The Proteome and Lipidome of Synechocystis sp. PCC 6803 Cells Grown under Light-Activated Heterotrophic Conditions*

Plohnke, Nicole; Seidel, Tobias; Kahmann, Uwe; Rögner, Matthias; Schneider, Dirk; Rexroth, Sascha

doi:10.1074/mcp.m114.042382

Cited by 36 publications

(46 citation statements)

References 80 publications

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“…3A). This is consistent with results obtained in (Plohnke et al 2015). The RO mutant exhibited similar lipid composition as the WT, but with lower portion of 34:3.…”

Section: Resultssupporting

confidence: 94%

“…In agreement with earlier results (Plohnke et al 2015), in WT cells 34:2 and 34:1 were the most abundant PG species (Fig. 3D).…”

Section: Resultssupporting

confidence: 93%

“…In the WT cells grown at normal temperature the mol percentage distribution of the four main lipid classes were similar to earlier published results (Plohnke et al 2015). Although in the WT and mutant lines MGDG was the most abundant lipid class, there were noticeable differences in the distribution of this molecule in the Car and lipid mutants, as well as in the cells grown at different temperatures (Fig.…”

Section: Resultssupporting

confidence: 91%

“…A comparison of the saturation levels in the different lipid classes reveals substantial differences in the ways how lipid content was remodelled in the xanthophyll and polyunsaturated lipid mutants. In the WT cells unsaturation level of SQDG and PG dropped by about 80% and 40%, respectively, compared to those of MGDG and DGDG, in agreement with previous reports (Murata et al 1992; Plohnke et al 2015). By contrast, in the RO mutant the unsaturation level of SQDG is about twice higher than that of the WT, and in the AD mutant PG unsaturation remained unchanged.…”

Section: Discussionsupporting

confidence: 93%

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Lipid and carotenoid cooperation-driven adaptation to light and temperature stress in Synechocystis sp. PCC6803

Zakar

Herman

Vajravel

et al. 2017

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Polyunsaturated lipids are important components of photosynthetic membranes. Xanthophylls are the main photoprotective agents, can assist in protection against light stress, and are crucial in the recovery from photoinhibition. We generated the xanthophyll- and polyunsaturated lipid-deficient ROAD mutant of Synechocystis sp. PCC6803 (Synechocystis) in order to study the little-known cooperative effects of lipids and carotenoids (Cars). Electron microscopic investigations confirmed that in the absence of xanthophylls the S-layer of the cellular envelope is missing. In wild-type (WT) cells, as well as the xanthophyll-less (RO), polyunsaturated lipid-less (AD), and the newly constructed ROAD mutants the lipid and Car compositions were determined by MS and HPLC, respectively. We found that, relative to the WT, the lipid composition of the mutants was remodeled and the Car content changed accordingly. In the mutants the ratio of non-bilayer-forming (NBL) to bilayer-forming (BL) lipids were found considerably lower. Xanthophyll to β-carotene ratio increased in the AD mutant. In vitro and in vivo methods demonstrated that saturated, monounsaturated lipids and xanthophylls may stabilize the trimerization of Photosystem I (PSI). Fluorescence induction and oxygen-evolving activity measurements revealed increased light sensitivity of RO cells compared to those of the WT. ROAD showed a robust increase in light susceptibility and reduced recovery capability, especially at moderate low (ML) and moderate high (MH) temperatures, indicating a cooperative effect of xanthophylls and polyunsaturated lipids. We suggest that both lipid unsaturation and xanthophylls are required for providing the proper structure and functioning of the membrane environment that protects against light and temperature stress.

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“…3A). This is consistent with results obtained in (Plohnke et al 2015). The RO mutant exhibited similar lipid composition as the WT, but with lower portion of 34:3.…”

Section: Resultssupporting

confidence: 94%

“…In agreement with earlier results (Plohnke et al 2015), in WT cells 34:2 and 34:1 were the most abundant PG species (Fig. 3D).…”

Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 91%

Section: Discussionsupporting

confidence: 93%

See 2 more Smart Citations

Lipid and carotenoid cooperation-driven adaptation to light and temperature stress in Synechocystis sp. PCC6803

Zakar

Herman

Vajravel

et al. 2017

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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show abstract

“…With the development of high-accuracy mass spectrometry and the steadily progressing sequencing power of nanoLC-MS/MS systems, deeper analysis of proteomes and their post-translational modifications became available (39). Today, gel-free sample preparations together with stable isotope labeling strategies set the standard for studying dynamic responses of the proteome and its post-translational modifications on global levels.…”

Section: Discussion Dimethylation Labeling Enables Deep Analysis Of (mentioning

confidence: 99%

Chlorosis as a Developmental Program in Cyanobacteria: The Proteomic Fundament for Survival and Awakening

Spät

Klotz

Rexroth

et al. 2018

Molecular & Cellular Proteomics

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Thylakoid Development and Galactolipid Synthesis in Cyanobacteria

Awai

2016

Subcellular Biochemistry

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Cyanobacteria carry out oxygenic photosynthesis and share many features with chloroplasts, including thylakoid membranes, which are mainly composed of membrane lipids and protein complexes that mediate photosynthetic electron transport. Although the functions of the various thylakoid protein complexes have been well characterized, the details underlying the biogenesis of thylakoid membranes remain unclear. Galactolipids are the major constituents of the thylakoid membrane system, and all the genes involved in galactolipid biosynthesis were recently identified. In this chapter, I summarize recent advances in our understanding of the factors involved in thylakoid development, including regulatory proteins and enzymes that mediate lipid biosynthesis.

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The Proteome and Lipidome of Synechocystis sp. PCC 6803 Cells Grown under Light-Activated Heterotrophic Conditions*

Cited by 36 publications

References 80 publications

Lipid and carotenoid cooperation-driven adaptation to light and temperature stress in Synechocystis sp. PCC6803

Lipid and carotenoid cooperation-driven adaptation to light and temperature stress in Synechocystis sp. PCC6803

Chlorosis as a Developmental Program in Cyanobacteria: The Proteomic Fundament for Survival and Awakening

Thylakoid Development and Galactolipid Synthesis in Cyanobacteria

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