, 2009); however, it is still not compatible with gel-free proteomics techniques that can detect several thousand proteins of large dynamic ranges in a single run and that can also provide better detection for low-abundance proteins and insoluble membrane-spanning proteins (Wolff et al, 2006). Even with some improvement, currently neither the 2D-PAGE nor the LC-MS/MS approach is close to saturating identifications in even small bacterial proteomes: typically only 20-40 % of the proteome can be detected, and these are identification approaches and do not provide quantitative information.…”
Section: Proteomicsmentioning
confidence: 99%
“…The ADI pathway of S. Typhimurium appears to be functional, because the expression of the putative ADI cluster increased OTC activity (43). In the present study, we investigated the role of the ADI system in S. Typhimurium pathogenesis.…”
mentioning
confidence: 99%