The proteins (12 and 15 kDa) isolated from heat‐killed <i>Lactobacillus plantarum</i> L67 induces apoptosis in HT‐29 cells

Song, Sooyeon; Oh, Sejong; Lim, Kye-Taek

doi:10.1002/cbf.3094

“…Surface receptors for extrinsic apoptosis, such as TNF-α, are produced by immune system cells and Fas, which are able to activate the cytosolic protease, caspase-8. Caspase-8 activates caspase-3, caspase-6, and caspase-7 that leading to apoptosis induction (32). In this way, Kim et al revealed that B. adolescentis potentiated the production of TNF-α, as a cytokine that induces apoptosis, and their results supported our results (31).…”

Section: Discussionsupporting

confidence: 88%

Anticancer Effects of Bifidobacteria on Colon Cancer Cell Lines

Faghfoori

¹

,

Faghfoori

²

,

Saber

³

et al. 2020

Preprint

0

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Background: Colorectal cancer (CRC), with a growing incidence trend worldwide, is resistant to apoptosis and have the uncontrolled proliferation. It is recently reported that probiotic microorganisms exert anticancer effects. The genus Bifidobacterium, one of the dominant bacterial populations in the gastrointestinal tract, has received increasing attention because of widespread interest in using as health-promoting microorganisms. Therefore, the present study aimed to assess the apoptotic effects of some bifidobacteria species on colon cancer cell lines.Methods: The cytotoxicity evaluations performed using MTT assay and FACS-flow cytometry tests. Also, the effects of five species of bifidobacteria secretion metabolites on the expression level of anti- or pro-apoptotic genes including BAD, Bcl-2, Caspase-3, Caspase-8, Caspase-9, and Fas-R studied by real-time polymerase chain reaction (RT-PCR) method. Results: The cell-free supernatant of all studied bifidobacteria significantly decreased the survival rates of colon cancer cells compared with control groups. Flow cytometric and RT-PCR results indicated that apoptosis is induced by bifidobacteria secretion metabolites and the mechanism for the action of bifidobacteria species in CRC prevention could be down-regulation and up-regulation of anti-apoptotic and, pro-apoptotic genes.Conclusions: In the present study, different bifidobacteria species showed anticancer activity on colorectal cancer cells through down-regulation and up-regulation of anti-apoptotic and pro-apoptotic genes. However, further studies are required to clarify the exact mechanism of apoptosis induction by bifidobacteria species.

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“…Surface receptors for extrinsic apoptosis, such as TNF-α, are produced by immune system cells and Fas, which are able to activate the cytosolic protease and caspase-8. Then, the caspase-8 activates caspase-3, caspase-6, and caspase-7 that leading to extrinsic apoptosis induction (38). In this way, Kim et al revealed that B. adolescentis potentiated the production of TNF-α, as a cytokine that induces apoptosis, and their results supported our results about the induction of apoptosis by this bacteria species (36).…”

Section: Discussionsupporting

confidence: 86%

Anticancer effects of Bifidobacteria on colon cancer cell lines

Faghfoori

¹

,

Faghfoori

²

,

Saber

³

et al. 2021

Preprint

0

View full text Add to dashboard Cite

Background: Colorectal cancer (CRC), with a growing incidence trend worldwide, is resistant to apoptosis and have the uncontrolled proliferation. It is recently reported that probiotic microorganisms exert anticancer effects. The genus Bifidobacterium, one of the dominant bacterial populations in the gastrointestinal tract, has received increasing attention because of widespread interest in using as health-promoting microorganisms. Therefore, the present study aimed to assess the apoptotic effects of some bifidobacteria species on colon cancer cell lines.Methods: The cytotoxicity evaluations performed using MTT assay and FACS-flow cytometry tests. Also, the effects of five species of bifidobacteria secretion metabolites on the expression level of anti- or pro-apoptotic genes including BAD, Bcl-2, Caspase-3, Caspase-8, Caspase-9, and Fas-R studied by real-time polymerase chain reaction (RT-PCR) method. Results: The cell-free supernatant of all studied bifidobacteria significantly decreased the survival rates of colon cancer cells compared with control groups. Flow cytometric and RT-PCR results indicated that apoptosis is induced by bifidobacteria secretion metabolites and the mechanism for the action of bifidobacteria species in CRC prevention could be down-regulation and up-regulation of anti-apoptotic and, pro-apoptotic genes.Conclusions: In the present study, different bifidobacteria species showed anticancer activity on colorectal cancer cells through down-regulation and up-regulation of anti-apoptotic and pro-apoptotic genes. However, further studies are required to clarify the exact mechanism of apoptosis induction by bifidobacteria species.

show abstract

“…Surface receptors for extrinsic apoptosis, such as TNF-α, are produced by immune system cells and Fas, which are able to activate the cytosolic protease and caspase-8. Then, the caspase-8 activates caspase-3, caspase-6, and caspase-7 that leading to extrinsic apoptosis induction [ 38 ]. In this way, Kim et al revealed that B. adolescentis enhances the production of TNF-α, as a cytokine that induces apoptosis, and their results supported our findings about the induction of apoptosis by this bacteria species[ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Anticancer effects of bifidobacteria on colon cancer cell lines

Faghfoori

¹

,

Faghfoori

²

,

Saber

³

et al. 2021

View full text Add to dashboard Cite

Background Colorectal cancer (CRC), with a growing incidence trend worldwide, is resistant to apoptosis and has uncontrolled proliferation. It is recently reported that probiotic microorganisms exert anticancer effects. The genus Bifidobacterium, one of the dominant bacterial populations in the gastrointestinal tract, has received increasing attention because of widespread interest in using it as health-promoting microorganisms. Therefore, the present study aimed to assess the apoptotic effects of some bifidobacteria species on colon cancer cell lines. Methods The cytotoxicity evaluations performed using MTT assay and FACS-flow cytometry tests. Also, the effects of five species of bifidobacteria secretion metabolites on the expression level of anti- or pro-apoptotic genes including BAD, Bcl-2, Caspase-3, Caspase-8, Caspase-9, and Fas-R studied by real-time polymerase chain reaction (RT-PCR) method. Results The cell-free supernatant of all studied bifidobacteria significantly decreased the survival rates of colon cancer cells compared with control groups. Flow cytometric and RT-PCR results indicated that apoptosis is induced by bifidobacteria secretion metabolites and the mechanism for the action of bifidobacteria species in CRC prevention could be down-regulation and up-regulation of anti-apoptotic and, pro-apoptotic genes. Conclusions In the present study, different bifidobacteria species showed anticancer activity on colorectal cancer cells through down-regulation and up-regulation of anti-apoptotic and pro-apoptotic genes. However, further studies are required to clarify the exact mechanism of apoptosis induction by bifidobacteria species.

show abstract

The proteins (12 and 15 kDa) isolated from heat‐killed Lactobacillus plantarum L67 induces apoptosis in HT‐29 cells

Cited by 18 publications

References 39 publications

Anticancer Effects of Bifidobacteria on Colon Cancer Cell Lines

Anticancer Effects of Bifidobacteria on Colon Cancer Cell Lines

Anticancer effects of Bifidobacteria on colon cancer cell lines

Anticancer effects of bifidobacteria on colon cancer cell lines

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