The protein corona from nanomedicine to environmental science

Abstract

The protein corona spontaneously develops and evolves on the surface of nanoscale materials when they are exposed to biological environments, altering their physiochemical properties and affecting their subsequent interactions with biosystems. In this Review, we provide an overview of the current state of protein corona research in nanomedicine. We next discuss remaining challenges in the research methodology and characterization of the protein corona that slow the development of nanoparticle therapeutics and diagnostics, and we address how artificial intelligence can advance protein corona research as a complement to experimental research efforts. We then review emerging opportunities provided by the protein corona to address major issues in healthcare and environmental sciences. This Review details how mechanistic insights into nanoparticle protein corona formation can broadly address unmet clinical and environmental needs, as well as enhance the safety and efficacy of nanobiotechnology strength also influence surface-binding-induced protein unfolding 45 .For example, adsorption-induced protein conformational changes were quantified by targeting and fluorescently labelling exposed amine groups; these residues, which were originally buried inside the tertiary structures of proteins in native conformations, became exposed because of adsorption-induced conformational disorders 48 . Protein conformational changes can impact protein functionality through the exposure of once-concealed functional epitopes or misfolding that results in complete loss-of-protein function, subsequently impacting 1960s

Pivotal role of the protein corona in the cell uptake of fluorinated nanoparticles with increased sensitivity for 19F-MR imaging

“…This approach would allow the formation of a "personalized protein corona", as it has been recently proposed for the use of different nanomedicines with diagnostic and therapeutic purposes. [55][56][57] Experimental Materials PERFECTA was synthesized as previously described. 58 Poly(lactic-co-glycolic acid) (PLGA; Resomer 502H, 50 : 50, 7-17 kDa), polyvinyl alcohol (PVA; 30-70 kDa, 87-90% hydrolyzed), sodium cholate (NaC), and ethyl acetate (EtOAc, purity $ 99.5%) were supplied by Sigma-Aldrich (Germany).…”

An Overview of Nanoparticle Protein Corona Literature

“…[36] Finally, a more robust understanding of the protein corona formed on the surface of lipid-based NPs may facilitate development of efficient, safe, and immune-cell-specific mRNA delivery systems that could pave the way for introduction and widescale use of both current and next-generation robust mRNA-based immunotherapeutics. [59] Markita del Carpio Landry is an associate professor in the Department of Chemical Engineering at the University of California, Berkeley. Her research centers on the development of synthetic nanoparticle-polymer conjugates for neuromodulation in the brain, and for the delivery of functional biomolecules and nutrients into living systems.…”

Nanomedicine Technologies for Diagnosis and Treatment of Breast Cancer

“…Moreover, the clinical translation of nanomedicine in cancer diagnosis and treatment is hindered by the lack of a deep understanding of the biological identity of nanomedicines as well as several regulatory and logistical challenges . The development of nanoparticles involves complex manufacturing processes and requires the development and use of standardized strategies, , along with extensive preclinical and clinical evaluations to ensure safety and efficacy.…”