The protein corona suppresses the cytotoxic and pro-inflammatory response in lung epithelial cells and macrophages upon exposure to nanosilica

Leibe, Regina; Hsiao, I‐Lun; Fritsch‐Decker, Susanne; Kielmeier, Ulrike; Wågbø, Ane Marit; Voß, Benjamin; Schmidt, Annemarie; Hessman, Sarah Dorothea; Duschl, Albert; Oostingh, Gertie Janneke; Diabaté, Silvia; Weiss, Carsten

doi:10.1007/s00204-019-02422-9

Cited by 55 publications

(51 citation statements)

References 50 publications

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“…In some cases, the suppression of toxicity was observed upon protein corona formation also on amorphous silica nanoparticles (Lesniak et al 2012;Tenzer et al 2013;Leibe et al 2019), but not all studies have confirmed this finding (Hsiao et al 2019;Deville et al 2020). The partial similarities between amorphous and crystalline silica may suggest the occurrence of common surface features on fractured quartz crystals and some amorphous silicas.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of fractured quartz on THP-1 human macrophages: role of the membranolytic activity of quartz and phagolysosome destabilization

et al. 2020

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The pathogenicity of quartz involves lysosomal alteration in alveolar macrophages. This event triggers the inflammatory cascade that may lead to quartz-induced silicosis and eventually lung cancer. Experiments with synthetic quartz crystals recently showed that quartz dust is cytotoxic only when the atomic order of the crystal surfaces is upset by fracturing. Cytotoxicity was not observed when quartz had as-grown, unfractured surfaces. These findings raised questions on the potential impact of quartz surfaces on the phagolysosomal membrane upon internalization of the particles by macrophages. To gain insights on the surface-induced cytotoxicity of quartz, as-grown and fractured quartz particles in respirable size differing only in surface properties related to fracturing were prepared and physico-chemically characterized. Synthetic quartz particles were compared to a well-known toxic commercial quartz dust. Membranolysis was assessed on red blood cells, and quartz uptake, cell viability and effects on lysosomes were assessed on human PMA-differentiated THP-1 macrophages, upon exposing cells to increasing concentrations of quartz particles (10-250 µg/ml). All quartz samples were internalized, but only fractured quartz elicited cytotoxicity and phagolysosomal alterations. These effects were blunted when uptake was suppressed by incubating macrophages with particles at 4 °C. Membranolysis, but not cytotoxicity, was quenched when fractured quartz was incubated with cells in protein-supplemented medium. We propose that, upon internalization, the phagolysosome environment rapidly removes serum proteins from the quartz surface, restoring quartz membranolytic activity in the phagolysosomes. Our findings indicate that the cytotoxic activity of fractured quartz is elicited by promoting phagolysosomal membrane alteration.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity of fractured quartz on THP-1 human macrophages: role of the membranolytic activity of quartz and phagolysosome destabilization

et al. 2020

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“…Suspending particles in the medium may affect the agglomeration state and particle size due to solubility or protein corona. Especially the particle surface interactions with proteins can influence the biological responses as shown by several authors 8,9 . Due to these disadvantages, expert panels recommend the exposure at the ALI [10][11][12] .…”

Section: Methodsmentioning

confidence: 99%

A novel TEM grid sampler for airborne particles to measure the cell culture surface dose

Mülhopt

Schlager²,

Berger³

et al. 2020

Sci Rep

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The applied surface dose is a key parameter for the measurement of toxic effects of airborne particles by air liquid interface exposure of human lung cells. Besides online measurement of the deposited particle mass by quartz crystal microbalance frequently other dose metrics such as particle size distribution, surface and agglomeration state are required. these particle properties and their spatial distribution can be determined by digital processing of micrographs obtained by transmission electron microscopy (TEM). Here, we report the development and characterization of a novel holder for film coated teM copper grids, which allows for sampling under identical geometric and ambient conditions as in a cell culture chamber. the sample holder avoids artefacts by reliable grounding of the grids and improves handling of the grids to prevent damage of the sensitive film. This sample holder is applied during exposure experiments with titanium dioxide nanoparticles. The measured dose of 0.2 µg/cm² corresponds well to the mass loading signal of the quartz crystal microbalance. Additionally, the spatial distribution of particles on the sampling surface shows a good homogeneity of deposition. this novel sampling method allows verifying other dosimetry methods and gives additional information about particle properties and homogeneity of the dose.

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“…Tokgun et al 23 demonstrated that among 6, 15, and 30 nm sizes of aSiNPs, 6 nm-sized nanoparticles had strongest cytotoxic effects on A549 cells, and this cytotoxicity came from dead receptor-mediated induction of apoptosis. Liu et al 24 examined the cell viability of A549 cells after exposing to SiO 2 particles at various concentrations (5,10,20,30,40,60,80, and 100 μg/mL). Results of CCK-8 assay showed that exposures of 10-20 nm aSiNPs resulted in significantly decreased viability of A549 cells, while 5 μm SiO 2 particles did not affect the cell viability obviously.…”

Section: Size-dependent Cytotoxicity Of Asinps Size-dependent Cytotoxmentioning

confidence: 99%

“…9 Meanwhile, aSiNPs could exert cytotoxicity and genotoxicity in many kinds of cell lines, which was considered through the oxidative stress, endoplasmic reticulum stress, autophagy dysfunction or proinflammatory response induced by the particles. 10,11 It is well accepted in nanotoxicology that physicalchemical properties of nanomaterials, such as particle size, surface area, morphology, porosity, aggregation state, crystallinity and other characterizations, should be taken into account when assessing the potential toxicity of nanomaterials in biological systems. As to aSiNPs, a large number of studies have confirmed that these properties could influence the adsorption, distribution, excretion as well as cellular internalization of the particles, which were further associated with the toxic effect in vivo and in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…13,15 In addition, plenty of studies also demonstrated the size dependent toxic effect of aSiNPs-the smaller the particle size was, the greater the toxicity produced. 10,16 However, due to the complexity of experimental conditions and biological systems, there were still some researchers who reported that larger aSiNPs possessed higher toxicity or that no significant difference was observed between aSiNPs with different particle sizes. 17,18 Herein, we provided a systematic literature review to summarize the relationship between the particle size of aSiNPs and its adverse biological effect.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

<p>The Size-dependent Cytotoxicity of Amorphous Silica Nanoparticles: A Systematic Review of in vitro Studies</p>

Dong¹,

Wu²,

Li³

et al. 2020

IJN

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The protein corona suppresses the cytotoxic and pro-inflammatory response in lung epithelial cells and macrophages upon exposure to nanosilica

Cited by 55 publications

References 50 publications

Cytotoxicity of fractured quartz on THP-1 human macrophages: role of the membranolytic activity of quartz and phagolysosome destabilization

Cytotoxicity of fractured quartz on THP-1 human macrophages: role of the membranolytic activity of quartz and phagolysosome destabilization

A novel TEM grid sampler for airborne particles to measure the cell culture surface dose

<p>The Size-dependent Cytotoxicity of Amorphous Silica Nanoparticles: A Systematic Review of in vitro Studies</p>

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